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Bio 101
Test 3
110
Biology
Undergraduate 1
11/03/2011

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Term
Cell cycle
Definition
the series of events that take place in a cell leading to its division and duplication

takes about 24 hours
Term
Interphase
Definition
the portion of the cell cycle between one mitotic phase and the next

includes the G1, S, and G2 phases

involves growth, preparation for division, or the fulfillment of specialized functions in the organism
Term
G1 (gap 1) phase
Definition
the phase of the cell cycle that constitutes the first part of interphase before DNA synthesis and occurs after the mitotic phase

takes about 7-9 hours
Term
S (synthesis) phase
Definition
the phase of the cell cycle during which DNA is synthesized and chromosomes are replicated

takes about 6-8 hours
Term
G2 (gap 2) phase
Definition
the phase of the cell cycle between synthesis of DNA and mitosis

the last stage of interphase

takes about 6-8 hours
Term
Mitotic (M) phase
Definition
the phase of the cell cycle during which a parent cell divides to create two genetically identical daughter cells

Mitosis makes up one half of the mitotic phase; cytokinesis makes up the other
Term
Mitosis
Definition
The process of cell division to create two genetically identical daughter cells

includes prophase, metaphase, anapahse, and telophase
Term
Cytokinesis
Definition
Division of the cytoplasm and organelles

one out of two parts of the mitotic phase of the cell cycle
Term
Prophase
Definition
the first stage of mitosis or meiosis

chromosomes condense into compact structures and become visible

spindle apparatus forms
Term
Spindle apparatus
Definition
an array of microtubules responsible for pulling chromosomes to the poles of the cell and for pushing the poles of a cell away from each other during either mitosis or meiosis
Term
Prometaphase
Definition
the nucleolus of the cell disappears and the nuclear envelope disintegrates

kinectechore microtubules from each spindle apparatus attach to one of the two sister chromatids of each chromosome
Term
Metaphse
Definition
a phase in mitosis and meiosis during which the chromosomes line up in the middle of the cell
Term
Anaphase
Definition
a phase in mitosis and meiosis in which chromosomes are moved to the opposite ends of the cell

centromeres holding sister chromatids together split

sister chromatids are pulled apart, creating independent chromosomes

motor proteins pull chromosomes to opposite ends of the cell and push opposing poles of the cell away from each other
Term
Telophase
Definition
the final stage in mitosis or meiosis in which sister chromatids separate and new nuclear envelopes begin to form around the daughter chromosomes
Term
Somatic cell
Definition
Any type of cell in a multicellular organism except eggs, sperms, and their precursor cells

also called body cells
Term
Gamete
Definition
a haploid reproductive cell that can fuse together with another haploid cell to form a zygote

most common: egg and sperm cells
Term
Genome
Definition
all of the hereditary information in an organism
Term
Chromatin
Definition
the complex of DNA and proteins that make up eukaryotic chromosomes
Term
Sister chromatids
Definition
the paired strands of a recently replicated chromosome

connected at the centromere
Term
Centromere
Definition
constricted region of a replicated chromosome where the sister chromatids are joined and the kinectechore is located
Term
Haploid
Definition
having one set of chromosomes (1n)
Term
Diploid
Definition
having two sets of chromosomes (2n)
Term
trait
Definition
any heritable characteristic of an individual
Term
sex chromosome
Definition
chromosome associated with an individual's sex
Term
autosome
Definition
a non-sex chromosome
Term
homologous chromosome
Definition
chromosomes that are similar in size, shape, and gene content

also called homologs
Term
allele
Definition
a particular version of a gene
Term
karyotype
Definition
the particular appearance of all the chromosomes in an individual, including the number of chromosomes and their length and banding patterns
Term
tetrad
Definition
the structure formed by synapsed homologous chromosomes during prophase I of meiosis
Term
Principle of Independent Assortment
Definition
the concept that each pair of hereditary elements behaves independently of other genes during meiosis
Term
phenotype
Definition
the observed effect of a genotype; a visible trate
Term
genotype
Definition
all of the alleles of every gene present in a given individual

may refer specifically to a set of alleles under study
Term
Hypothesis of Blending Inheritance
Definition
offspring will have blended parent phenotypes
Term
Hypothesis of Inheritance of Acquired Characteristics
Definition
through use, traits are modified and passed down to offspring
Term
model organism
Definition
used for research because of practicality, and the conclusions drawn from studies on this organism can be applied to others
Term
self-fertilization
Definition
in plants, the fusion of two gametes from the same individual to form a diploid offspring

also called "selfing"
Term
cross fertilization
Definition
mating that combines gametes from different individuals
Term
pure line
Definition
individuals always have offspring identical to them
Term
hybrid
Definition
offspring of parents from two different strains, populations, or species
Term
F1 generation
Definition
first filial

second generation

offspring of parent generation
Term
F2 generation
Definition
second filial

third generation

offspring of F1 generation
Term
homozygous
Definition
both alleles for a gene are the same

