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bio exam chpt 5-8
bio exam chpt 5-8
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Biology
Undergraduate 2
03/28/2010

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Term
hypertonic
Definition
a solution wuth a higher solute concentration; cell less salty than surrounding solution; water moves out of the cell
Term
hypotonic
Definition
a solution with a solute (ex. salt) concentration lower than that of the cell; cell is saltier than surrounding solution;water moves into cell
Term
isotonic
Definition
 the solute concentration of a cell and its isotonic enviornement are essentially equal and the cell gains water at the same rate that it loses it; the cell's volume remains constant
Term
turgid
Definition
plant cell in a hypotonic enviornment; normal for a plant cell; cell turns firm
Term
flaccid
Definition
in isotonic solutions plant cells turn very limp
Term
plasmolyzed
Definition
in a hypertonic enviornment plant cells loses water and shrivel and plasma membrane pulls away from the cell wall; can cause plant to wilt and die
Term
hemolysis
Definition
an animal cell placed in a hypotonic solution gains water and swells and may lyse because there is no cell wall.
Term
crenation
Definition
the contraction of an animal cell after exposure to a hypertonic solution; cell shrivels
Term
ATP
Definition
transfers energy from chemical bonds to endergonic (energy absorbing) reactions within the cell;  multifunctional nucleotide; ATP transports chemical energy within cells for metabolism. It is produced by photophosphorylation and cellular respiration and used by enzymes and structural proteins in many cellular processes.One molecule of ATP contains three phosphate groups, and it is produced by ATP synthase from inorganic phosphate and adenosine diphosphate (ADP)
Term

What happens to phosphate groups in conversion of ATP to ADP

Definition
energy from released in exergonic reactions such as  the break down og glucose during cellular respiration is used to regenerate ATP from ADP. In this endergonic (energy storing) process a phosphate is bonded to ADP. bonds between phosphate group's of ATP's tail can be broken by hydrolysis. energy is releases from ATP when the terminal phosphate bond is broken creating ADP and energy
Term
Osmosis
Definition
the diffusion of water across a selectively permeable membrane from a region of higher water concentration to a region of lower concentration
Term
diffusion
Definition
any molecule moving down its concentration gradient until dynamic equallibrium is reached;the movement of molecules from an area of higher concentration to an area of lower concentration
Term
concentration gradient
Definition
an increase/decrease in the density of a chemical substance in an area
Term
passive transport
Definition
diffusion across a membrane with no energry investment ex. osmosis and diffusion
Term
active transport
Definition
a mechanism for moving a solute agianst its concentration gradient; requires the expenditure of energy in the form of ATP; the mechanism alters the shape of the membrane protien through phosphorylation using ATP
Term
Enzymes
Definition
protiens that function as biological catalysts, increasing the rate of a reaction with being consumed by the reaction. each enzyme has a particular target molecule called the substrate. enzymes are very specific to their substrate
Term
cell membrane
Definition
composed of phospholipids and proteins; most functions of a cell membrane are carried out by the protiens. they are selectively permeable; nonpolar molecules (carbon and oxygen) cross easily, polar molecules (glucose and other sugars) do not cross easily. 
Term
fluid mosaic
Definition
the surface of a cell membrane appears mosaic because of the protiens embedded in the phospholipids and fluid because the protiens can drift about in the phospholipids
Term

 Signal transduction

Definition
relaying message from a membrane receptor to a molecule that preforms a specific function within a cell; a receptor protein has a shape that fits a specfic messenger, such as a hormone. often the binding of the messenger to the receptor triggers a chain reaction involving other protiens which relay the messages to molecules that preform specific functions inside the cell.
Term
ATP synthase
Definition
a cluster of several membrane proteins in the mitochondria that function in chemiosmosis wth adjacent electron transport chains, using the energy of a hydrogen ion concentrantion gradient to make ATP
Term
ATP synthase how does it work?
Definition
ATP synthase act like mini turgbines. the hydrogen ions tend to be driven across the membrane by the energy of their concentration gradient, however the membrane is not permeable to hydrogen ions, and they can only cross through a channel in the ATP synthase.hydrogen ions rush from the intermembrane space into the rotor, causing it to spin,just as water turns turbines in a dam.the hydrogen ions make a nearly full rotation before going into the stator and being released into the mitochondrial matrix. the turning of the rotor causes the internal rod to rotate that activate catalytic sites in the synthase that attach phosphate groups to ADP molecules to generate ATP.
Term
slow twitch muscle fibers
Definition
more efficient at using oxygen to generate more ATP; allow continuous extended muscle contrations over a long time; they fire more slowly than fast twitch fibers and can go for a long time before the fatigue.
Term
fast twitch muscle fibers
Definition
use anaerobic metabolism to create fuel; much better at generating short bursts of strength or speed than slow twitch fibers; fatigue more quickly. fast twitch fibers generally produce the same amount of force per contration as slow muscles, but they get their name because they are able to fire more rapidly; not effective in longer-term train but useful in brief, high-intensity training like sprinting, body building or powerlifting
Term
cellular respiration
Definition

a process that releases energy from bonds in glucose and captures the energy as ATP; controlled break down of organic molecules

