Shared Flashcard Set

Details

VT 255: wk 3``
Week 3
48
Veterinary Medicine
Not Applicable
03/26/2015

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Cards

Term
A dog walks in with huge golf ball size lymph nodes. What technique will you use to do cytology tests?
Definition
fine point aspiration
Term
True or False, gram stain in ear cytology must be heat fixed.
Definition
True
Term
For a firm lesion that has few cells, what technique would you use for cytology?
Definition
Scraping
Term
You have a swab sample, how must you take the sample from the swab to the slide?
Definition
Slow, gentle, roll
Term
What can happen if you push the swab too hard on to the slide?
Definition
Damage the cell
Term
A dog has a lesion on its hip. The doctor wants to check for a bacterial infection.
Definition
Imprint
Term
How do you do a Tzanch Preparation
Definition
Require at least 6 slides
- Imprint before the lesion is cleaned
- Clean the lesion with saline and a surgical sponge
Imprint #2 is taken
- De-bride lesion and take imprint #3
- If scab is present, imprint underside of lesion #4
- Imprint exposed tissue #5
Term
A dog has a mass on its liver. What sampling technique would be appropriate for this mass?
Definition
Imprint
Term
What size needle and syringe is needed for a fine needle aspirate of a cat’s lymph node?
Definition
25g needle
3-20g syringe (more than likely 10g Syringe)
Term
True or false, Motility of sperm depends on the amount of swirling activity observed in a drop of semen observed in low magnification?
Definition
False
Term
What procedure uses the stab technique and capillary technique to obtain the sample?
Definition
Nonaspirate procedure.
Term
A fluid sample collected directly from the abdomen is called what?
Definition
Paracentesis/ Abdominalcentesis
Term
What biopsy technique takes minimal time but does require a local anesthetic and maybe one or two sutures?
Definition
Punch Biopsy
Term
A fractious cat comes in presenting with a severe cough and the vet wants to obtain a sample. What is the best method?
Definition
Transtracheal Wash
Term
Testing cerebral spinal fluid that has a low cell count so it needs to be concentrated prior to analysis. How can it be concentrated?
Definition
Either low speed centrifugation or Gravitational sedimentation
Term
A really thick sample that was obtained through fine needle aspirate. What is the best technique to make a slide?
Definition
Starfish smear
Term
A Clinical mastitis sample will usually have what percentage of neutrophils in it?
Definition
up to 95% neutrophils
Term
What type of smear technique makes 3 distinct different areas of cell distribution?
Definition
Combination
Term
What stain works well for staining the cytoplasm of the cell?
Definition
Romanowsky(Diff quick)
Term
A fluid sample with low cellular concentration. What would be the best slide prep technique?
Definition
Line Smear
Term
You need to determine is the cells are neoplastic or inflammatory. What is the best stain to use?
Definition
New-Methylene Blue
Term
You need to send sample to a reference laboratory. How do they need to be submitted?
Definition
Submit 2-3 direct smears and 2-3 Romanowsky Stained smears.
Fluids go into a EDTA/ Red top
Labeled w/ patients history
don't mix unfixed slides w/ formalin
Term
True or false. Dairy farmers never worry about bovine mastitis, clinical or subclinical.
Definition
False
Term
You have a slide that shows cells in various stages of mitotic activity. How would the cell be categorized?
Definition
Malignant
Term
True or False. Prostate problems are not common in cats?
Definition
True
Term
You have a tumor that is highly cellular and comes off in clumps or sheets. What type of tumor could it be?
Definition
Epithelial Cell Tumor
Term
What type of tumor is a melanoma?
Definition
Discrete round cell tumor
Term
Peritoneal and plural fluids are evaluated for TNCC. What is considered to be normal?
Definition
Less than 10,000 nucleated cells
Term
What kind of cell lines the body cavity and become reactive?
Definition
Mesothelial Cell
Term
A fluid sample that includes lymphocytes, plasma cells, WBC’s, neoplastic cells and bacteria would come from where?
Definition
Lymph Nodes
Term
What is the most common method of semen collection
Definition
Massage
Term
Is it important to record the method of collection
Definition
yes
Term
What cytology sample contains evidence of Malassezia and Yeast cells
Definition
Ear Swabs
Term
What stage of the estrus cycle has a high number of RBC's, basil, and parabasil epithelial cells
Definition
Proestrus
Term
You stain a cytology slide for microscopic examination. What step ensures the highest quality preparation?
Definition
Fixing w/ 95% methanol
Term
What does nuclear molding mean
Definition
Deformation of nuclei by other nuclei
Term
What is another name for sarcoma
Definition
Mesenchymal Cell Tumors
Term
What is the word for cell fragmentation?
Definition
Karyorrhexis
Term
What is anisokaryosis
Definition
Any variation in overall size of the nucleus
Term
What is another name for Adenocarsenoma
Definition
Epithelial Cell tumor
Term
What is the word for Slow Cell Death
Definition
Pyknosis
Term
What is Pleomorphism?
Definition
Variability in size and shape of the same cell type
Term
If we are doing microbiologic testing, what technique would require surgical preparation?
Definition
Fine Needle Biopsy
Term
What are the 3 Zones that needs to be shown in a Wedge Biopsy
Definition
Abnormal Tissue
Transition Zone
Normal Zone
Term
What must be recorded in any Centisis Procedure
Definition
Vol. of Fluid
Gross characteristics of Fluid
Term
Explain how to do a compression smear.
Definition
- Expel contents of aspiration onto middle of slide.
- Gently place a second slide at right angle to the first slide
- The spreader slide is quickly and smoothly slid across the prep slide
- Downward pressure should not be placed on the spreader slide. (Excessive cell rupturing)
- Modification (Rotate second slide 45 degrees and lift upward)
Term
True or False. A wedge smear and a blood smear are done the same?
Definition
True
Term
What stain gives excellent nuclear detail and Cytoplasmic detail?
Definition
Papanicolaou Stain
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