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BIO311C Exam 2 THE CELL
Mcannelly
172
Biology
Undergraduate 3
10/22/2012

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Term
the three types of cellular life
Definition
archaea, bacteria (both prokaryotic)
and Eukaryota
Term
an aqueous droplet surrounded by a membrane is a ___
Definition
protocells
Term
lipids added to water can spontaneously form ___
Definition
vesicles
Term
protists are ___ celled and ____karyotes
Definition
single celled eukaryotes
Term
1. Cell (plasma) membrane
2. Semi-fluid interior = cytoplasm (aka cytosol)
3. DNA organized into chromosome/s
4. Ribosomes

what are these 4 attributes?
Definition
These 4 elements are common to all cells
Term
the difference in prokaryotes and eukaryotes is
Definition
eukaryotes have a true nucleus & membrane bound organelles
Term
nuceloids are found in ___ cells
Definition
prokaryotic cells ( DNA in unbound region)
Term
the differences between Archaea and Bacteria are
Definition
membrane lipids difffer, archaea has extremophiles, cell walls are made differently
Term
Exchange of oxygen, nutrients, and wastes occurs at the
Definition
plasma membrane
Term
The ____ the surface to volume ratio, the less effective is exchange of gases & nutrients per unit of volume
Definition
lower
Term
To get big, increase _____ not cell size
Definition
number of cells
Term
light microscopy is special because you can see
Definition
cells while theyre still alive
Term
__ ____ Separates cellular components based on size and density
Definition
cell fractionation
Term
plasmodesmata only occurs in ___ cells
Definition
plant
Term
lysosomes, centrosomes & centrioles, flagella
These are only found in
Definition
animal cells
Term
The _____ is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell
Definition
plasma membrane
Term
the nucleus can be seen in ____ microscopy
Definition
light
Term
Ribosomes use the information from the DNA to make ____
Definition
proteins
Term
__ ___ encloses the nucleus, separating it from the cytoplasm
Definition
nuclear envelope
Term
____ regulate the entry and exit of molecules from the nucleus
Definition
pores
Term
The shape of the nucleus is maintained by the___ ____, which is composed of protein filaments
Definition
nuclear lamina
Term
The ____ is located within the nucleus and is the site of ribosomal RNA (rRNA) synthesis
Definition
nucleolus
Term
____ condenses to form discrete chromosomes
Definition
chromatin
Term
Ribosomes carry out protein synthesis in two locations:
Definition
1. in the cytosol
2. on the outside of the ER or nuclear envelope
Term
Nuclear envelope
Endoplasmic reticulum (ER)
Golgi apparatus
Lysosomes
Vacuoles
Plasma membrane

These are all components of the
Definition
endomembrane system
Term
phospholipids are made in ____ ER
Definition
smooth
Term
Smooth vs Rough ER
Definition
smooth doesn't have ribosomes
Term
Synthesizes lipids
Metabolizes carbohydrates
Chemically alters exogenous compouds
Drug metabolism
Detoxifies poison
Stores calcium
These are all the functions of the ___ ___ ____
Definition
the 4 functions of Smooth ER
Term
Polypeptide often combined with carbohydrate is a ____
Definition
glycoprotein
Term
the flattened membrane sacs of the Golgi are
Definition
cisternae
Term
Further modification of the glycoproteins received from the ER
Modification of phospholipids
Manufactures certain macromolecules
Sorts and packages materials into transport vesicles

These are the functions of the
Definition
Golgi Apparatus
Term
cis face is the ____ side of the golgi
Definition
receiving
Term
Last step before protein leaves golgi = ___
Definition
tagging
Term
Enzymes that break down macromolecules are called
Definition
hydrolytic enzymes
Term
a membranous sac of hydrolytic enzymes that can digest macromolecules with an acidic interior
Definition
lysosomes
Term
engulfing smaller organisms or food particles is a process known as ____
Definition
phagocytosis
Term
Lysosomes have enzymes which can break down the cell’s own organelles and macromolecules, a process called
Definition
autophagy
Term
central vacuoles hold
Definition
organic compounds and water
Term
___ and ____:
Are not part of the endomembrane system
Have a double membrane
Have proteins made by free ribosomes
Contain their own circular DNA
Definition
Ribosomes and Mitochondria
Term
Mitochondria is necessary for the ___ reactions
Definition
aerobic
Term
"powerhouse of the cell" is
Definition
the mitochondria
Term
The chloroplast is a member of a family of organelles called
Definition
plastids
Term
___ are Specialized metabolic compartments bounded by a single membrane
Functions:
Detoxify harmful compounds (alcohol) by converting to hydrogen peroxide (H2O2)
