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6.1/Molecular Biology
Molecular biology
56
Biology
10th Grade
05/28/2011

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Term
Element
Definition
•Simplest form of a substance—can’t be broken down any further
•Atoms of an element can’t be broken down any further under normal circumstances
•Copper, gold, carbon, etc.
Term
Atom
Definition
•Nucleus with protons+ and neutrons, little elctrons orbit it
•Number of protons in nucleus = atomic number
•Atomic symbol = abbreviation, e.g. Au for gold
•Mass number = sum of protons and neutrons in an atom
•Ground state = electrically equal, same number of protons and electrons •Most stable with enough electrons to complete the outermost orbit level
Term
Isotopes
Definition
•Atoms of the same element with same number of protons but different number of neutrons  different mass number
•As isotopes decay (like 14C  12C) they give off radiation  uses like carbon dating and molecular tagging
Term
Atomic Weight
Definition
•Calculated to account for number and frequency of isotopes
•Weight of all electrons and neutrons of an element in a naturally occurring sample
•Usually a little off from the mass number of most common isotope
Term
Compounds
Definition
•Atom combos held together by a bond
•Help atoms complete outermost orbit levels
•Different physical proterties from their individual elements
Term
Ionic Bond
Definition
•One atom “donates” an electron to another atom
•Atoms get electrically charged  ions
•Ex: Na+Cl
•Held together because opposites attract
Term
Ion
Definition
•Any atom/group of bound atoms that has a positive or negative charge
Term
Covalent bond
Definition
•Two atoms share electrons  molecule
•Sometimes equal sharing (like CH4, methane)  nonpolar covalent bond
•Sometimes unequal sharing (like H2O) because of deifferent electronegativity levels
•Result of unequal sharing is a polar covalent bond with slightly charged parts
Term
Hydrogen Bonds
Definition
•No sharing/donating electrons
•Interactions betweene positive and negative parts of two polar covalent molecules
•Not as strong as covalent or ionic bonds
•Frequent among water molecules
•Stabilize stuff like DNA and several proteins
Term
Life Elements
Definition
•92 natural elements, but only 25 needed for life
•96% of living matter is carbon, oxygen, hydrogen, nitrogen
Term
Cytosol
Definition
• Aqueous part of a cell’s cytoplasm
Term
Polar Molecule
Definition
•Polar covalent bond or ionic bond
Term
Hydrophilic
Definition
•Polar substances that dissolve easily in water
Term
Hydrophobic
Definition
•Nonpolar, don’t dissolve in water; often separate (e.g. oil)
•Simple hydrocarbons and lipids
Term
Carbon
Definition
•4 covalent bonds  versitale, flexible, carbon chains, large molecules
•“C” sometimes omitted in diagrams because hydrocarbons are so common
•Bond with many functional groups
Term
Hydrocarbons
Definition
•Usually hydrophobic
•Simplest carbon chains, just carbon and hydrogen
•e.g. methane/propane
Term
Alcohols
Definition
•Carbon chain bonded to hydroxyl group
Term
Hydroxyl, Carboxyl, Amine, Phosphate
Definition
•Common functional groups that attach to carbon
•Diagrams page 54
Term
Hydroxyl
Definition
• -OH
• Forms alcohol with carbon
Term
Carboxyl
Definition
• -COOH
Term
Amine
Definition
• -NH2
Term
Phosphate
Definition
• PO4
Term
Macromolecules
Definition
• Really big organic molecules—can have thousands of atoms
• Little molecules linked together
Term
Carbohydrates
Definition
• Sugars/linked chains of sugar molecules
• Primary food source for cells
• e.g. starch, cellulose, glucose, fructose (many are –ose)
• Usually a multiple of CH2O formula
Term
Monosaccharides
Definition
• Simplest carbohydrates
• e.g. glucose
• Ring or linear form (ring is more common)
• Covalently bond to each other with dehydration reactions  disaccharides
Term
Glucose
Definition
• C6H12O6
• Cells take their energy from glucose  most important monosaccharide
• Galactose and fructose are isomers
Term
Polymers
Definition
• Big molecules, made up of identical molecules linked together with covalent bonds
Term
Monomers
Definition
• Building blocks for polymers
• Identical
Term
Dehydration Reaction
Definition
• Interaction between hydroxyl groups
• Carbon atom in one sugar and oxygen atom from other sugar bond covalently
• A water molecule is lost
• Commonly used to make monosaccharides into disaccharides
Term
Hydrolysis
Definition
• Add a water molecule to a polymer  it breaks up
• Opposite of a dehydration reaction
Term
Disaccharides
Definition
• Two monosaccharides linked in a dehydration reaction
Term
Isomers
Definition
• Different compounds that have the same chemical formula
• Same atoms, same proportions, different spatial arrangement
Term
Polysaccharide
Definition
• Many monosaccharides linked together
• Store energy: starch/glycogen
• Structure: cellulose, chitin (exoskeletons in insects, spiders, crustaceans)
Term
Starch
Definition
• Polysaccharide made of glucose
• Stores energy in plants when they can’t get it from the sun, humans eat it a lot
• Real name is “amylase”
Term
Cellulose
Definition
• Main part of cell walls in plants
• Polysaccharide
• Very strong  good for structure and can’t be disested by most organisms
Term
Proteins
Definition
• Organic molecules that do most of the work in a cell
• Assemble, disassemble, transport molecules, structure for cells and organisms
• Made of amino acids and several layers of folding
Term
Amino Acids
Definition
• AKA monopeptide
• Amine, side chain (R-group), carboxyl (diagram page 57)
• 20 different types in proteins, identified by R-group
Term
Peptide Bond
Definition
• Made by a dehydration reaction, makes a bond between two amino acids
• Hydroxyl from carboxyl of one amino acid combines with hydrogen from amino side
• Covalent bond between carbon and nitrogen atoms
Term
Polypeptides
Definition
• Chains of amino acids made by peptide bonds
• Always have an N-C-C backbone
Term
Protein Folding
Definition
• Gives proteins their specific shapes
• Primary structure: chain of amino acids
• Secondary structure: alpha helix or beta sheet, hydrogen bonding among amino acids
• Tertiary structure: hydrophobic activity, disulfide bonds, salt bridges
• Quaternary structure: multiple folded polypeptides join together (not present in all proteins)
Term
Denaturation
Definition
• When proteins lose their shape  can’t do their original function
• Wrong temperature, pH, salt concentration, etc.
