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Bio: 150 Chapter 8
Photosynthesis
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Biology
Undergraduate 1
04/28/2014

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Term
Photosynthesis,  the  process  by  which  organisms  ______  ______  from  sunlight  and  use  it  to build  
______  ______  that  are  rich  in  ______  ______.
Definition
Term
The  energy  used  by  most  living  cells  comes
ultimately  from  the  Sun  and  is  captured  by  ______,  _____,  and  _____  through  the  process  of  photosynthesis.

 

Definition
Plants, Algae and Bacteria
Term
What  are  the  beginning  reactants  and  ending  products  of  photosynthesis?
Definition

6 CO2 + 12H2O + Light ---> C6H12O6 + 6H2O + 6O2

carbon diox + Water + Light ---> Glucose + Water +Oxy

Term
What  is  anoxygenic  photosynthesis?    What  organisms  use  this  process?    What  is  oxygenic  photosynthesis?    What  organisms  use  this  process?

 

Definition

Anoxygenic Photosynthesis does not produce oxygen and found in four different bacteria groups: purple bacteria, green sulfur bacteria, green non-sulfur bacteria, and heliobacteria

 

Oxygenic does produce oxygen and is found in cyanobacterian, seven groups of algae and basically all land plants

Term

What  structures  in  plants  carry  out  photosynthesis?

Definition
In plants it takes place in the leaves in chloroplasts.
Term
What  are  the  three  stages  of  photosynthesis? Which of these  are  light-­‐dependent  and  which  are  
light-­independent?
Definition
  1. Capturing energy from sunlight
  2. Using energy to make ATP and to reduce the compound NADP+, and electron carrier to NADPH; and
  3. Using the ATP and NADPH to power the synthesis of organic molecules from CO2 in the air

 

The first two are light-dependent and the third in ligh-independent

Term
Draw  and  label  a  picture  of  a  chloroplast.    Where  does  each  step  of  photosynthesis  take place in this organell?
Definition
Pg. 148 figures 8.1
Term
What must be present for carbon fixation reactions to occur?
Definition
As long as ATP and NADPH are available the carbon fixation reactions can occur either in the presence or absence of light
Term

How are the reactions for respiration and photosynthesis related?

Definition

The equation for photosynthesis is reverse for the respiration reaction equation.

 

Term
What  similarities  are  there  in  mitochondria  and  chloroplast  structure?
Definition
both the complex structure internaly and externaly contribute to it's functions.
Term
In  respiration  electrons  are  passed  from  one  molecule  to  another;  is  this  the  case  in  photosynthesis?  Why  or  Why not?
Definition
THe excited electron is not transfered physically---rather, its energy passes from one molecule to another. This passage is similar to the transfer of kinetic energy.
Term
Does  O2 come  from  the  splitting  of  CO2 or  H2O  during  photosynthesis?
Definition
Electrons lost during phostosynthesis are replaced by the oxidation of water, producing O2 as a by product.
Term
What  structure  must  be  present  to  absorb  light  energy  to  make  use  of  the  energy?
Definition
For plants to make energy from sunlight, some biochemical structure must be present in chloroplasts and thylakoids that can absorbe this energy. Molecules that absorbe light energy in the visible range are termed pigments.
Term

In pigments, the color we see is the color  that  is  _____, that  is,  it  is  _____.

