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24 Gene Expression II
powerpoint lecture
13
Biology
Undergraduate 1
04/08/2011

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Term
transcription initiation complex
Definition
transcription factors & RNA polymerase II bound to a promoter (in eukaryotes)
Term
TATA box
Definition

crucial in forming the initiation complex in eucaryotes 

 

a key component of many promoters used by RNA polymerase II

Term
TBP
Definition

TATA binding protein

 

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Term
What are the two processing steps that occur only on transcripts destined to become mRNA molecules?
Definition
capping and polyadenylation
Term
RNA Capping
Definition
involved modification of the 5' end. The 5' end is the end that is synthesized first during transcription. The RNA is capped with a guanine (G) nucleotide with a methyl group attached. Occurs after about 25 nucleotides have been transcribed
Term
Polyadenylation
Definition
provides newly transcribed RNA with a special structure on their 3' ends. In eukaryotes the 3' end is trimmed, then a second enzyme adds repeated adenine nucleotides (poly-A tail)
Term
RNA processing
Definition

Broad term for the modifications that an RNA undergoes as it reaches its mature form. For a eucaryotic mRNA, processing typically includes capping, splicing, and polyadenylation.

 

both ends of the primary transcript are altered

some interior parts of the molecule and other parts are spliced together

Term
RNA modification
Definition

5' end receives a modified nucleotide cap

3' end gets a poly-A tail

 

  • facilitate export of mRNA from nucleus (through pores)
  • protect mRNA from hydrolytic enzymes
  • help ribosomes attach to the 5' end

Term
introns
Definition
most eukaryotic genes and their RNA transcripts have long noncoding stretches of nucleotides between coding regions called introns
Term
exons
Definition
the parts that aren't introns, they will be expressed or translated into amino acid sequences
Term
RNA splicing
Definition
removes introns and joins exons, creating an mRNA molecule with a continuous coding sequence
Term
spliceosomes 
Definition

carry out RNA splicing

Term
alternative RNA splicing 
Definition
polypeptide encoded depends on which segments are treated as exons during RNA splicing.... greater number of proteins and organism can produce
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