Term
Nucleic Acid
Definition
• Mainly RNA, DNA
• Instructions for life! Basis of inheritance
• Polymers made of nucleotides
Term
Ribonucleic Acid (RNA)
Definition
• Single stranded nucleic acid
• Ribose sugar
• Uses uracil
• Several different types
o Messenger/mRNA: intermediary between DNA and protein-making process
o Transfer/tRNA: carries amino acids  linked during protein synthesis. Clover!
o Ribosomal/rRNA: helps make proteins, important part of ribosome
o Other small ones are mini-enzymes
o Some viruses use RNA for genetic material
Term
Deoxyribonucleic Acid (DNA)
Definition
• Two strands held together by hydrogen bonding between base pairs
• One 3’ to 5’ strand; one 5’ to 3’ strand
• Deoxyribose sugar (one fewer oxygen than ribose)
• Uses thymine
• Stores the information for the cell—genetic material
• Each mate passes one half to offspring in sexual reproduction
Term
Nucleotide
Definition
• Buiding blocks of nucleic acids
• Structure
o Pentose sugar (5 carbons)
o Phosphate group
o Nucleotide base
• Diagram on page 60
• Adenosine triphosphate (ATP) is a nucleotide with adenine that has three phosphate groups
Term
Pentose Sugar in a Nucleotide
Definition
• Five carbons
• Ribose in RNA
• Deoxyribose (one less oxygen) in DNA
• Covalently bonded to sugar and phosphate group
Term
Phosphate Group in a Nucleotide
Definition
• Phosphate group from one nucleotide binds to sugar of another nucleotide 
• Phosphodiester linkage (dehydration reaction) 
• Sugar-phosphate-sugar-phosphate backbone of nucleic acids
Term
Nucleotide Base in a Nucleotide
Definition
• Stick out from backbone of nucleic acid
• All have nitrogen  nitrogenous bases
• Five types: adenine A, cytosine C, guanine G, thymine T, uracil U
• T only in DNA, U only in RNA
Term
5' and 3' Ends
Definition
• Named for carbon on sugar closest to that end
• 5’ carbon attaches to phosphate group of nucleotide
• 3’ carbon attaches to phosphate group of another nucleotide
• Strands of DNA go in opposing directions
Term
Base Pairs
Definition
• Nucleotide bases stick out from backbone, so they can interact with those of other strands
• Cytosine matches with guanine
• Adenine matches with thymine or uracil
Term
Double Helix
Definition
• Twisted shape formed by two DNA strands
• Figured out by Watson and Crick 1953
• Diagram page 61
Term
Lipids
Definition
• Macromolecules, but not polymers
• Mostly made up of hydrocarbons  mostly hydrophobic
Term
Fats
Definition
• Store energy, lipids
• Long hydrocarbon chains called fatty acids
• Usually triaclylglycerol: three fatty acids with a glycerol
• Stored by many animals as adipose tissue
Term
Steroids
Definition
• Lipids made of hydrocarbons arranged in rings
• e.g. cholesterol (used for structure) and some hormones
Term
Phospholipids
Definition
• Lipids
• Two fatty acid tails attached to a glycerol
• Different from fats because they have a phosphate group with a hydrophilic molecule instead of third fatty acid attached to third carbon of glycerol
• Amphiphathic—glycerol, phosphate group, R-group are hydrophilic head, fatty acids are hydrophobic tails
• Form bilayers
o Used for plasma membrane of all cells
o Few molecules in hydrophilic cytosol can get through hydrophobic part in the middle
Term
Glycerol
Definition
• Three carbons with hydrophilic hydroxyl groups
• Backbone of fatty acids in fats
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