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BILD 3 Lecture 07
Prokaryotes
49
Biology
Undergraduate 1
10/24/2010

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Term
Prokaryotes
Definition
-most are unicellular but some form colonies (cooperation makes them multicellular): ex. gingivitis/dental plaque
-can thrive everywhere
-variety of shapes
-move via flagella
-cell wall
-lack complex compartmentalization
-reproduce by binary fission
Term
Thermophiles
Definition
live at high temperatures
Term
Halophiles
Definition
live in high saline environments
Term
Three Domain System
Definition
Eukarya, Bacteria, Archaea
Term
Amount of genetic diversity among prokaryotes
Definition
far greater than among all Eucarya put together
Term
Horizontal Transfer
Definition
process in which an organism incorporates genetic material from another organism without being the offspring of that organism
Term
Five Kingdoms (how it used to be)
Definition
Monera, Protista, Plantae, Fungi, Animalia
Term
Prokaryotes divided into 2 domains
Definition
bacteria and archaea
Term
Archaea and Eukarya share a more recent common ancestor than do
Definition
Archaea and Bacteria
Term
Prokaryotic Success driven by
Definition
structural, metabolic, and genetic adaptations
Term
Prokaryotic cell size
Definition
smaller than eukaryotic (1/10th to 1/100th)
Term
3 most common shapes of prokaryotic cells
Definition
spheres(cocci), rods(bacilli), and spirals
Term
Prokaryotic behavior
Definition
in a heterogeneous environment, they exhibit taxis or the ability to move toward or away from certain stimuli
Term
Prokaryotic Cell Wall
Definition
maintains cell shape, provides protection, and prevents cell from bursting in hypotonic environment
Term
Bacteria Cell Wall
Definition
peptidoglycan- different from archean and eukaryote cell walls
Term
Gram Stain
Definition
technique that enables scientists to classify bacteria species into two groups: Gram-Positive and Gram-Negative
Term
Gram Positive
Definition
have cell wall with large amount of peptidoglycan that traps the violet dye in the cytoplasm
-violet dye cannot be rinsed from cytoplasm thereby masking the added red dye
Term
Gram Negative
Definition
have less peptidoglycan of which is located in a layer between the plasma membrane and outer membrane
-violet dye can be rinsed from the cytoplasm and thus the cell appears pink or red after red dye is added
Term
Prokaryotes Lack
Definition
membrane bound nucleus, membrane bound organelles, and cytoskeleton
Term
Prokaryotic Genome
Definition
circular ring of DNA - single chromosome
Term
Plasmids
Definition
smaller rings of DNA usually not vital to normal functioning but allow for resistance to antibiotics
Term
Reproduction facilitates…
Definition
adaption
-rapid reproduction, large population sizes, and horizontal gene transfer facilitate the evolution of prokaryotes to changing environments
Term
Phototrophs receive energy from
Definition
the sun
Term
Chemotrphos receive energy from
Definition
chemicals
Term
Autotrophs receive Carbon from
Definition
CO2
Term
Heterotrophs receive carbon from
Definition
other organic chemicals
Term
Chemoautotrophs
Definition
-live in sulfurous hot springs
-use sulfur as energy source
-get carbon from CO2
Term
Deep-sea thermal vents
Definition
-fissures in a planet's surface
-geothermically heated water spews
-rich in minerals
-communities in these thermal vents are often very deep where theres no light and thus rely on chemoautorophic arch for primary production
Term
Obligate aerobes
Definition
require oxygen
Term
Facultative anaerobes
Definition
can survive with or without oxygen
Term
Obligate anaerobes
Definition
are poisoned by oxygen
Term
C. botulinum
Definition
-produces protein that causes disease botulism
-nerve toxin causes musculoskeletal paralysis, very lethal
-lives in canned foods
-killed by high temps
Term
Metabolic Cooperation
Definition
allows prokaryotes to use environmental resources they could not use as individual cells
-in some species this occurs in surface coating colonies called biofilms
Term
Biofilm
Definition
surface coating colonies
Term
If prokaryotes where to disappear
Definition
prospects for any other life surviving would be slim since prokaryotes play crucial roles in the biosphere
Term
Roles of Prokaryotes
Definition
-chemical recycling between living and nonliving components of the environment in ecosystems
-chemoheterotrohpic prokaryotes function as decomposers
-nitrogen-fixing prokaryotes add usable nitrogen to the environment
Term
Symbiotic Relationships
Definition
many prokaryotes live with other organisms with symbiotic relationships such as mutualism and commensalism
Term
Example of symbiosis
Definition
bobtail squid and its light organ
Term
Pathogenic Prokaryotes
Definition
cause half of all human diseases
-ex. lyme disease
-cause disease by releasing exotoxins or endotoxins
Term
Western Fence Lizard and Lyme disease
Definition
lyme disease infected ticks feed on blood of these lizards
-lizards blood destroys the bacteria
Term
Biotechnologist's toolkit filled with prokaryotes because
Definition
-can be used to cut DNA in specific places, to make many copies of a piece of DNA, and to insert a gene taken from one organism into another
Term
Cutting DNA in specific places requires
Definition
restriction endonucleases from e.coli and other bacteria
Term
Making many copies of a piece of DNA can be done by
Definition
-plasmid from e.coli which the DNA is inserted to. Plasmid is then inserted back into e.coli to grow many copies
-PCR
Term
Inserting a gene taken from organism to another can be achieved by
Definition
Ti plasmid from Agrobacterium tumefaciens which causes crown galls in plants
Term
Polymerase Chain Reaction(PCR)
Definition
any specific segment within a DNA sample can be copied many times using this method (alternative to E.coli plasmids)
Term
Ti plasmid
Definition
most commonly used vector for introducing new genes into plant cells
Term
Prokaryotes are principle agents in
Definition
bioremediation
Term
Bioremediation
Definition
use of organisms to remove pollutants from the environment
Term
Human microbiome project
Definition
determining whether individuals share a core human microbiome
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