Definition
Not Absorbed; Reflected
Term
What  does  light  wavelength  impact?    What  is  an  electromagnetic spectrum?
Definition
The wave nature of light produces an electromagnetic spectrum that differentiates light based on its wavelenth
Term
How  is  the  energy  of  a  photon  related  to  the  wavelength  of  light?
Definition
A partical of light termed a photon, acts like a discrete bundle of energy. We use the wave concept of light to understand different colors of light and the partical nature of light to understand the energy transfers that occur during photosynthesis.
Term
What  is  the  photoelectric  effect?
Definition
A beam of light is able to remove electrons from certain molecules, creating an electrical current. This phemomenon is called the photoelectic effect.
Term
How  does  wavelength  of  light  impact  the  strength  of  the  photoelectric  effect?
Definition
The stenbth of the photoelectric effect depends on the wavelenth of light; that is, short wave lengths are far more effective than long ones in producing the photoelectric effect because they have more energy.
Term
What  is  the  role  of  chloroplasts  regarding  the  photoelectric  effect?
Definition
In photosynthesis, chloroplasts are acting as photoelectric devices: they absorbe sunlight and transfer the excited electrons to a carrier.
Term
What  is  a  molecule’s  absorption  spectrum?
Definition
A specific atom can absorb only certain photons of light---namely, those that correspond to the atom's available energy levels. As a result, each molecule has a characteristic absorption spectrum, the range and efficiency of photons it is capable of absorbing.
Term
What types of pigments are used in green plant photosynthesis?
Definition
Chlorophyll a and chlorophyll b preferentially absorbe violet-blue and red light.
Term
What  are  the  two chlorophylls  in  plants?    What  light  do  they  absorb?  Reflect? How  are  they  different  from  each Other?
Definition
Chlorophyll a and b. Chlorophyll a is the main photosynthetic pigment in plants and cyanobacteria and the only pigment that can act directly to convert light energy to chemical energy. Chlorophyll b, acting as an accessory pigment, or secondary light-absorbing pigment, complements and adds to the light absorption of chlorophyll a.
Term

Is light absorbed by independent pigment molecules or clusters of chlorophyll molecules? What data supports this Finding?

Definition
Light is absorbed, not by independent pirment moluecules, but rather by clusters of chlorophyll and accessory piment molecules. expiriement proving this pg. 154
Term
What is a photosystem?
Definition
In all cholorplasts and in all but one photosynthetic prokaryotes, light is captured by a photosystem. Each photosystem is a network of chlorophyll a molecules, accessory pigments, and associated proteins held together within a protein matrix on the surface of a photosynthetic membrane.
Term
What is a reaction center chlorophyll?
Definition
is a transmembrane protein pigment complex.
Term
What  is  an  antenna  complex?
Definition
also called light-harvesting complex, which accurately describes its role.
Term
How  are  these  threecomponents  related? What  gets  passed  between  each?    How  does  this  transfer energy?
Definition
the light-harvesting complex captures photons from sunlight and channels them to the reaction center chlorophylls. More explanation pg. 155
Term
Where  do  the  light-dependent  reactions  of  photosynthesis  occur?    How  does  this  differ  in  prokaryotes and eukaryotes ?
Definition
these reactions take place on the membrane. In photosynthetic bacteria, the plasma membrane itself is the photosynthetic membrane. In many bacteria, the plasma membrane folds in on itself repeatedly to produce and increased surface area. In plants and algea, photosynthesis is carried out by chloroplasts which are thought to be evolutionary desendents of photosynthetic bacteria.
Term
What are the four stages of Thylakoid reaction?
Definition
  1. Primary photoevent. A photon of light it captured by a pigment. This primary photoevent excites an electron within the pigment.
  2. Charge seperation. This excitation energy is transfered to the reaction center, which transfers an energetic electron to an acceptor molecule, initiating electron transport.
  3. Electron Transport. The excited electrons are shuttled along a series of electron carrier molecules embedded within the photosynthetic membrane.
    Several of them react by transfering protons across the membrane, generating a proton gradient. Eventually the electrons are used to reduce a final acceptor, NADPH.
  4. Chemiosmosis. The protons that accumulate on one side on the membrane now flow back across the membrance through ATP synthase where chemiosmotic synthesis of ATP takes place, just as it does in aerobic respiration.
Term
Are  these  part  of  the  light-­‐dependent  or  light-­‐independent  reactions?    Why?
Definition
These four stages make up the light-dependent reactions mentioned earlier. Steps 1-3 represent the stage of capturing energy from light; step 4 is the stage of producing ATP.
Term
What is cyclic photophosphorylation? Why is it named as such? How many photosystemsystems are involved?
Definition
In bacteria a single photosystem is used that generates ATP via electron transport. This process then returns the electrons to the reaction center. For this reason it is called the cyclic photophosphorylsation.
Term
How  many  photosystems  are  linked  in  chloroplasts?    What  are  they  called?    How  do  they  differ?    What  electron  carriers  connect  these  photosystems?
Definition

Chloroplasts have two connects photosystems. Photosystem I and II.This overcomes the limitations of cyclic photophosphorylation by providing an alternative source of electrons from the oxidation of water.

