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305 exam3 lec. 2
n/a
23
Biology
Undergraduate 3
11/01/2013

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Term
Sugar breakdown: compare these pathways
Definition
Glycolysis
Entner-Doudoroff
Pentose phosphate
Term
What happens in catabolism?
-- macromolecules= what happens to e-?
-- sugars to -- via --trophy
produce:
Definition
Oxidize macromolecules (pull e- off of them)
Convert into sugars or catabolic pathway intermediates
Oxidize sugars(glucose) to CO2 (Chemotrophy and or heterotrophy and or organotrophy)
Produce
Energy (ATP)
Reducing power (NADH, NADHP, FADH2)
Precursor metabolites (may go to anabolism instead)
Term
Lipid breakdown
Definition
Lipases (hydrolysis) form glycerol and fatty acids -> acetyl-CoA
Term
Protein breakdown
Definition
Proteases (hydrolysis) -> AA
Amino acid decarboxylases (-CO2)and deaminases (remove NH3)-> glycolysis,ect.
Term
Carbohydrate breakdown
Definition
Amylases-> sugars
Term
Glucose formula
Pyruvate formula
Definition
C6H12O6
C3H4O3
Term
nadph is for
Definition
biosynthesis
Term
Aerobic and anaerobic respiration needs
Definition
pyruvate dehydrogenase and TCA cycle)
Term
terminal e- acceptor of respiration
Definition
O2, NO3-, SO4-2
Term
Cytochromes=
Definition
enzymes iron/sulfur clusters (proteins witch move e-)
Iron is useful do to oxidation states/ can add/get rid of e- and still be stable.
Term
Fermentation
-- terminal e- acceptor
no additional-- synthesis
Does not do-
Definition
Terminal organic electron acceptor
Generally no addition ATP synthesis
DO NOT DO oxidative phosphorylation- ATP comes out of glycolic pathway only. You do get NAD+ back.
Term
Entner-Doudoroff rxn formula-
Definition
C6H12O6 + NAD+ + NADP+ + ADP + Pi → 2 C3H4O3 + NADH + NADPH + 2 H+ + ATP
Term
who uses entner-doudoroff
Net?
Definition
Bacteria (v. colera) and archaea
+1 ATP, 1 NADH, 1 NADPH (biosynthesis)
Term
Pentose Phosphate is-
forms-
Definition
Amphibolic
Can form useful anabolic precursor metabolites
Ribose-5-phosphate and erythrose-4-phosphate
Nucleic acid and protein biosynthesis
Term
Pentose Phosphate can also lead to -
net?
Definition
Otherwise leads to glycolysis (catabolic)
Watch out for fermentation
Net= +3 NADPH
Term
example of pentose phosphate
Definition
Lacto Bacilli-
Either go anabolic or catabolic.
Term
Faciltated anaerobes (she named examples!) if they are functiioning anaerobically they will turn off pyruvate dehydrogenase enzyme bc
Definition
if there isnt a lot of O2 around don’t wont to build up a lot of reducing power bc it is hard to get that power back to oxidized state.
Term
glycolysis aka
Definition
Embden-Meyerhof-Parnas” pathway
Term
glycolysis rxn mechanism
Definition
C6H12O6 + 2 NAD+ + 2 ADP + 2 Pi → 2 C3H4O3 + 2 NADH + 2 H+ + 2 ATP
Term
glycolysis
#steps=
net?
Definition
10 steps
Net energy carriers: +2 ATP thru substrate level phosphorylation, +2NADH (microbe determines where NADH goes.)
Term
Kinase/phosphotransferase
Definition
phsophorylation
Term
Dehydrogenase
Definition
Reducing power (NADH)
Term
Isomerase/Mutase
Definition
Same chemical formula, different structures
Isomerization: Rearrangement
Mutation: Functional group shift
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