Term
| Secretion (Kinds and similarities) |
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Definition
- All cells have constitutive secretion pathway, all the time on (basal rate)
- May require signaling to get to the right place
- Regulated secretion
- Both require synth of content on RER, trafficking/processing to golgi and through
- Regulated has storage compartment reg storage gran |
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Term
Constitutive (mammal)
- Rate
- Effected by outside forces?
- Rate
- Whats in it |
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Definition
| - Occurs constant - independent extracell signals - vesicles are clear in EM - t1/2 ~20min - provides ongoing mem flex to plas mem |
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Term
Regulated Secretion
- Trigger
- Basal rate
- Vesicle appearance
- Speed
- What prevents it from beings constitutively expressed |
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Definition
- Stim by hormonal por depol signals
- Basal rate of discharge is minimal w/o signal
- Vesicles have electron dense but can be clear like Acetylcholine and some catecholamines
- t1/2 ~10 hrs; sits in storage org till needed
- serves as storage organelle for rapid rel of bioactive signals
- Aggregate in TGN key to not being secreted constitutively |
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Term
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Definition
| - Constitutively secreted OR add to plasma membrane! |
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Term
- pH ____ and Ca ____ as you move down pathway going out of cell
- What does this do to proteins? |
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Definition
- Decreases so more acidic because of proton pumps; 6.8-6.5
- Calcium increases
- Acidity + Cal causes clumping of non-constitutive proteins so miss that pathway |
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Term
| Immature secretory vesicle |
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Definition
- Immediate vesicular product of TGN for secretory proteins
- Clatherin used to mature granule, retrieves excess membrane and wayward protein |
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Term
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Definition
- PCs are proteases, synth in the same intracell pathway + packaged along with secretory protein
- PC's can be autocatalyzed or processed by other PC's
- Cleave sec proteins at various sites along pathway
- PC's found in both pathways |
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Term
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Definition
| - Cleaves proteins in TGN; like albumin |
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Term
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Definition
- A giant protein is made that has A and B subunits
- PC2/3 Endoprotease remove C piece then Carboxypeptidase remove the Arg arg
- Maybe PDI to form appropriate bonds |
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Term
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Definition
- is only active in sec granule
- autocatalytic |
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Term
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Definition
- Can be stim by hormone or ion change, or change
- Stim causes rise of intracell Ca++ (NECESSARY)
- SG's move to plasma mem and fuse (Neuron predocked)
- SG content is released by extracell by exocyt
- Inserted SG membranse recycled by endocyt and recycled to TGN |
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Term
| Ca for signaling comes from? |
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Definition
- Plasma channels (usually opened as a result of the signal)
- Intraceullar from ER (SER mostly but RER too) via IP3 receptor signaling |
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Term
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Definition
- Neutralize - charge on vesicles to allow appraoch
- Stim actin binding protein to change cortical actin cytoskl so that vesciles can appraoch
- Relieve a block like synaptogenin |
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Term
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Definition
1) Long skinny proteins that are on membrane and membrane
2) Wrap around each other and physically allow for membrane fusion
3) RABS determine where shit goes to
4) GTP binding protein
5) Need snaps and NSF |
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Term
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Definition
- Snyaptotagmin (and analogs); Ca sensor and complexin (clamp activator)
- Vesicle predocked in neuron, mem cant fuse tho but synaptogenin prevents binding
- Calcium associates with synapt and lets it fuse |
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Term
| Recycling of Vesicles from Membrane(proteins and process) |
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Definition
- Clatherin causes pinching and forming
- Dynamin pinches off and seperates, just go back to endosome and filled up |
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Term
DCV
Filled where?
Go where/how?
What do they contain? |
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Definition
- Dense core vesicles are filled in cell body, trafficked to syn term via axonal and then released
- Peptidergic ntrans and neurohormones |
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Term
SV
Contains (Type/Example)
Constitutive or Regulated?
Refilled when? |
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Definition
- syn vesc
- filled with neurotrans (cholenergic/catecholamine) at the syn term
- SV mem components deliv to synapse via consitutive sec
- refilled after by endocyt |
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Term
| Cell Polarity and Transport |
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Definition
- Sorting can occur in TGN (most pom epith); apical trans protein go trans
- Some sorting occurs in endosomes; stuff goes to basolateral mem, fueses causing a change in signal that then directs it to apical membrane endosomally through trans movement; Igg |
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Term
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Definition
| - guide SNARES to proper destination |
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