 

PI has an absorbtion peak of 700 nm, so its reaction center pigment is called P700. PII has an absorbtion peak of 680nm, so its reaction center pigment is P680. This photosystem can generate an oxidation potential high enough to oxidize water.

Term
What is a Z diagram?
Definition
this diagram illustrates the two electron-energizing steps, one catalyzed by each photosystem. more on pg 157
Term
How do light reaction generate NADPH and ATP?
Definition
Photosystem II acts first. High-energy electrons generated by photosystem !! are used to synthesize ATP and are then passed to photosystem I to drive the production of NADPH. For every pair of electrons obtained from a molecule of water, one molecule of NADPH and slightly more than one molecule of ATP are produced.
Term
What is a carbohydrate? How does the cell build carbohydrates?
Definition

Carbohydrates contain many C--H bonds are are highly reduced compared with CO2. To build carbohydrates cells use energy and a source of electrons produced by the light dependent reactions of thylakoids: Energy and Reaction Potential.


Energy:
ATP (provided by cyclic and non cyclic photophosphorylation) drives the endergonic reactions.

Reaction Potential. NADPH (provided by photosystem I) provides a source of protons and the energetic electrons needed to bind them to carbon atoms. Much of the light energy captured in photosynthesis ends up invested in the energy-rich C--H bonds

Term
What is carbon Fixation?
Definition
Reactions that form a cycle of enzyme-catalyzed steps much like the krebs cycle of respiration. Unlike the Krebs cycle however, carbon fixation is geared toward producing new compounds so the nature of the cycles is quite different.
Term
What is the Calvin Cycle? Where does it occur? What are the energy sources?
Definition
The cycle of reactions that allow the carbon fixation is called the Calvin Cycle, also called C3 photosynthesis because of the three carbon atoms contained in the first intermediate of the cycle. pg. 161 for more info
Term
Without  going  into  details  on  enzyme  names,  what are  the  three  phas
es  of  the  Calvin  cycle  and  what  is  the  purpose  and  

product  of  each?

Definition
  1. Carbon Fixation
  2. Reduction
  3. Regeneration

The carbon fixation reaction generates two molecules of the 3-carbon acid PGA; PGA is then reduced to G3P by reactions that are essentially a reverse of part of glycolysis; finally the PGA is used to regenerate RuBP. Three turns around the cycle incorperat enough carbon to produce a new molecule of G3P, and six turn incorporate enough carbon to synthesize one glucose molecule.

Term
What does the enzyme rubisca do?
Definition
Ribulous Bisphosphate carbon/oxygenase (abbreviated to Rubisca) carries out the carbon fixation reaction. Pg. 161 for more info.
Term
What are the net imputs and outputs of the Calvin cycle?
Definition
???
Term
Is glucose a direct output? Why or Why not?
Definition
???
Term
How does the Calvin Cycle compare with glycolysis?
Definition

Reactions are reversed similar to glycolysis. pg. 162

 