C6H12O6(glucose)+6O2à6CO2+6H2O+ATPs

Term
Overall  purpose of cellular respiration?
Definition
produce ATP (38 ATPs to be exact)
Term
fermentation
Definition
occurs is there is not enough oxygen to undergo cellular respiration; plan B for our cells and used as a last resport becuase it is less effiecent at producing ATP (only produces 2 where as respiration produces 38); anaerobic energy-generating process; takes advatange of gycolysis, producing 2 ATP molecules and reducing NAD+ to NADH 
Term
anaerobic
Definition
without oxygen
Term

Will a yeast use fermentation or respiration in the presence of oxygen?

Definition
respiration
Term
yeast
Definition
yeast are single-celled fungi that not only use respiration of energy but can ferment under anaerobic conditions; they convert pyruvate to CO2 and ethanol while oxidizing NADH back to NAD+.
Term
alcohol fermentation
Definition

2 ATP produced

1glucose +2NAD-> 2ethanol + 2CO2 + 2ATP + 2NADH

 

Term
lactic acid fermentation
Definition

in your muscle cells

 1glucose +2NAD-> 2lactate + 2ATP + 2NADH

Term
glycolysis
Definition

occurs int he cytoplasmic fluid of the cell, that is, outisde the organelles. breaks down glucose into two molecules of a 3 carbon compound called pyruvate; 2 net ATP produced

glucose --> 2 pyruvate + 2 ATP + 2 NADH

Term
citric acid cycle
Definition

krebs cycle in animals;takes place in the mitochondria; completes the break down of glucose by decomposing a derivative of pyruvate to carbon dioxide.

1acetyl CoA + 10 Oxaloacatate --> 1 Oxaloacatate + 1ATP + 3NADH + 1FADH + 2CO2

Term
main function of gycolysis and the citric acid cylce?
Definition
supple the 3rd stage of repiration with electrons
Term
between glycolysis and the citric acid cycle
Definition

pyruvate is transported to the mitochondria where it is perpared for entry into the citric acid  cycle.

pyruvate --> acetyl CoA(coenzyme A) + CO2

Term
oxidative phosphorylation
Definition
where most of the ATP produced by cellular respiration is generated; involves the electron transport chain and chemiomosis. NADH and a related electron carrier FADH2 shuttle electrons to the electron transport chain embedded in the inner mitchondrion membrane; uses energy released by the downhill fall of electrons from NADH and FADH2 to O2 to phosphorylate ADP. as each electron is passes from protien complex to protein complex it loses energy; each electron steps down an energy stair with each passage until it reaches its final destination and is accpeted by oxygen; oxygen will simultaneously accept the electrons and the H+ ions to form water
Term
electron transport chain
Definition
in the cristae of the mitchondria; the carriers accept and donate electrons; electrons are finally passed to O2 forming H2O; the electron transport chain generates no ATP; the chains function is to break the release of energy into smaller steps that release energy in manageable amounts
Term
NADH and FADH2
Definition

electron carriers; donate electrons to the electron transport chain which powers ATP synthesis (oxidative phosphorylation); electrons in

NADH and FADH contain potential energy (this energy is used to pump H+ ions across the membrane) 

Term
chemiosmosis
Definition
energy-coupling mechanism; the use of energy in a H+ gradient to drive cellular work. EX. electron transfer in the electron transport chain causes proteins to pump H+ from the mitchodrial matrix to the interspace membrane, H+ the moves back across the membrane passing through channels in the ATP synthase, ATP synthases uses exergonic flow of H+ to drive phosphorylation of ATP
Term
redox reaction
Definition

movement of electrons from one molecule to another

[image]

Term
oxidation
Definition
in a redox reaction the loss of electrons from one substance
Term
reduction
Definition
in a redox reaction the addition of electrons to one substance
Term
autotrophs
Definition
living things that are able to make their own food with out using organic molecules derived from any other living thing; producers of the biosphere
Term
photoautotrophs
Definition
autotrophs that use the energy of light to produce organic molecules; plants algea and protists and some prokaryotes
Term
chemoautotrophs
Definition
synthesize all necessary organic compounds from heat methane and sulfur; live in hostile enviornment like deep sea vents
Term
chloroplast
Definition
major sites of photosynthesis in green plants; organelles consisting of photosynthetic pigments, enzymes, and other molecules grouped together; the ability to synthesize is directly related to the structure of chloroplasts
Term
chlorophyll
Definition
an important light absorbing pigment in chloroplast; responsible for the green color of plants; energy provided by light is primarily absorbed by chlorophylls and carotenoids;
Term