Then convert the hydrogen peroxide to water
Definition
peroxisomes
Term
Network of fibers extending throughout the cytoplasm
Organizes the cell’s structures and activities
Anchors many organelles
Definition
cytoskeleton
Term
the functions of the cytoskeleton are
Definition
support and movement within the cell
Term
Hollow rod
Tubulin
Thickest
these are charcteristics of ___ fibers
Definition
microtubules
Term
actin is part of the ___ type of fibers
Definition
microfilament
Term
Shaping the cell – resist compression
Guiding movement of organelles
Separating chromosomes during cell division
Major components of Centrioles & Centrosomes Flagella
Cilia
these are all functions of
Definition
microtubules
Term
____ is a microtubule-organizing center.
Definition
centrosome
Term
A motor protein called ____ drives the bending movements of a cilium or flagellum
Definition
dyenin
Term
microfilaments have ___ & ___ roles
Definition
structural and movement
Term
microfilaments are ___ bearing
Definition
tension
Term
Microfilaments that function in cellular motility contain the protein _____ in addition to actin
Definition
myosin
Term
______ are more permanent parts of the cytoskeleton than the other two classes
They support cell shape and fix organelles in place
Definition
intermediate microfilaments
Term
Functions in signal reception
Important in kidney, brain function and embryonic development.
Defect in it = polycystic kidney disease
Definition
primary cilium
Term
_____ make up cell walls in Bacteria
Definition
peptidoglycan
Term
Gram ___ has simpler cell wall, lots of peptidoglycan
Definition
positive
Term
thin layer between primary walls of adjacent cells (pectin)
Definition
middle lamella
Term
allow passage of water and small solutes (and sometimes proteins and RNA) from cell to cell
only in plants
Definition
Plasmodesmata
Term
a type of glycoprotein. Has small protein core with lots of polysaccharide (up to 95%)
Definition
proteoglycan
Term
collagen, fibronectin and proteoglycan make up the
Definition
Extra Cellular Matrix
Term
ECM proteins bind to receptor proteins in the plasma membrane called
Definition
integrins
Term
membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid are ___ junctions
Definition
tight
Term
(communicating junctions) provide cytoplasmic channels between adjacent cells are ___ junctions
Definition
gap
Term
(anchoring junctions) fasten cells together into strong sheets are called
Definition
desmosomes
Term
____ junctions prevent fluid from moving across a layer of cells
Definition
tight
Term
is the boundary that separates the living cell from its surroundings
Definition
plasma membrane
Term
Simple
Osmosis
Facilitated
are all mechanisms of ___ transport
Definition
passive
Term
_____ in the plasma membrane can move within the bilayer.
Definition
phospholipids
Term
___ ___ is a specialized preparation technique that splits a membrane along the middle of the phospholipid bilayer
Definition
freeze fracture
Term
____ ___ depends on what types of fatty acids compose the hydrophobic tails of the phospholipids
Definition
membrane fluidity
Term
membrane fluidity is ___ dependenet
Definition
temperature
Term
the role of cholesterol is a
Definition
temperature buffer
Term
____ stabilizes membranes by decreasing fluidity at
body temperature and by interfering with tight packing of
phospholipids at cooler temperatures
Definition
cholesterol
Term
dynein is a motor protein associated with
Definition
microtubules
Term
the membrane proteins of the fluid mosaic model are amphipathic which means
Definition
they have both hydrophillic and hydrophobic regions
Term
membrane fluidity depends on
Definition
what types of fatty acids compose the hydrophobic tails of the phospholipids
Term
___ determine most of membrane's specific functions
Definition
proteins
Term
____ proteins are bound to the surface of the membrane
Definition
peripheral
Term
____ proteins penetrate the hydrophobic core.
Definition
integral
Term
Integral proteins that span the membrane are called _____ proteins
Definition
transmembrane
Term
1. transport
2. enzyme activity
3. signal transduction
4. cell to cell recognition
5. interception
6. attachment to cytoskeleton and ECM
these are 6 major functions of _____ _____
Definition
membrane proteins
Term
This asymmetrical distribution of proteins, lipids, and associated carbohydrates in the plasma membrane is determined when the membrane is built by the ___ and the ___
Definition
ER and the Golgi
Term
Nutrients, water and oxygen go into the membrane via selective permeability and ___ & ___ come out
Definition
waste & CO2
Term
concentrations of _____ ions are regulated by selective permeability, sometimes against their concentration gradient
Definition
inorganic ions
Term
polar, charged molecules like sugars and ions cross the membrane ____
Definition
very slowly. hard.
Term
does water need help to cross the membrane>?
Definition
yes.