Term
What is the energy cycle relatioshit between photosynthesis and respiration?
Definition
The energy-capturing metabolism of the chloroplasts studied in this chapter and the mitochondria studied in chapter 7 are intimatley related. Photosynthesis uses the products of respiration as starting substrates, and respiration uses the products of photosynthysis as starting substrates. The production of glucosen from G3P even uses part of the ancient glycolic pathway, run in reverse. Also, the principlal proteins involved in electron transport of ATP productions in plants are evolutionarily related to those in mitochondria.
Term
1.) The light dependent reactions of photosynthesis are responsible for the production of...
Definition
ATP and NADPH
Term
2.) What region of a chloroplast is associated with the capture of light energy?
Definition
Thylakoid Membrane
Term
3.) The colors of light that are most effective for photosynthesis are...
Definition
Red, Blue and Violet
Term
4.) During noncyclic photosynthesis, photosystem I functions to ___, and photosynthesis II functions to ___.
Definition
Reduce NADP+; Oxidize H2O
Term
5.) How is a reaction center pigment in a photosystem different from a pigment in the atena complex?
Definition
The reaction center pigment loses and electron when it absorbes light energy
Term
6.) the ATP and NADPH from the light reactions are used...
Definition
During the reactions of the Calvin Cycle to produce glucose
Term
7.) The carbon fixation reaction converts...
Definition
Inorganic carbon into an organic acid
Term
1.) The overall flow of electrons in light reactions are from...
Definition
H2O to CO2
Term
2.) Where in the chloroplast would you find the highest concentration of protons?
Definition
In the lumen of the Tykaloid
Term
3.) How does the reaction center of photosystem I regain an electron during non cyclic photosynthesis?
Definition
The electron is donated form photosystem II
Term
4.) If the Calvin Cycle runs through six turns...
Definition
Enough carbon will be fixed to make one glucose, but they will not all be in the same molecule
Term
5.) What are similarities between structure and function of mitochondria and chloroplasts?
Definition

They both create internal proton gradients by electron transport and they both have an outer membrane and an inner membrane system.

 

Term
Carbon Fixation by the C4 pathway produces...
Definition
an organic acid, but a 4-carbon one not a 3-carbon.
Term
7.) If the thylakoid membrane became leaky to ions, what would you predict to be the result on the light reactions?
Definition
It would stop ATP production
Term
8.) The overall process of photosynthesis...
Definition
results in the reduction of CO2 and the oxidation of H2O
Term
Diagram the relationship between the reactants and products of photosynthesis and respiration
Definition
Figure 8.19 diagrams this relationship. The oxygen produced by photosynthesis is used is used as a final electron acceptor for electron transport in respiration. The CO2 that results from the oxidation of glucose (or fatty acids) is incorperated into organic compunds via the Calvin Cycle. Respiration also produces water, while photo synthesis consumes water.
Term
Do plant cells need mitochondria? Explain.
Definition
Yes, plants use their chloroplasts to convert light energy into chemical energy. During light reactions ATP and NADPH are created, but these molecules are consumed during the calvin cycle and are not available for the cell's general use. The G3P produced by the Calvin cycle stores the chemical energy from the light reactions with in its chemical bonds. Ultimately, this energy is stored in glucose and retrieved by the cell through the process of glycolisis and cellular respiration.
Term
How does the Calvin Cycle compare with glycolisis?
Definition
A portin of the Calvin cycle is the reverse of glycolisis (the reduction of 3-phosphoglycerate to glyceraldehyde-3-phosphate).
Term
8.5) If the thylakoid were leaky to protons would ATP still be produced? Would NADPH?
Definition
Without a proton gradient, synthesis of ATP by chemiosmosis would be impossible. However, NADPH could still be synthesized because electron transport would still occur as long as photons were still being absorbed to begin the process
Term
8.4) Why were photosystems an unexpected finding?
Definition
Before the discovery of photosynthesis, we assumed that each chlorophyll molecule absorbed photons resulting in excited electrons.
Term
8.3) What is the difference between an action spectrum and an absorption spectrum?
Definition
The action spectrum of photosynthesis refers to the most effect wavelengths. The absorption spectrum for an individual pigment shows how much light is absorbed at different wave lenghts.
Term
8.1) How is the structure of the chloroplast similar to the mitochondria?
Definition
Both chloroplats and mitochondria have an outer membrane and an inner membrane. The inner membrane in both forms an elaborate structure. These inner membrane systems have electron transport chains that move protons across the membrane to allow for the synthesis of ATP by chesiomosis. They also both have a soluable compartment in which a variety of enzymes carry out reactions.
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