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Bio 101 Lecture 4: Mitosis
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Biology
Undergraduate 2
10/06/2012

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Term
Mitosis
Definition
Type of cell division that results in two daughter cells
-Each the same as the parent nucleus
Term
Diploid Cell (2n)
Definition

-Somatic Cells -Contains both members of each pair of homologous chromosomes

 

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Term
Haploid Cells (1n)
Definition

Contains only one member of each homologous chromosome pair

 

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Term
(Terminally) Differentiated Cell
Definition
The normal process by which a less specialized cell develops or matures to possess a more distinct form and function.
-Red blood cell: loses its nucleus in order to carry oxygen
Term
Unicellular Organisms
Definition
Reproduce asexually
Term
Somatic Cells
Definition
-Any cell in the body of an organism/multicellular organisms.
-There is growth/development and repair/renewal in cells
Term
Eukaryotic Cell: Genetic Material
Definition

DNA + Histones = Nucleosome = become coiled i= Chromatin. Chromatin coils into Chromosomes. Chromosomes are in the nucleus of a cell.

 

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Term
Nucleolus Structure
Definition

-10nm in diameter -DNA winds around a cluster of histone molecules -Linker DNA between histone molecules -Histone Tails present

 

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Term
Organization of Eukaryotic Chromosome
Definition

Different levels. Nucleosome: 1st level. Chromatin formed in: 2nd level. Fibers looping: 3rd level.

 

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Term
Cell-Division Cycle
Definition
The series of events that takes place in a cell leading to its division and duplication (replication). Eukaryotic unique only, with nucleus present.

1) INTERPHASE- G1, S, G2.
2) M PHASE- Mitosis, Cytokinesis.
Term
Interphase
Definition
The longest part of the cycle (23 hours in mammalian species. Phase where cell gets ready to divide Consists of:
G1, S, G2.
Term
G1 Phase
Definition
-The first growth phase in interphase
-Cell synthesizes proteins and produces cytoplasmic organelles..
Term
S Phase
Definition
-Synthesis phase
-Main: DNA replication
-Histone proteins are synthesized as well so that the cell can make duplicate copies of its chromosomes.
Term
G2 Phase
Definition
-Shorter in comparison to G1 and S phase
-Increased protein synthesis occurs,
-Final steps in the cell’s preparation for division
-Right before mitosis/as it initiates, centrosomes move to opposite ends
Term
M Phase
Definition
-10% of cell division cycle
-Consists of mitosis and interphase
Term
Prophase
Definition
-Mitotic spindles begin to form
-Nuclear envelope breaks up
-Chromatin begins to foil into chromatin, which are split into two sister chromatids
-Kinetochores attach and form to centromeres
Term
Centromere
Definition
Part of the chromosome that links two sister chromatids together
Term
Kinetochores
Definition
-Protein structues attached to each centromere
-Microtubules bind to them
Term
Metaphase
Definition
-Spindle microtubules attach to the kinetochores of chromosomes/chromatids, -Attach chromosomes to the cell poles
-Line chromosomes up at equatorial/metaphase plate
-Nuclear envelope disappears
Term
Anaphase
Definition
-Spindle poles move farther apart and the spindles pull the sister chromatids apart at their centromeres. Each daughter chromosome pulled towards one end.
Term
Centrioles
Definition
They are within the centrosomes, and as the centrosomes split, a pair of centrioles are at each spindle pole
-Spindle microtubules come out of centrioles
Term
Telophase
Definition
-Chromosomes grouped at poles
-Nuclear envelope reforms
-Cleavage furrow
Term
Cytokenisis
Definition
-Cytoplasm pinching leads to a forming of a cleavage furrow (pinched by microfilaments as a ring contracts)
-The cytoplasm splits on the furrow, forming two daughter identical daughter cells with nuclei each
Term
Prometaphase
Definition
In between prophase and metaphase
When spindles shoot out and chromosomes are being pulled by spindles, but not yet lined in middle
Term
Cell Plate
Definition
-Forms during plant cell "cytokinesis"
-How cells duplicate in plant cells, instead of cleavage furrow
Term
Cell Cycle Checkpoints (and examples)
Definition
Requirements for cell division phases to go on.
G1 --> S2. Is cell big enough? No? Stop. Is there DNA damage? If yes, stop.
G2 --> M: Is DNA fully replicated? If no, stop. Is there DNA damage? If yes, stop.
Metaphase to Anaphase: Are all sister chromatids attached? If no, stops.
Also: from G1 ---> S/G2 ---> M, depends on activation of a protein cyclin-dependent kinase (CDK)
Term
Binary Fission (Steps)
Definition
-DNA Replication begins at single site on bacteria chromosome
-Replication enzymes work in opposite directions on chromosome
-Daughter chromosomes separate, and Z ring forms at cell. Cell wall appears
-Two identical daughter cells
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