(ie RR or rr)
Term
heterozygous
Definition
the alleles for a gene are different

(ie Rr)
Term
reciprocal cross
Definition
a breeding experiment in which the mother's and father's phenotypes are the reverse of those in the previous experiment

Mendel used reciprocal crosses to study the effects that gender had on heritable traits
Term
Mendel's Law of Segregation
Definition
the two members of each gene pair must separate into different gamete cells during the formation of eggs and sperm in the parents. As a result, each gamete contains one allele of each gene.
Term
Incomplete dominance
Definition
inheritance pattern in which the heterozygote phenotype is a blend or combination of both homozygote phenotypes
Term
multiple allelism
Definition
the existence of more than two alleles of the same gene (normal in some species)
Term
pleiotropic genes
Definition
a gene that can affect more than one phenotypic trait

causes Marphan's syndrom
Term
Chromosome Theory of Inheritance
Definition
basic principle in biology that states that genes are on the chromosomes and that the behavior of chromosomes during meiosis accounts for inheritance pattersn
Term
nuclotide
Definition
a molecule onsisting of a five-carbon sugar (ribose or deoxyribos), a phosphate group, and one of several nitrogen-containing bases.

DNA and RNA are polymers of nucleotides containing deoxyribose and ribose, respectively
Term
DNA's primary structure
Definition
consists of the sugar-phosphate backbone and the sequence of nitrogenous bases
Term
DNA's secondary structure
Definition
consists of hydrogen bonding between the nitrogenous bases

otherwise known as complementary base pairing
Term
RNA's primary structure
Definition
consists of the sugar phosphate backbone and the sequence of nitrogenous bases
Term
RNA's secondary structure
Definition
consists of hydrogen bonding between nitrogenous bases on the same strand of RNA
Term
RNA's tertiary structure
Definition
occurs because of interactions that fold the RNA molecule
Term
RNA's quarternary structure
Definition
occurs because of interactions that hold different strands of RNA together
Term
pyrimidines
Definition
single-ringed nitrogenous bases including cytosine, uracil, and thymine

pair only with purines
Term
purines
Definition
double-ringed nitrogenous bases including guanine and adenine

pair only with pyrimidines
Term
nucleotide polymerization
Definition
dNTPs (three phosphate groups)are added to nucleotide polymers in polymerization

phosphate group is linked to sugar

other two phosphates are released as pyrophosphates, which will eventually be hydrolyzed into inorganic phosphates

phosphodiester bonds are formed between the phosphate group of one nucleotide to the sugar of the one next to it
Term
points of origin
(in DNA replication)
Definition
special sites where replication begins

proteins that initiate DNA replication recognize points of origin and bind, opening the DNA strands to make replication bubbles

prokaryotic cells have one replication bubble and eukaryotic cells have many
Term
DNA topoisomerase
Definition
enzyme that relaxes double helix
Term
helicase
Definition
enzyme that separates strands and creates replication forks
Term
single-strand DNA binding protins (SSBPs)
Definition
proteins that stabilize/prevent double helix from reforming during replication
Term
DNA polymerase
Definition
add complementary base pairs to each separated strand

cannot initiate without primers
Term
primers
Definition
short RNA strands that attach at specific points
Term
dNTPs
Definition
source of energy-replication because of its three phosphate groups (like in ATP)
Term
semiconservative molecule
Definition
one strand of each new DNA molecule is from original DNA molecule (one strand is old, one is new)
Term
DNA polymerase III
Definition
recognizes errors of replication and fixes; "proofreads" replication
Term
central dogma of molecular biology
Definition
long-accepted hypothesis that information in cells flows in one direction: DNA codes for RNA, which codes for proteins

exceptions are now known
Term
transcription
Definition
the process by which RNA is made from a DNA template

