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Protei Engineering
Overview
16
Anatomy
3rd Grade
03/03/2013

Additional Anatomy Flashcards

 


 

Cards

Term
Advantage of Recombinant products
Definition
natural product sources may be limited; typically cheaper; avoids immunogenicity; avoids pathogen contamination; highly specific recognition and affinity
Term
disadvantages of recombinant products
Definition
may not have a good half life; more complex production, can be fragile; tend to aggregate to protease in vivo making them ineffective
Term
Recombinant Production
Definition
Was primarily E. coli but has now mainly mammalian cells
Term
IL2 (Proleukin)
Definition
stimulates growth and differentiation of T/NK cells and antibody production by beta cells
Term
IFN-B (Betaseron)
Definition
induces antiviral genes and enhances MHCI
Term
EPO (Procrit)
Definition
Stimulates erythropoiesis (Treat anemia)
Term
GCSF (Neupogen)
Definition
promotes hematopoiesis
Term
Products: Lantus; Neupogen; Neulasta
Definition
Production: E. Coli
Term
Products: Epogen; Enbrel
Definition
Production: Chinese Hamster Ovary (CHO)
Term
Products: Remicade
Definition
Production: Mouse Myeloma SP2/O
Term
Advantages of production in E. Coli
Definition
Well characterized host; rapid growth and ability to produce large amounts of foreign proteins; ease of handling; fermentation well established; no transfer of pathogens
Term
Challenge of production in E. Coli
Definition
Most products are made as inclusion bodies; does not glycosylate so production is limited to proteins that do not require this process; other post-translational processes do not occur the same way as in eukaryotic cells
Term
advantages of production in yeast (S. cerevisaiae)
Definition
can perform some post-translational modifications like glycosylation; short generation time and easily cultured; can be transformed allowing for addition of new genes or deletion through homologous recombination
Term
disadvantages of yeast
Definition
glycosylation between yeast and mammalian cells is quite different and produces different products; humanized yeast are being developed to overcome this problem
Term
keratinocyte growth factor used (with improved stability) to prevent chemo induced mucositis
Definition
Kepivance
Term
Produced in Chinese hamster ovary
Definition
Kepivance; Humalog, Novolog; Aranesp
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