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Bio Lab Exam 1
Bio Lab Exam 1
72
Biology
Undergraduate 1
10/18/2011

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Term
Volume
Definition
length x width x height
Term
Area
Definition
Length x width
Term
Variability
Definition
A measure of how likely data will vary
Term
Standard Deviation
Definition
A measure of variability that shoes how much variation there is from the average
Term
Solution
Definition
A homogeneous mixture
Term
PH
Definition
measure of the acidity or basicity of a water solution
Term
Buffers
Definition
Mixtures of weak acid and a weak base that can be combine with a strong acid or base to limit changes in PH
Term
Dehydration synthesis
Definition
Removing water
Term
Hydrolysis
Definition
water is recombined
Term
Positive Control
Definition
This control group will have the reaction you expect.
Term
Negative control
Definition
The control group: Negative control should not have the reaction you expect of the positive control
Term
Reducing Sugars
Definition
Sugars that contain aldehyde groups that are oxidized to carboxylic acids
Term
Benedicts Regent
Definition
Tests for reducing sugars
Term
Biuret Test
Definition
Tests for peptide bonds
Term
Peptide bond
Definition
is a covalent chemical bond formed between two molecules when the carboxyl group of one molecule reacts with the amino group of the other molecule
Term
Iodine
Definition
tests for starch
Term
Dische diphenylamine
Definition
Test for DNA
Term
Spectrophotometry
Definition
Tests for wavelengths of light
Term
Blank
Definition
A water solution to bring the spectrophotometer to 0
Term
Sample
Definition
The solution checked in spectrophotometer
Term
Filter
Definition
Adjusts wavelengths
Term
Absorbance
Definition
The amount of radiation retained from the sample
Term
Transmittance
Definition
The amount of radiation that passes through the sample.
Term
Absorption spectrum
Definition
The wavelength of the sample
Term
Standard Curve
Definition
A graph showing a chemicals concentration vs it's absorbance of a wavelength of light.
Term
Enzyme
Definition
Biological Catalysts, most are proteins with individual shapes.
Term
Active Site
Definition
part of an enzyme where substrates bind and undergo a chemical reaction
Term
Substrate
Definition
a substrate is the molecule upon which an enzyme binds
Term
Cofactors/Coenzymes
Definition
Vitamins and small molecules that aid in the binding
Term
Enzyme-substrate complex
Definition
formed when enzymes and substrates bond.
Term
peroxidase
Definition
Large protein (enzyme) containing amino acids
Term
Derivative
Definition
A slope in your raw data graph
Term
Denaturation
Definition
A permanent structural change
Term
Microscope Contrast
Definition
Difference between the lightest and darkest parts of the image
Term
Microscope Resolution
Definition
The ability to distinguish two points as different points
Term
Condenser Lens
Definition
located below the specimen, focuses the light from the light source onto the specimen
Term
Condenser iris diaphragm
Definition
The knob that allows you to adjust to regulate the light on the specimen
Term
Ocular
Definition
The lens you look through
Term
Objectives
Definition
The 3 rotating lenses
Term
Ocular micrometer
Definition
The line in the lens
Term
Cytoplasm
Definition
is a gel-like substance residing between the cell membrane holding all the cell's internal sub-structures
Term
Plasma Membrane
Definition
a biological membrane that separates the interior of all cells from the outside environment
Term
Cell Wall
Definition
The cell wall is the tough, usually flexible but sometimes fairly rigid layer that surrounds some types of cells
Term
Capsule
Definition
large structure of some prokaryotic cells, such as bacterial cells. It is a layer that lies outside the cell wall of bacteria.
Term
Flagella
Definition
tail-like projection that protrudes from the cell body of certain prokaryotic and eukaryotic cells
Term
Prokaryotes
Definition
Have no nucleus or any other membrane bound organelles
Term
pili
Definition
hair like outgrowths in prokaryotes
Term
ribosomes
Definition
small particles involved in protein synthesis
Term
cyanobacteria
Definition
The largest prokaryotes
Term
Vacuole
Definition
Vacuoles are essentially enclosed compartments
Term
Middle Lamella
Definition
layer which cements the cell walls of two adjoining cells together.
Term
cristae
Definition
The inner membrane when folded to hold respiratory enzymes
Term
Plastids
Definition
Organelles where food is made and stored.
Term
Brownian movement
Definition
The random motion in which molecules passively move around.
Term
Polar and nonpolar molecules
Definition
Polar have areas both positively and negatively charged. Non polar have neither.
Term
Dialysis
Definition
The separation of dissolved substances
Term
hypotonic
Definition
More solute than solvent
Term
hypertonic
Definition
Less solute than solvent
Term
isotonic
Definition
Equal solute and solvent
Term
Water potential
Definition
The potential energy of water per unit volume
Term
plasmolysis
Definition
The shrinking of the cytoplasm of a cell in response to diffusion of water out of the cell
Term
Cellular Respiration
Definition
Cellular respiration is the set of the metabolic reactions and processes that take place in the cells of organisms to convert biochemical energy from nutrients into ATP
Term
Metabolic Pathways
Definition
A series of chemical reactions occurring within a cell
Term
barium hydroxide
Definition
A white powder that reacts with Co2 to produce barium
Term
Cobalt Chloride
Definition
Used to determine absorbance spectrum
Term
guaiacol
Definition
Turns brown when oxidized. Used to measure the rate of breakdown in hydrogen peroxide
Term
phenolphthalein
Definition
A color indicator used in acid testing. It turns pink when put in basic solution
Term
methylene blue
Definition
Stains cells to show nucleus
Term
Sudan IV
Definition
Shows presence of Lipids and fatty acids7
Term
Hydrogen Peroxide
Definition
Substrate that breaks down H2O
Term
Potassium Hydroxide
Definition
Reacts with Co2 to produce a powder
Term
Hydroxylamine
Definition
A competitive inhibitor that competes for the active site.
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