Term
| Columbia Blood Agar (CBA) |
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Definition
| used for initial growth and cultivation for further testing |
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Term
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Definition
| used to test fo antibiotic sensitivity |
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Term
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Definition
| used for coagulase testing for Staphylococcus aureus |
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Term
| Advantages of In-House Lab |
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Definition
1. Gram staining
2. Faster turn around time
3. Less chance of contamination of sample
4. Fewer no-growth samples b/c bacteria are cultured quickly
5. Less chance of cultures getting lost |
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Term
| Disadvantages of In-House Lab |
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Definition
1. Takes up tech time
2. Must have someone trained and interested
3. Supplies can become outdated if not used routinely
4. Lots of opportunity for contamination of sample
5. Extra care must be used in selecting lab location and handling samples |
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Term
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Definition
| Used to collect a sample from the patient, must be sterile, must be treated like a surgical instrument and not allowed to touch a contaminated surface |
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Term
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Definition
1. Necropsy samples should be take a.s.a.p. after death
2. When collecting from an organ or tissue, sear the surface of the tissue before cutting open
2. Pieces of tissue under 3mm are most useful |
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Term
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Definition
1. Collect using a sterile needle and syringe
2. Do a sterile prep of the site
3. Do not submit sample in syringe, transfer to a transport tube
4. Label the tube |
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Term
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Definition
1. A.s.a.p. after onset of clinical signs
2. No later than convalescent stage
3. Before treatment
4. After treatment has been stopped for an appropriate amt. of time. |
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Term
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Definition
| Solid or liquid substances used to culture and maintain bacteria, STERILE |
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Term
| Four Steps of Gram Staining |
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Definition
1. Crystal Violet - 1 min
2. Rinse
3. Iodine - 1 min
4. Rinse
5. Decolorizer - 30 sec
6. Rinse
7. Safranin - 1 min
8. Rinse |
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Term
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Definition
| Turns bacterial cells blue |
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Term
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Definition
| Makes crystal violet "stick" to bacteria better |
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Term
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Definition
| alcohol that dissolves lipid layer of gram negative bacteria, dehydrates and closes pores of gram positive bacteria so blue stain is retained, be sure to STOP when run-off is clear, do not decolorize more than 30 sec. |
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Term
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Definition
| Counter-stain, turns gram negative bacteria pink |
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Term
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Definition
| purple color, take up crystal violet stain and do not decolorize |
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Term
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Definition
| Lipid cell wall decolorize, retain safranin stain giving them a pink/red color |
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