Best color of light for photosynthesis

Definition

chlorophylls absorb blue and red light and carotenoids absorb blue-green light; green and yellow are not effectively absorbed by photosynthetic pigments in plants, therefore the light of these colors is either reflected by leaves or passes through the leaves; this is why plants are green.

the best light is red or blue

Term
photosynthesis
Definition

Transformation of light energy to chemical energy stored in the bonds of sugar they make from carbon dioxide and water; produces oxygen.

Electrons gain energy by being boosted up an energy hill; light energy captured by chlorophyll molecules provides the boost of energy for the electrons

Like cellular respirartion photosynthesis is a redox reaction; water molecules are split apart by oxidation which means they lose electrons along with hydrogen ions; then CO2 is reduced to sugar as electrons and hyrdrogen ions are added to it

6CO2 + 6H2O --(light energy)--> C6H12O6 + 6O2

Term
stomata
Definition
tiny pores in the leaf that allow carbon dioxide to enter and oxygen to exit
Term
light reactions
Definition

Light energy is converted in the thylakoid membranes to chemical energy and O2; water is  split to provide the O2 and electrons

Chemiosmosis power ATP synthesis in the light reactions.

2 connected photosystems;H+ ions reduce NADP+ TO NADPH, which is an electron carrier similiar to NADH; NADPH is temorarily stored the shuttled into the Calvin cycle where it is used to make sugar; the light reactions generate ATP

NADP++ H2O + ADP+P -(light)--> O2 + ATP +NADPH

Term
photosystems
Definition

2 within the light reaction connected by an electron transport chain; generates ATP and NADPH

Steps:

1. a photon of light is absorb by chlorophyll

2.energy from light causes electron to jump to a higher energy state

3. electron transport chain produces H+ gradient and ATP

4. light absorbed by another chlorophyll

5. energy from light causes electron to jump to a higher energy state

6. electron is donated to NADP+

Term
calvin cycle
Definition

kreb cycle in plants;converts CO2 into sugars; makes sugar within a chloroplast; using CO2, and the ATP and NADPH generated in the light reactions, an energy rich  3 carbon sugar called gyceraldehyde-3-phosphate (G3P) is produced.

CO2 + ATP + NADPH --> Glucose

Term
reactive oxygen species (ROS)
Definition
ions or molecules that are highly reactive do to the presence of unpaired valence shell electrons; form as a natural byproduct of cellular respiration; can damage cells
Term
antioxidants
Definition
help fight ROS; lower the risk of heart disease and neuological disease; protect agianst cancers; one class of antioxidants are carotenoids found in fruits and veggies
Term
Nondisjunction
Definition

an accident of meiosis in which a pair of homologous chromosomes or a pair of sister chromatids fail to seperate at anaphase.

-if it occurs in during meiosis I then both members of a homologous pair go to one pole 

-if it occurs in during meiosis II then both sister chromatids go to one pole

Fertalization after nondisjunction yeilds zygotes with altered #s of chromosomes

Term
cell cycle
Definition
ordered sequence of events for cell division; consist of interphase and mitotic phase
Term
interphase
Definition

duplication of cell contents including DNA; mostly time for growth and DNA synthesis

1. G1- growth, increase in cytoplasm

2. S- DNA synthesis, duplication of chromosomes

3. G2- growth, prep for cell division

the end of interphase there are 42 chromosomes and 92 chromatids

Term
mitotic phase
Definition
cell division; mitosis- division of the nucleus; cytokinesis- division of cytoplasm
Term
mitosis
Definition
- division of a cell to produce 2 identical cells; used mainly for growth and replacement
Term
meiosis
Definition
division of cells to produce 4 daughter cells (gametes) with a single set of chromosomes
Term
asexual reproduction
Definition
offspring are identical to the original cell/organism;involves inheritance of all genes from one parent
Term
sexual reproduction
Definition
offspring are similar to parents, but show variations in traits; involves inheritance of unique sets of genes from 2 parents
Term
genome
Definition
all the DNA in a cell
Term
chromosomes
Definition
a thread-like, gene-carrying structure found in the nucleus of eukaryotic cells and most visible during mitosis and meiosis; main gene carrying structure of a prokaryotic cell; consists of DNA and protien; every eukaryotic species has a characterist # of chromosomes in each nucleus; 23 chromosomes at the end of interphase
Term
somatic cells
Definition
nonreproductive; have pairs of homologous chromosomes; they are diploid (46 in humans)
Term
gametes
Definition
reproductive cells (sperm and egg); have half as many chromosomes as somatic cells; they are haploids (23 in humans)
Term
chromatin
Definition
chromosomes as a mass of thin long fibers; combo of DNA and protien; as the cell prepares to divide its chromatin coils up forming compact, distinct chromosomes; 46 at the end of interphase
Term
centromere
Definition
a narrow "waist" where 2 chromatids are tightly joined
Term
prophase
Definition

