Term
| How quickly do we need to get the agar in the incubator after antibiotic disks have been added? |
|
Definition
| ASAP, but 15min at the longest |
|
|
Term
| When doing a catalase test what do you add to your specimen |
|
Definition
|
|
Term
| positive catalase test will show? |
|
Definition
Bubbles: staph +
No Bubbles: strep - |
|
|
Term
| If you perform a KOH (Potassium Hydroxide) test, what is the additive? |
|
Definition
|
|
Term
| The reagent that is used for the oxidase test is? |
|
Definition
| 1% Tetramethyl-p-phenylenediamine |
|
|
Term
| The MIC zone on your susceptibility testing, the larger it is the lower the concentration of drug needed. True or False? |
|
Definition
|
|
Term
| The reagent needed for a coagulase test is? |
|
Definition
|
|
Term
| The temperature for an incubator for normal culturing? |
|
Definition
| 37 degrees C. 98.6 degrees F |
|
|
Term
| When scoring a CMT, what does the symbol t refer to? |
|
Definition
|
|
Term
| For CMT, at what point do you score an initial CMT? |
|
Definition
|
|
Term
| What is the objective of specimen collection? |
|
Definition
| To get a sample that represents the type of bacteria |
|
|
Term
| We have a draining tract, what are the frequently contaminated with |
|
Definition
|
|
Term
| Your gram negative bacteria will stain what color? |
|
Definition
|
|
Term
| Gram positive bacteria stain what color? |
|
Definition
|
|
Term
| You have a McConkey agar, it is a selective media. It grows bacteria in the present of bile salts and crystal violet. What bacteria is it good for? |
|
Definition
| Enterobacteria. (Lactose fermenting enterbacteriaceae) |
|
|
Term
| What culture media is the best and most commonly used? |
|
Definition
|
|
Term
| Why do we invert the agar plates when we put them in the incubator? |
|
Definition
| To keep the condensation off the agar |
|
|
Term
| What is the primary isolation media? |
|
Definition
| Blood Agar (grow just about anything) |
|
|
Term
| Your enrichment broth is used to grow what? |
|
Definition
|
|
Term
| You pull out a blood agar and have delta hemolysis, this is commonly produced by what bacteria? |
|
Definition
|
|
Term
| Your triple sugar iron agar is used for detection of? |
|
Definition
| glucose fermentation and the production of hydrogen sulfide. |
|
|
Term
| How do you create an oxygen free environment? |
|
Definition
| Putting a candle in a jar and closing it tight. This is will produce carbon dioxide. |
|
|
Term
| You have a McConkey agar, you see a healthy profuse growth, and it is presumptive evidence of what organism? |
|
Definition
|
|
Term
| Your urea tubes turn what color when they are positive? |
|
Definition
|
|
Term
| Before you use a blood culture, you use the thioglycollate broth to grow what? |
|
Definition
|
|
Term
| How many ml’s do you need for a blood culture? |
|
Definition
|
|
Term
| While making the preliminary evaluation of primary cultures, what must you keep in mind? |
|
Definition
|
|
Term
| Nosocomial infections come from? |
|
Definition
|
|
Term
| Motility testing, how do you know if it’s positive? |
|
Definition
| Color o/s stab line. Everything is mixed |
|
|
Term
| What is it called when you are identifying microbes? |
|
Definition
|
|
Term
| What type of organisms do we not isolate? |
|
Definition
|
|
Term
| All viruses are free living. True or false and why? |
|
Definition
| False because they need a host cell to survive. |
|
|
Term
| If an organism is diplicoccus, what does this mean? |
|
Definition
|
|
Term
| How do bacteria reproduce or replicate? |
|
Definition
|
|
Term
| On a blood agar, you see green growth, what type of hemolysis is this? |
|
Definition
|
|
Term
| Complete hemolysis is also called? |
|
Definition
|
|
Term
| No hemolysis is termed what? |
|
Definition
|
|
Term
| If you use crystal violet, iodine and saphrine, what is the name of the staining? |
|
Definition
|
|
Term
| Bacteria prefer what type of pH range? |
|
Definition
|
|
Term
| You must know what a quality incubator must have: |
|
Definition
| heat source, insulation, thermometer, racks, |
|
|
Term
| Brucillis canis needs what type of culture? |
|
Definition
|
|
Term
| gram negative bacilli, aerobic and is oxidase positive, what might it be? |
|
Definition
|
|
Term
| You have a gram positive cocci, that is catalase positive and coagulase positive, what is it? |
|
Definition
|
|
Term
| You have gram positive bacilli, has spores but non hemolytic, what is it? |
|
Definition
|
|
Term
| What does NCCLS stand for? |
|
Definition
| National committee for clinical laboratory standards. |
|
|
Term
| What are the three categories of spores? |
|
Definition
| Central, Subterminal, terminal. |
|
|
Term
| What is the gram stain in order. |
|
Definition
| Purple, Rinse, Yellow, Rinse, Clear (10-15sec), rinse, Red |
|
|
Term
| What is the goal for streaking plates? |
|
Definition
| To grow individual colonies of specimen |
|
|
Term
| What type of bacteria grows best at low temperatures? |
|
Definition
|
|
Term
| What are bacteria that like to grow at high temperatures? |
|
Definition
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