Shared Flashcard Set

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Bio 1208 Final
Final flashcards
9
Biology
Undergraduate 1
12/03/2013

Additional Biology Flashcards

 


 

Cards

Term
o Mechanical or micropipette
Definition
• Used for small volume transfers where a high degree of precision is required
• Volume set by turning the volume adjustment rings
• Draw up to the exact amount needed to be transferred
below 1 ml
Term
o Volumetric pipet
Definition
• Designed to deliver a single volume precisely
• The volume is indicated near the top of the pipet
5 ml
Term
o Mohr Pipet
Definition
• Graduated but stop at a baseline before the pipet begins to narrow
10 ml
Term
o Serological Pipet
Definition
• Graduated to deliver with no base mark
• The appropriate amount of fluid is drawn into the pipet and the entire amount is transferred
• Labeled at the top with a zero
• Subtract to know proper amount
10 ml
Term
o Pi-Pump
Definition
• Device used to safely and easily draw fluid into the pipet
Term
o Spectrophotometer
Definition
detects the amount of radiant energy absorbed by the molecules.
o Light absorbed is proportional to concentration
Term
Spectrophotometer
Definition
o Turn on and let warm up for 15, set knob on top to appropriate wavelength, set left handed knob to 0, put in blank and set mode to transmittance, turn right knob to 100%, switch out blank to molecule tube and set mode to absorbance.
Term
o Paper chromatography-
Definition
a technique used to separate complex mixtures into its component parts so individual pigments can be observed.
Term
o How it works-it separates complex mixtures (like photosynthetic pigments) into its component parts. Chromatographic systems consist of a mobile and stationary phase. Molecules with a strong attraction for the mobile phase move with the mobile phase while molecules with a strong attraction to the stationary phase remain still during the mobile phase.
Definition
Paper Chromatography
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