Term
channel proteins form a
Definition
water filled pore (uses channel called aquaporins for water)
Term
carrier proteins are able to ___ ___ which allows for no direct connection with outside and in
Definition
change shape
Term
in passive transport there is _____ and you go from ___ to ___ concentration. Two examples are:
Definition
no energy needed. you go from HIGH TO LOW concentration and two examples are diffusion and osmosis
Term
Active Transport: ____ needed and you go from ___ to ___. Two examples are
Definition
Energy needed. You can go from low to high or high to low. Examples are: Na+ K pump and H+ pump
Term
Molecule size
Temperature
Medium
Volatility
these are the four factors that affect:
Definition
diffusion
Term
Tendency of molecules to spread within the available space
Definition
simple diffusion
Term
water diffuses across membrane from ___ concentration to ____ concentration
Definition
low to high concentration
Term
___ is the ability of a solution to cause a cell to gain or lose water
Definition
tonicity
Term
____ solution: Solute concentration is greater than that inside the cell; cell loses water
Definition
hypertonic
Term
____ solution: Solute concentration is less than that inside the cell; cell gains water
Definition
hypotonic
Term
Diffusion requires a ____ ____ if the membrane is not permeable to the solute
Definition
membrane protein
Term
___ & _____ proteins carry out facilitated diffusion
Definition
carrier and channel proteins
Term
Facilitated diffusion is still passive because the solute
Definition
moves down its concentration gradient
Term
active transport is performed
Definition
specific proteins embedded in the membranes
Term
3 types of active transport are: __porter, ___porter, __porter
Definition
uniporter-1 solute 1 direction
symporter- 2 solutes one direction
antiporter- 2 solutes different directions
Term
Concentration gradient established by Na+/K+ pump favors movement of ___ into cell
Definition
Na+
Term
primary active transport uses ____ directly.
Secondary active uses ____
Definition
ATP; energy supplied by electrical gradients and ion channels
Term
The ____ pump is the main electrogenic pump in plants, fungi, & bacteria
Definition
proton
Term
Plants use the gradient of ____ __generated by proton pumps to drive active transport of nutrients into the cell
Definition
hydrogen ions
Term
___ ___ is the voltage difference across a membrane
Definition
membrane potential
Term
Electrochemical gradient can power
Definition
secondary active transport and signals in neurons
Term
can be physical stimulus such as light or a chemical such as an odor molecule
Definition
signal
Term
is the sequence of events that leads to the cell’s response to the signal
Definition
signal transduction pathway
Term
local signaling is
Definition
direct cell to cell contact, adjacent cells
Term
___ signaling produces hormones that are carried by the circulatory system to remote locations within the organism
Definition
endocrine
Term
besides endocrine, another type of long distance signaling is ____. which is electrical transmission along neurons
Definition
neural
Term
the binding of ligand and receptor is specific and exclusive. the only exceptions are
Definition
drugs and inhibitors
Term
membrane bound receptors vs cytoplasmic receptors
Definition
membrane bound are big and polar
cytoplasmic are small and nonpolar
Term
If ligand is lipophobic, it binds with
Definition
a cell membrane receptor
Term
enzyme that catalyzes phosphorylation and transfers the phosphate groups
Definition
kinase
Term
indirect cell signaling involves
Definition
the second messenger
Term
protein phosphotases act as a
Definition
switch on/off
Term
direct receptor/ligand binding occurs with __ channels
Definition
ion channels
Term
Signal transduction pathways typically involve
Definition
activation of proteins within the cell by phosphorylation
Term
3 main second messengers are
Definition
Ca2+, IP3 and cAMP
Term
IP3 acts on ER to
Definition
release Ca2+ which then also acts as a second messenger
Term
____ is the enzyme that dephosphorylates
Definition
AKT
Term
the capacity to cause change or do work is
Definition
energy
Term
all cells use energy. whether its to _____ __ or to ___ ______
Definition
break bonds, transform energy
Term
energy transformations involve ____ pathways
Definition
metabolic
Term
____ pathways consume energy to build complex molecules from simpler ones
Definition
anabolic
Term
pathways release energy by breaking down complex molecules into simpler compounds
Definition
catabolic

example is cellular respiration
Term
When energy is converted from one form to another, some of that energy becomes unavailable to do work. this is the
Definition
2nd law of thermodynamics
Term
every energy transfer increases the ____
Definition
entropy of the universe
Term
entropy means that ___ increases
Definition
disorder
Term
Energy flows into an ecosystem in the form of ___ and exits in the form of ____
Definition
light; heat
Term
earth is an ___ system
Definition
open
Term
energy able to do work is called
Definition
free energy delta G!
