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Bacteria II
Morphology
34
Microbiology
Graduate
09/30/2009

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Term
Bordetella spp.
Definition
Small, gram - coccobacilli;Media: Bordet- Gengou (potato-blood-glycerol); Obligate aerobe; Colonies are smooth, tiny, glistening (mercury drop or pearl)
Term
Neisseria spp.
Definition
Dipplococci (kidney-shaped) Non-hemolytic; oxidase +; catalase + aerobic G-; non-spore
Term
Enterobacteriacea spp.
Definition
rods Ferments glucose; catalase+; oxidase-; reduce nitrate Facultative anaerobe; G-; Non-spore; LPS (endotoxin)
Term
Escherichia
Definition
Ferments lactose; Motile and non-motile; 3 antigens: O, H, and K
Term
Salmonella
Definition
Lactose-; bile salt resistant; acid and gas production during glucose fermentation; H2S production; Motile
Term
Shigella
Definition
non-motile
Term
Yersinia
Definition
motile
Term
Klebsiella pneumoniae
Definition
Lactase+; Non-motile; Prominent polysaccharide capsule-mucoid appearance
Term
Proteus mirabilis
Definition
Urease +; formation of kidney stones; Motile: swarms
Term
Vibrio spp.
Definition
G- curved rod; Oxidase +; Motile (polar flagella); facultative; Adherence factors
Term
Aeromonas
Definition
Gram- rods; Oxidase+; Motile (polar flagella); facultative
Term
Campylobacter spp.
Definition
G- rod; curved “gull-winged”; Oxidase+; catalase+; Motile (polar flagellum); microaerophilic; grows better at 42 C
Term
Helicobacter spp.
Definition
G- rod; curved or spiral rods (helical, but coccoid in older cultures); Urease+; catalase+; oxidase+; Motile (lophotrichous flagella); microaerophilic
Term
Pseudomonas spp.
Definition
G-bacilli, pairs; Non-fermentative; aerobic respiration of sugars; oxidase+; catalase+; Motile (single polar flagella or polar tuft); Encapsulated; Pili for attachment;
Term
Haemophilus spp.
Definition
Small G- pleomorphic rods (short to long and slender); Facultative; non-motile; fastidious (chocolate agar, X factor: Hemin, V factor: NAD, CO2); Non-spore; encapsulated to unencapsulated
Term
H. influenza
Definition
capsulated
Term
Francisella tularensis
Definition
Small, gram – coccobacilli; Oxidase -; nonfermenter; requires cysteine Non-motile; obligate aerobe; Thin capsule
Term
Brucella melitensis
Definition
Small, gram – coccobacilli;Oxidase +, catalase +, urease +; nonfermenter Non-motile; obligate aerobe; No capsule
Term
Legionella pneumophila
Definition
G – rods (stain faintly, silver stain of tissue), slender rod in nature, coccobacilli in clinical material Require cysteine and iron for growth Obligate aerobes, motile unencapsulated
Term
Bartonella spp.
Definition
Short, G- rods;Fastidious (moist, CO2) ; aerobic
Term
Clostridium spp.
Definition
Large, G + bacilli; Most are catalase-; Most are motile; most are obligate anaerobes; Usually spores
Term
Actinomyces israeli
Definition
Gram+ curved or filamentous rods, not acid fast; Non-motile, anaerobic, slow growth; Nonspore forming
Term
Spirochetes
Definition
Long, slender, G- bacilli; corkscrew or loose coiled shape; Motile; aerobic, anaerobic or facultatively anaerobic; Unique structure for mobility: central protoplasmic cylinder bounded by cell wall, outer sheath/envelope similar to G-, multiple periplasmic flagella: between cell wall and outer sheath, axial orientation (span entire length of cell, anchored at both ends), rotate similar to external flagella, responsible for propelling cell, mechanism unclear; invasion of host tissue by penetration
Term
Trepenoma pallidum
Definition
Tightly coiled; Anaerobic or microaerophilic; needs a cell to grow
Term
Borrelia burgdorferi, B. recurrentis
Definition
Relatively large, thin, coiled (loose, irregular waves); weak staining G- (giemsa stain); Anaerobic or microaerophilic
Term
Leptospira interrogans
Definition
Thin, slender, tightly-coiled, hooked end; Obligate aerobes
Term
Mycoplasma and Ureaplasma
Definition
no cell wall (stain poorly); enclosed in a single, trilaminar plasma membrane (contains sterols obtained externally); smallest of known free-living bacteria (pass through bacteriologic filters)
Term
Rickettsia general
Definition
Gram-, small coccobacilli (Gram stain poorly: Giemsa or Gimenez stain); slime layer; no flagella; aerobic; strict intracellular
Term
Ehrlichia and Anaplasma
Definition
Gram - (Giemsa or Wright stain); obligate intracellular bacteria (grow in cytoplasmic vacuoles=morula)
Term
Chlamydia and Chlamydophila general
Definition
small, round-to-oval organisms, vary in size; inner and outer membrane; LPS but no peptidoglycan (cell wall not well characterized); OMP2: disulfide cross-links; Energy parasites (cannot make ATP)
Term
Chlamydia and Chlamydophila physiology and structure
Definition
Elementary bodies (EB): small (0.3-04 microm), extracellular form, rigid outer membrane (disulfide linked proteins),resistant to harsh conditions, non-replicating form, non-metabolically active form, infectious form (bind to nonciliated epithelial cells or macrophages); Reticulate bodies (RB): larger (0.8-1 microm), intracellular form, fragile membrane (fewer disulfide), metabolically active, replicating form, non-infectious
Term
Chlamydia and Chlamydophila Development cycle
Definition
EB bind to host-->internalization-->inhibition of phagosome-lysosome fusion-->reorganization into RB (breakdown of disulfide)-->growth of RB-->reorganization into EB-->inclusion bodies-->release of EB (lysis-psittaci, extrusion-trachomatis & pneumoniae)
Term
C. trachomatis
Definition
biovars - biological variants (trachoma, lymphogranuloma venereum-LGV); serovars-serological variants (major outer membrane proteins, A-L)
Term
Coxiella burnetti
Definition
Gram-; small cell variants (SCVs): 0.2-0.5 micro, spore-like; large cell variant: 1 micro
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