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Bio I Lab - Final Review
Meiosis, DNA, Gene Expression, CSI & Population Genetics
72
Biology
Undergraduate 1
11/25/2013

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Term
DNA isolation
Definition
A process that removes DNA from the confines of a cell.
Term
Lysed cell
Definition
A cell that has had its cell membrane broken down.
Term
Sodium dodecylsulfate (SDS)
Definition
A detergent used to remove the lipids present in the cell membrane so that the DNA can be released.
Term
Polymerase Chain Reaction (PCR)
Definition
A process that amplifies DNA exponentially.
Term
Gel electrophoresis
Definition
Process used to separate DNA fragments based on size and charge.
Term
Anode
Definition
Positively charged portion of gel electrophoresis that attracts the negatively charged DNA molecules.
Term
Visible, plastic, latent, patent, and exemplar.
Definition
The five categories of finger prints.
Term
Plastic
Definition
A category of finger prints categorized by ridge impressions left behind in a soft substance such as soap or wax.
Term
Latent
Definition
A category of finger prints that are left behind on most objects that have been touched. They are invisible to the naked eye and require the use of black powder for identification.
Term
Exemplar
Definition
Category of finger prints that are collected purposefully from an individual.
Term
Patent
Definition
A category of fingerprints characterized by chance ridge impressions, which are visible to the naked eye, imprinted on the surface of a foreign material like wet clay. Typically photographed.
Term
Visible
Definition
A category of finger prints that can be seen by the naked eye and are usually formed when fingerprint ridges come in contact with a colored material.
Term
Loop fingerprint
Definition
A type of fingerprint seen in 60-65% of the general population. One or more ridges must enter from one side of the fingerprint before looping or curving around to exit from the same side.
Term
Ulnar loop
Definition
One of the two major types of loop fingerprints; determined if the loop pattern flow is on the same side as the pinky finger.
Term
Radial loop
Definition
One of the types of loop fingerprints; determined if the loop pattern is on the same side as the thumb.
Term
Whorl pattern
Definition
A fingerprint type seen in 30-35% of the population. The ridges are normally rounded and form a complete circle.
Term
Arch fingerprint
Definition
A type of finger print that is seen in less than 5% of the population. One or more ridges enter from one side and arch before exiting from the opposite side.
Term
Kastel-Meyer test (KM)
Definition
Commonly used test that detects the presence of blood. Also known as the Phenolphthalein assay.
Term
Transfer
Definition
A blood spatter type that happens when a wet, bloody surface, such as a hand or shoe comes into contact with another surface.
Term
Projected
Definition
A type of blood spatter that is created when an exposed blood source is subjected to an action or force greater than the force of gravity.
Term
Arterial spurt
Definition
A type of projected blood spatter characterized by an injury that results in a breached artery releasing blood with every heart beat.
Term
Impact spatter
Definition
A type of projected blood spatter characterized by a massive force or blow resulting in the random dispersion of blood droplets. Further subdivided into: low, medium, and high velocity.
Term
Passive
Definition
A type of blood spatter that consists of blood drops created or formed by the force of gravity alone.
Term
Cuticle
Definition
Outside covering of the hair shaft.
Term
Cortex
Definition
The layer of hair located immediately beneath the cuticle; responsible for hair color.
Term
Medulla
Definition
The inner most layer of the hair shaft. Three types: (1) continuous, (2) interrupted, and (3) absent.
Term
Root
Definition
The terminal end of the hair located within the hair follicle; contains the cells necessary for hair growth.
Term
Natural fibers
Definition
Fibers derived from animal or plant sources. Examples include cashmere, wool, fur, and cotton.
Term
Manufactured fibers
Definition
Fibers derived from either natural or synthetic polymers. Examples include polyester, acrylic, rayon, spandex, and nylon.
Term
Population
Definition
A group of individuals of one species that occupy a defined geographical area and share genes through interbreeding.
Term
Isolation by distance (IBD)
Definition
A mechanism where sub-populations become geographically isolated causing genetic differentiation.
Term
Local population (demes)
Definition
Groups created through IBD that consist of members that are likelier to breed with each other than with the remainder of the population; may eventually evolve into a new species.
Term
Geographic isolation
Definition
A type of prezygotic isolation mechanism where species occur in different areas, which are often separated by a physical barrier such as a river or mountain range.