takes place in the nucleus
Term
initiation
Definition
the stage during which ribosome orient reactants precisely as they bind at specific locations within the ribosome's active site
Term
elongation
Definition
the process by which messenger RNA lengthens during transciption
Term
termination
Definition
the final stage in which the ribosome returns to its original conformation and products are release
Term
coding strand
Definition
the strand of DNA that is not transcribed during synthesis of RNA

its sequence corresponds to that of the mRNA produced from the other strand
Term
translation
Definition
the nprocess by which proteins and peptides are synthesized from messenger RNA

takes place in the cytoplasm
Term
tRNA
Definition
transfer RNA

one of a class of RNA molecules that have an anticodon at one end and an amino acid binding site at the other

each tRNA picks up a specific amino acid and binds to the corresponding codon in messenger RNA during translation
Term
genetic code
Definition
the set of all 64 codons and the particular amino acid that each specifies
Term
triplet code
Definition
a code in which a "word" of three letters encodes one piece of information

the genetic code is a triplet code because a codon is three nucleotides long and encodes one amino acid
Term
codon
Definition
a sequence of three nucleotides in DNA or RNA that codes for a certain amino acid that initiates or terminates protein synthesis
Term
genetic engineering the manipulation of genes
Definition
the maniplation of gene
Term
restriction endonucleases
Definition
enzymes that "cut up" foreign DNA

hundreds have been discovered, each unique for a certain purpose

usually "cut" DNA palindormes
Term
palindromes
Definition
when the 5'-->3' sequence on one strand is the same as the 5' --> 3' sequence on the antiparallel strand - GAATTC on one and CTTAAG on the other
Term
DNA ligase
Definition
catalyzes formation of phosphodiester bonds
Term
reverse transcriptase
Definition
an enzyme used with the RNA --> DNA process that makes single or double-stranded DNA from RNA
Term
mutation
Definition
any change in the hereditary material or an organism

can either be beneficial, neutral, or deleterious
Term
point mutation
Definition
one nucleotide in a serious is added or changed
Term
silent mutation
Definition
a mutation that results in the same amino acid formation and is therefore ineffective
Term
nonsense mutation
Definition
codes for "stop" codon

proteins stop being engineered before they are complete
Term
missense mutation
Definition
a change in a base pair on DNA that causes the formation of a different amino acid on the polypeptide
Term
frameshift mutation
Definition
the addition or deletion of a nucleotide
Term
polyploidy
Definition
a organism has more than two sets of chromosomes (normal in some species)
Term
aneuploidy
Definition
addition or deletion of one chromosome

occurs because of mistakes in either species
Term
inversion
Definition
a part of a chromosome is flipped, or inverted
Term
translation
Definition
part of a chromosome detaches from one chromosome and attaches to another
Term
karyotype
Definition
method of organizing a species' chromosomes based on size, structure, and number
Term
extremophiles
Definition
bacteria that exist in nextreme environments
Term
pathogenic bacteria
Definition
disease-causing
Term
Germ Theory of Disease
Definition
certain diseases can be passed from organism to organism and are called infectious diseases; certain bacteria and viruses are involved
Term
antibiotics
Definition
any substance that can kill or inhibit the growth of bacteria

overuse of antibiotics leads to bacteria becoming resistant to them
Term
virulence
Definition
ability of a pathogen or parasite to causes disease or death
Term
E. Coli
Definition
generally harmless in gastro-intestinal tract, but some can cause food poisoning by adhering to cells and secreting toxins to harm an organism
Term
opportunistic bacteria
Definition
bacteria that can overcome the body's defenses and cause disease when the body is not as healthy as it can be
Term
exotoxins
Definition
proteins are secreted by the bacterial cell
Term
endotoxins
Definition
components of outer membrane of certain gram-negative bacteris
Term
morphology
Definition
diversity in size, shape, and appearance
Term
phototroph
Definition
"light-feeders"

use light energy to promote electrons to the top of the electron transport chain

ATP is said to be produced by photophosphorylation
Term
chemoorganotrophs
Definition
oxidize organic molecules (ie sugars), which serve as electron donors
Term
chemolithotrophs
Definition
oxidize inorganic molecules, which serve as electron donors
Term
autotroph
Definition
organism that makes its own C-C compounds
Term
heterotroph
Definition
organism that consumes C-C compounds from autotrophs
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