in the nucleus; chromosomes coil and become compact;

the end of prophase there are 46 chromosomes and 92 chromatids

Term
metaphase
Definition

chromosomes align at the cell equator; mitotic spindle is fully formed with poles at the opposite ends of the cell;

the end of metaphase there are 46 chromosomes and 92 chromatids

Term
anaphase
Definition
sister chromatid seperate at the centromeres; daughter chromosomes are moved to opposite poles of the cell; the end of anaphase there are 92 chromosomes and 92 chromatids
Term
telophase
Definition

the cell continues to elongate; the nuclear envelope forms around chromosomes at each pole, establishing daughter nuclei;chromtin uncoils;

the end of telophase there are 92 chromosomes and 92 chromatids

Term
cytokinesis
Definition
cytoplasm is divided into seperate cells
Term
homologous chromosomes
Definition
chromosomes matched in length, centromere position, and gene locations; seperation during meiosis can lead to genetic differences between gametes; different versions of a gene at the same locus (position of a gene), one version inherited from the maternal parent and the other from the paternal parent; do not end up in the same cell at the end of meiosis so, gametes will receive either the maternal or paternal version of the gene
Term
zygote
Definition
diploid chromosome # one set from each parent
Term
prophase I
Definition
chromosomes become compact; homologous chromosomes come together as pairs by synapsis;each pair w/ 4 chromatids is called a tetrad; nonsister chromatids exchange genetic material by crossing over
Term
crossing over
Definition

causes genetic recombination (production of new combos of genes);involves exchange of genetic material between homologous chromosomes

- nonsister chromatids join at the chiasma (the site of attachment and crossing over)

-corresponding amounts of genetic material are exchanged between maternal and paternal chromatids

Term
metaphase I
Definition
terads align at the cell equator
Term
anaphase I
Definition
homologous pairs seperate and move towards opposite poles of the cell
Term
telophase I
Definition
duplicated chromosomes have reached the poles; a nuclear envelope forms around the chromosomes in some species; each nucleus has the hapoid # of chromosomes
Term
meiosis II
Definition

follows meiosis I without chromosome duplication;Each of the two haploid products enters meiosis II

 Prophase II-Chromosomes coil and become compact

Metaphase II-Duplicated chromosomes align at the cell equator

Anaphase II-Sister chromatids separate and chromosomes move toward opposite poles

Telophase II -Chromosomes have reached the poles of the cell;A nuclear envelope forms around each set of chromosomes; With cytokinesis, four haploid cells are produced

 

Term
which characteristics are similar for mitosis and meiosis?
Definition
one duplication of chromosomes
Term
which characteristics are unique to meiosis?
Definition

2 divisions of chromosomes(meiosis I and meiosis II

Occurs to create haploid gametes);Pairing of homologous chromosomes for crossing over and across the metaphase plate in metaphase I; Exchanging of genetic material by crossing over

Term
cancer cells
Definition
escape controls of the cell cycle; cancer cells divide rapidly, often in the absence of growth factors; they spread to other tissues through the circulatory system; growth is not inhibited by other cells and tumors form
Term
density dependent inhibition
Definition
the arrest of cell division that occurs when cells grown in a lab dish touch one another
Term
trisomy 21
Definition
causes down syndrome;involves inheritance of 3 copies of chromosome 21; most common humna chromosome abnormality;chromosome 21 is one of the smallest human chromosomes, this provides an explanation as to why this condition is not fatal while trisomy involving another larger autosome that controls many more genes would most likely be fatal
Term
down syndrome
Definition

-caused by an imbalance in chromosome number; characterized by characteristic facial features, susceptibility to disease, shortened life span (40-50 years), mental retardation, variation in characteristics.

-The probability of concieving a baby with down syndrome increases with the age of the mother; age 20-24 probability is 1/1562, 35-39 probability is 1/214, older than 45 the probability is 1/19

Term
tissue plasminogen activator (TPA)
Definition
an enzyme, that catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown.Because it works on the clotting system, tPA is used in clinical medicine to treat only embolic or thrombolytic stroke. Use is not recommended in hemorrhagic stroke and head trauma.
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