Term
total energy is called
Definition
enthalpy
free energy + entropy
Term
delta g =
Definition
delta h (entropy) - T(temp)*delta S(entropy
Term
if delta g is negative then free energy is ____
Definition
released
Term
delta s is the change in entropy. Large increases in entropy make ΔG more ___
Definition
negative
Term
at chemical equilibrium delta g=
Definition
0
Term
During a spontaneous change, free energy ____ and the stability of a system _____
Definition
free energy decreases and system stability increases.
Term
a catabolic pathway in a cell releases ____ _____ in a series of reactions
Definition
free energy
Term
Cyanide binds to at least one molecule involved in producing ATP. If a cell is exposed to cyanide, most of the cyanide will be found within the
Definition
mitochondria
Term
In what way do the membranes of a eukaryotic cell vary?
Definition
Certain proteins are unique to each membrane.
Term
If an enzyme in solution is saturated with substrate, the most effective way to obtain a faster yield of products is to add
Definition
add more of the enzyme
Term
If an enzyme is added to a solution where its substrate and product are in equilibrium, what will occur?
Definition
nothing. the reaction will stay at equilibrium
Term
Binding of a signaling molecule to which type of receptor leads directly to a change in the distribution of ions on opposite sides of the membrane?
Definition
ligand gated channels
Term
Lipid-soluble signaling molecules, such as testosterone, cross the membranes of all cells but affect only target cells because
Definition
intracellular receptors are only in target cells
Term
Which observation suggested to Sutherland the involvement of a second messenger in epinephrine's effect on liver cells?
Definition
glycogen breakdown was observed only when epinephrine was administered to intact cells
Term
Consider two cells with the same volume but with very different surface areas due to differences in their shapes. The cell with the larger surface area is likely to _____.
Definition
be involved in the rapid uptake of compounds from the cell's environment
Term
Prokaryotic cells are distinguished by
Definition
their lack of organelles
Term
_____ are sites where protein synthesis occurs.
Definition
ribosomes
Term
A substance moving from outside the cell into the cytoplasm must pass through _____
Definition
the plasma membrane
Term
In addition to the fundamental structures required to be defined as a cell, a particular cell also has a nucleus and chloroplasts. Based on this information, this cell could be _____.
Definition
a protistal or a plant cell
Term
Chromosomes only appear as a cell is about to divide.
True or False?
Definition
False
Term
What group is primarily involved in synthesizing molecules needed by the cell?
Definition
smooth and rough ER, ribosomes
Term
A dish of animal cells was grown in the presence of radioactive phosphorous. The phosphorous largely ended up in nucleotides inside the actively growing animal cells. In which cellular structure(s) would you predict the majority of the radioactive phosphorous to accumulate?
Definition
the nucleus b/c it needs a lot of nucelotides to perform its functions
Term
You would expect a cell with an extensive Golgi apparatus to _____.
Definition
secrete a lot of protein
Term
what is primarily involved in cell motility?
Definition
the cytoskeleton
Term
Consider a protein that is made in the rough endoplasmic reticulum. You observe that when the synthesis of the protein is completed, the protein is located in the ER membrane. Where else in the cell might this protein be found?
Definition
embedded in the plasma membrane functioning in the transport of molecules into the cell
Term
Proteins that are inserted into the rough ER membrane are _____ proteins, not soluble proteins to be excreted.
Definition
membrane
Term
A protein that ultimately functions in the plasma membrane of a cell is most likely to have been synthesized _____
Definition
in the rough ER
Term
Chloroplasts and mitochondria are thought to be of prokaryotic origin. One piece of evidence that supports this hypothesis is that these organelles contain prokaryotic-like ribosomes. These ribosomes are probably most similar to ribosomes found _____.
Definition
in bacterial cells
Term
whats a reason for grouping the peroxisomes with chloroplasts and mitochondria?
Definition
none of these organelles are part of the endomembrane system
Term
The observation that chloroplasts and mitochondria each contain their own DNA and synthesize some of the proteins that function in these organelles suggests that chloroplasts and mitochondria _____
Definition
must divide whenever the cell divides
Term
Which of the following organelles might be found inside other organelles?
Definition
ribosomes
Term
transport vesicles are NOT
Definition
organelles
Term
does RNA have anything to do with cytoskeleton?
Definition
NO
Term
Components of the cytoskeleton often mediate the movement of organelles within the cytoplasm.
True or False?
Definition
TRUE
Term
Both the ECM and the plant cell wall are composed of varying mixtures of proteins and carbohydrates.
True or False?
Definition
true
Term
during ____ sister chromatids separate
Definition
anaphase 2
Term
during ______ a spindle forms
Definition
prophase 2
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