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Agar test results and meanings
Experimental agar tests and results
34
Microbiology
11/04/2010

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Term
Urea - Fuschia
Definition
Urea - positive for hydrolysis of urea
Term
Urea - Yellow
Definition
Urea - negative for the hydrolysis of urea
Term
LIA - Purple
Definition
LIA - positive for the ability to decarboxylate lysine
Term
LIA - Yellow
Definition
LIA - negative for the ability to decarboxylate lysine
Term
LIA - Burgindy-red on slant
Definition
LIA - positive for lysine deaminase
Term
LIA - No burgund-red on slant
Definition
LIA - negative for lysine deaminase
Term
MIO - agar cloudy (turbid) and stab line not visible
Definition
MIO - Bacterium is motile
Term
MIO - Agar is clear and stab line is visible
Definition
MIO - Bacterium is nonmotile
Term
MIO - (after drops) Xylol layer is red
Definition
MIO - Positive for the presence of indole
Term
MIO - (after drops) No color change in Xylol area
Definition
MIO - Negative for the presence of indole
Term
MIO - Purple
Definition
MIO - positive for the ability to decarboxylate ornithine
Term
MIO - Yellow
Definition
MIO - negative for the ability to decarboxylate ornithine
Term
Nutrient Gelatin - Remains liquid after refridgeration
Definition
Nutrient Gelatin - Positive for the ability to hydrolyze gelatin
Term
Nutrient Gelatin - Solid after refridgeration
Definition
Nutrient Gelatin - negative for the ability to hydrolyze gelatin
Term
MR-VP - (after VP)Bright red-orange sustained
Definition
MR-VP - positive for the presence of acetyl-methyl-carbinol
Term
MR-VP - (after VP) No sustained color change
Definition
MR-VP - negative for the presence of acetyl-methyl-carbinol
Term
MR-VP - (after MR) Red
Definition
MR-VP - positive for high acidity after fermentation of glucose
Term
MR-VP - (after MR) No color change
Definition
MR-VP - negative for high acidity after fermentation of glucose
Term
Citrate - Growth on Agar, Blue agar
Definition
Citrate - Positive for Utilization of citrate as a sole source of carbon, and sufficent gorwth has occurred
Term
Citrate - Growth on agar, Green agar
Definition
Citrate - positive for Utilization of citrate as a sole source of carbon, but sufficent growth has not occurred
Term
Citrate - Growth on agar, Green agar
Definition
Citrate - positive for Utilization of citrate as a sole source of carbon, but sufficent growth has not occurred
Term
Citrate - No growth on slant
Definition
Citrate - Negative for Utilization of citrate as a sole source of carbon
Term
Phenol Red (Sucrose) - Red
Definition
Phenol Red (Sucrose) - negative for the fermentation of sucrose
Term
Phenol Red (Sucrose) - Yellow
Definition
Phenol Red (Sucrose) - positive for the fermantation of sucrose
Term
Phenol Red (lactose) - Red
Definition
Phenol Red (lactose) - negative for the fermentation of lactose
Term
Phenol Red (lactose) - Yellow
Definition
Phenol Red (lactose) - Postive for the fermentation of lactose
Term
Phenol Red (mannitol) - Red
Definition
Phenol Red (mannitol) - negative for the fermentation of mannitol
Term
Phenol Red (mannitol) - Yellow
Definition
Phenol Red (mannitol) - positive for the fermentation of mannitol
Term
TSI - Black butt
Definition
TSI - Positive for the presence of hydrogen sulfide
Term
TSI - Red butt/Red slant
Definition
TSI - negative for fermentation
Term
TSI - Yellow butt/Red slant
Definition
TSI - Positive for the fermentation of glucose, but negative for the fermentation of lactose and sucrose
Term
TSI - Yellow butt/Yellow slant
Definition
TSI - Positive for the fermentation of glucose and positive for the fermentation of lactose and/or sucrose
Term
Phenol Red (all) - Gas bubbles in the Durham tube
Definition
Phenol Red (all) - positive for production of gas during fermentation
Term
TSI - Cracks/gas bubbles in the agar
Definition
TSI - Positive for production of gas during fermentation