Term
| Advantage of Recombinant products |
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Definition
| natural product sources may be limited; typically cheaper; avoids immunogenicity; avoids pathogen contamination; highly specific recognition and affinity |
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Term
| disadvantages of recombinant products |
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Definition
| may not have a good half life; more complex production, can be fragile; tend to aggregate to protease in vivo making them ineffective |
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Term
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Definition
| Was primarily E. coli but has now mainly mammalian cells |
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Term
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Definition
| stimulates growth and differentiation of T/NK cells and antibody production by beta cells |
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Term
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Definition
| induces antiviral genes and enhances MHCI |
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Term
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Definition
| Stimulates erythropoiesis (Treat anemia) |
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Term
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Definition
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Term
| Products: Lantus; Neupogen; Neulasta |
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Definition
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Term
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Definition
| Production: Chinese Hamster Ovary (CHO) |
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Term
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Definition
| Production: Mouse Myeloma SP2/O |
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Term
| Advantages of production in E. Coli |
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Definition
| Well characterized host; rapid growth and ability to produce large amounts of foreign proteins; ease of handling; fermentation well established; no transfer of pathogens |
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Term
| Challenge of production in E. Coli |
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Definition
| Most products are made as inclusion bodies; does not glycosylate so production is limited to proteins that do not require this process; other post-translational processes do not occur the same way as in eukaryotic cells |
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Term
| advantages of production in yeast (S. cerevisaiae) |
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Definition
| can perform some post-translational modifications like glycosylation; short generation time and easily cultured; can be transformed allowing for addition of new genes or deletion through homologous recombination |
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Term
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Definition
| glycosylation between yeast and mammalian cells is quite different and produces different products; humanized yeast are being developed to overcome this problem |
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Term
| keratinocyte growth factor used (with improved stability) to prevent chemo induced mucositis |
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Definition
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Term
| Produced in Chinese hamster ovary |
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Definition
| Kepivance; Humalog, Novolog; Aranesp |
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