Term
| How quickly do we have to put the agar w/antibiotic disks into the incubator? |
|
Definition
|
|
Term
| When you are doing a catalase test, what do you add to your specimen? |
|
Definition
| 3% hydrogen peroxide (bubble, poistive, staph) (negative, strep) |
|
|
Term
| If you are performing a KOH what reagent do you add? |
|
Definition
|
|
Term
| The reagent used for the oxidase test is what? |
|
Definition
| 1% tetramethol-p-pheneylenediamine |
|
|
Term
| The larger the MIC zone the lower the concentration of drug needed? |
|
Definition
|
|
Term
| The reagent needed for a coagulase test is what? |
|
Definition
|
|
Term
| The temp of an incubator for normal culturing? |
|
Definition
|
|
Term
| When scoting a CMT (california mastitis test) what does the symbol (t) refer to? |
|
Definition
|
|
Term
| At what point do you score an initial CMT? |
|
Definition
|
|
Term
| What is the objective of specimen collection? |
|
Definition
| Obtain the best sample that is representative of the bacteria type |
|
|
Term
| We have a draining tract what are they frequently contaminated with? |
|
Definition
| Bacteria and normal flora |
|
|
Term
| Your gram negative bacteria will stain what color? Gram positive? |
|
Definition
| Negative - pink/red positive - purple |
|
|
Term
| You have a macconkey agar, it is a selective media, it grows bacteria in the presence of bile salts and crystal violet, what is this good for testing for? |
|
Definition
|
|
Term
| What culture media is the best and most commonly used? |
|
Definition
|
|
Term
| Why do we invert the agar plates in the incubator? |
|
Definition
| To keep the condensation off the agar |
|
|
Term
| What is the primary isolation media? |
|
Definition
|
|
Term
| Enrichment broth is used to grow what? |
|
Definition
|
|
Term
| You pull out your blood agar and you have delta hemolysis, this is commonly produced by what kind of bateria? |
|
Definition
|
|
Term
| Tiple sugar iron agar is used for the detection of what and what? |
|
Definition
| glucose fermentation and bacteria that produce hydrogen sulfide |
|
|
Term
| How do you create an oxygen free environment |
|
Definition
| put a candle in a jar with the lid on which creates a carbon dioxide environment |
|
|
Term
| on a macconkey agar you see a large, profuse growth, it is presumptive evidence of what organism? |
|
Definition
|
|
Term
| your urea tubes turn what color when positive |
|
Definition
positive - pink
negative - orange/yellow |
|
|
Term
| before you use blood culture you use thyoglycolade broth to grow what? |
|
Definition
|
|
Term
| how many mL do you need for a blood culture? |
|
Definition
|
|
Term
| While making a preliminary evaluation of primary cultures what must you keep in mind? |
|
Definition
|
|
Term
| Nosocomial infections come from where? |
|
Definition
|
|
Term
| What do i see that proves it's a positive motility test? |
|
Definition
| color throughout the entire tube not just the stab line |
|
|
Term
| what is it called when you are identifying microbes |
|
Definition
|
|
Term
| what type of organisms do we not isolate |
|
Definition
| zoonotic diseases (anthrax, plague, TB, etc) |
|
|
Term
| True or False - All viruses are free living? |
|
Definition
| false - they need a host cell to replicate |
|
|
Term
|
Definition
|
|
Term
| How does bacteria replicate/reproduce? |
|
Definition
|
|
Term
| on your blood agar you see green growth what type of hemolysis is this? |
|
Definition
|
|
Term
| complete hemolysis is also called what? |
|
Definition
|
|
Term
| no hemolysis is called what? |
|
Definition
|
|
Term
| If you use crystal violet, iodine, and saphron what is the name of that staining? |
|
Definition
|
|
Term
| bacteria require what pH range? |
|
Definition
|
|
Term
| know what a quality incubator needs? |
|
Definition
| heat source, insulation, racks, tight door, etc |
|
|
Term
| brucella cateus need what type of culture |
|
Definition
|
|
Term
| you have a gram neg bacilli it is an aerobe and is oxidase positive - what might it be? |
|
Definition
|
|
Term
| you have a gram positive cocci that is catalase positive and coagulase positive what is it? |
|
Definition
|
|
Term
| gram positive baccili it has spores but non hemolytic, what is it? |
|
Definition
|
|
Term
| what does NCCLS stand for? |
|
Definition
| National Committee for Clinical Laboratory Standards |
|
|
Term
| What are the three catagories of spores? |
|
Definition
| central, subterminal, and terminal |
|
|
Term
| Know the gram stain in order |
|
Definition
| crystal violet, rinse, iodine, rinse, decolorizer, rinse, saphron, rinse |
|
|
Term
| What is the goal for streaking plates? |
|
Definition
| Grow individual colonies, spread them out |
|
|
Term
| What type of organisms grow best at low temperatures? |
|
Definition
|
|
Term
| what type of organisms grow best at high temperatures? |
|
Definition
|
|
