Gram Positive

VS

Gram egative

Cell Wall

Cell Parts

&

Functions

Shapes

Reproduction

Growth Requirements:

What do they need?

Growth Requirements:

pH

Growth Requirements:

Temperature

Aerobes

Facultative anaerobes

Anaerobes

Microaerophillics

Normal Flora

obligate pathogens

Opportunistic pathogens

Zoonosis

Fomites

Vector

Reservoir

Carrier

Endemic

Vs

Epidemic

Epidemiology

Host Resistance

Skin

Host Resistance

Mucous Membrane

Host Resistance

Normal Flora

Host Resistance

Inflammation

Host Resistance

Phagocytosis

Immune response

Humoral immunity

Immune response

Cellular Immunity

Host Resistance

Natural Resistance

Tissue invation

Virulence of bacteria

Replication

Toxin Production

Collecting specimens

Swabs

Collecting specimens

Liquids

Collecting specimens

Tissues

Collecting specimens

Feces

Collecting specimens

Anaerobic specimens

Culture Procedures

Direct Examination

Culture Procedures

select and inoculate media

Culture Procedures

Colony morphology

Culture Procedures

Hemolysis

Culture Procedures

Initiate pure culture

if necessary

Culture Procedures

Gram Stain

Culture Procedures

Begin Differential tests to

ID Genus/species

Culture Procedures

Antimicrobial susceptibility testing

Media

Basic nutrient media

Media

Enriched media

Media

Selective Media

Media

Selective / differential Media

Media

Selective / enrichment media

Media

Thioglycollate

Media

Mueller-Hinton

Media

Blood culture bottles

Isolation Streaking

Incubation

Aerobes

Incubation

Anaerobes

Incubation

Microaerophillics

Hemolysis on

Blood Agar

Colony Morphology

GPCOC

Streptococcus

Clinical Significance

Streptococcus

Catalase Neg.

Streptococcus

Hemolysis

Streptococcus

Lancefeild