Term
Ecological isolation
Definition
A type pf prezygotic isolating mechanism where species occur in the same area but they occupy different habitats. Survival of hybrids is low because they are not adapted to either environment of their parents.
Term
Temporal isolation
Definition
A type of prezygotic isolating mechanism where species reproduce in different seasons or at different times of the day.
Term
Behavioral isolation
Definition
A type of prezygotic isolating mechanism where species differ in their mating rituals.
Term
Hardy-Weinberg equation
Definition
An equation that predicts equilibrium if certain conditions are met: (1) large population size, (2) Random mating, (3) No mutation, (4) No migration, and (5) No natural selection.
Term
Mutation
Definition
Evolutionary factor characterized by a change in DNA sequence; the ultimate source of genetic variation. Happens rarely.
Term
Immigration
Definition
Evolutionary factor characterized by the movement of individuals either into or out of a population.
Term
Genetic drift
Definition
A random event that causes diversity within a population. Two examples are (1) founder effect (2) genetic bottlenecks.
Term
Seminiferous tubules
Definition
Location where sperm is produced in the testes.
Term
Spermatogonia
Definition
Parent sperm cells that constantly replicate mitotically throughout a male's life cycle.
Term
Primary spermatocytes
Definition
Spermatogonia that have moved inward towards the lumen of the tubule to begin meiosis.
Term
Secondary spermatocyte
Definition
Two are created through meiosis I of a primary spermatocyte, each with a haploid set of chromosomes.
Term
Spermatids
Definition
Created through meiosis II of secondary spermatocytes.
Term
Sperm cells
Definition
Spermatids that have matured and differentiated.
Term
Oogonia
Definition
Female gamete cells that are produced during early fetal development which reproduce mitotically.
Term
Primary oocytes
Definition
Produced when oogonia reproduce mitotically.
Term
Follicle
Definition
Location where, at puberty, primary oocytes are stimulated by circulating hormones to grow each month.
Term
Graafian follicle
Definition
Produced just before ovulation when the oocyte completes meiosis I; contains the haploid secondary oocyte.
Term
Genotype
Definition
The combination of alleles present.
Term
Phenotype
Definition
Physical expression of the genotype.
Term
Incomplete dominance
Definition
Phenotypic expression that combines the two allelic traits; intermediate between the two homozygotes.
Term
Codominance
Definition
Both phenotypes are expressed at the same time in the heterozygous condition.
Term
Benedict's reagant
Definition
Test that can be used to identify the presence of reducing sugars.
Blue = Negative result
Red = Positive result
Term
Iodine Test
Definition
Test that can be used to detect the presence of the polysaccharide starch.
Black = Positive (starch present)
Yellow = Negative
Term
Biuret reagant
Definition
Test used to identify the presence of the peptide bonds in proteins.
Violet = Positive result
Term
Sudan IV test
Definition
Test used to determine the presence of lipids.
Bright red color/not diffused = Positive result
Red color diffused = Negative result
Term
Dische Diphenylamine Test
Definition
Test used to determine the presence of DNA.
Blue color = Positive result
Term
Competent cells
Definition
Cell with the ability to uptake DNA.
Term
CaCl2 solution
Definition
Solution used to enhance the uptake of plasmid DNA.
Term
Purines
Definition
Double ring structure (A/G)
Term
Pyrimidines
Definition
Single ring structure (T/C)
Term
Prophase I
Definition
Stage in meiosis where crossing over occurs and there is a breakdown of the cell's nuclear envelope.
Term
Metaphase I
Definition
Interlocked homologous chromosomes line up at the middle of the cell; chromosomes are oriented randomly.
Term
Anaphase I
Definition
Homologous chromosomes segregate randomly and independently from each other as they move to opposite poles.
Term
Telophase I
Definition
Stage where the nuclear envelope reforms and two haploid daughter cells are formed at the end of the cytokinesis event.
Term
Interkinesis
Definition
Gap period between Meiosis I and Meiosis II.
Term
Prophase II
Definition
Nuclear envelope breaks down and spindle fibers are formed.
Term
Metaphase II
Definition
Chromosomes align themselves with their sister chromatids oriented toward opposite poles.
Term
Anaphase II
Definition
Phase signaled by the separation of sister chromatids toward opposite poles.
Term
Telophase II
Definition
Sister chromatids have reached opposite poles and the nuclei begin to reform. The second cytokinesis finishes at this time resulting in four haploid daughter cells, each with a single copy of the chromosome.
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