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lab practical 1
labs 1-5
81
Anatomy
Undergraduate 4
02/26/2012

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Term
[image]
Definition
Agranulocyte: lymphocyte
Term
[image]
Definition
monocyte:agranulocyte
Term
[image]
Definition
eosinophil:granulocyte
Term
[image]
Definition
granulocyte:neutrophil
Term
[image]
Definition
basophils:granulocytes
Term
mos
Definition
Term
least common granulocytes
Definition
basophil
Term
most common granulocyte
Definition
neutrophils
Term
second most abundant granulocytes
Definition
eosinophil
Term
basophil function
Definition
contribute to inflammatory response
Term
eosinophil function
Definition
kill parasties with granules
Term
neutrophil function
Definition
phagocytosis,
Term
most abundant Agranulocyte
Definition
lymphocyte
Term
disease that changed shape of RBCs due to a recessive allele
Definition
sickle cell anemia
Term
viral disease caused by epstein-barr virus. characterized by increased production of agranulocytes. HIGHlY contagious
Definition
infectious mononucleosis
Term
normal hematocrit values for men/women
Definition
men-45%
women-40%
Term
high RBC count, usually bc of bone marrow cancer
Definition
polycythemia
Term
cancer that results in over production of leukocytes
Definition
leukemia
Term
universal donor blood
Definition
O
Term
universal blood acceptor
Definition
AB
Term
blood type A can get blood from who
Definition
blood types A and blood type O
Term
blood type AB can get/give blood to who?
Definition
get-A,B,AB,O
give-AB,
Term
what type of antibodies does blood type O have?
Definition
A AND B antiboooooooodies
Term
packed red cell volume formula
Definition
[(packed RBCs)/(total volume)]X100
Term
why is the QRS complex the the largest
Definition
because the ventricle is the largest
Term
what is the only interval that can be changed due to exercise
Definition
t-p interval
Term
cardiac output formula
Definition
stroke volume * heart rate
Term
what is the "lub" sound
Definition
av valves closing during ven. systole
Term
what is the "dub" sound
Definition
SL valves closing during ven' diastole
Term
what are korotkoff sounds
Definition
bp sounds
Term
[image]
Definition
normal cardiac tissue
Term
[image]
Definition
MI tissue
Term
[image]
Definition
atherosclerosis
Term
differences between frog and human heart
Definition
frog-has sinus venosus, humans have AV node
frog-have 1 ventricle, humans have 2
Term
in the frog heart what helps blood stay separate
Definition
the trabeculae
Term
in a frog heart what happens 1st the mechanical and or electrical impulses
Definition
electrical
Term
effecs for the following on a frog heart: 1.temp 2,ca2++ 3.k+ 4.ACH 5.isupurel 6.atropine 7.stretch 8.stimulation
Definition
1.dilation of blood vessels=decreased HR 2.^contractile force 3.down HR, and abnormal heart rhythms. 4.reduce HR 5.^HR 6.^hr 7.^contractile force 8.get another contraction
Term
PO2 determines what
Definition
O2 to Hb binding
Term
AN RBC has more affinity to 02 if what happens
Definition
even more 02 binds to the RBC
Term
in the lamda max graph, what are the two axees?
Definition
y axis-absorbance, x axis is wavelength in nm.
Term
the more P02 there is, will that lead to more or less 02 saturation
Definition
more P02, more saturation
Term
for an exercising cell, is the PO2 higher like 40, or lower like 20?
Definition
lower, like 20 and higer like 40 for a resting cell
Term
what is P-50
Definition
the partial pressure of 02 (so whatever number the PO2 is) when the Hg is 50% saturated.
Term
{image:|}
Definition
Term
[image]
Definition
the P50 is 30
Term
how is the 02-Hg curve affected if there is BPG stripping? which way is teh curve shifted? what happens to 02 binding, and 02 release?
Definition
the curve shift left, which means it takes less pressure for 02 to bind to HG, so 02 binding goes up, and 02 release goes down
Term
what happens when you add DPG
Definition
P50 increases, and now it takes more pressure to bind )2 to Hg
Term
how does cold and hot temp changes affect the 02-Hb curve?
Definition
cold-curve shifts left makeing it easier for Hb to bind 02:P50 drops

hot-P50 increases, which means there needs to be more P02 to bind 02 to the Hg
Term
how does Ph affect the HB02 curve?
Definition
low ph-drops P50..curve shifts LEFT

high Ph-raises P50..curve shifts RIGHT
Term
what 3 factors affect Hb's affinity for 02
Definition
ph, temp, PC02
Term
% saturation formula=
Definition
(A-B)/(A-C)X 100%

A=deoxygenated blood
B-oxygenated blood
C=absorbance after final equilibration
Term
how is the 02-Hg curve affected if there is BPG stripping? which way is teh curve shifted? what happens to 02 binding, and 02 release?
Definition
the curve shift left, which means it takes less pressure for 02 to bind to HG, so 02 binding goes up, and 02 release goes down
Term
what does BPG do in general
Definition
facilitates the release of 02 from Hb
Term

[image]

where are the B and T cells located? Blood vessels?

Definition
in the white pulp, which are those purple circles. blood vessels are in red pulp, the stuff around the purple circles.
Term

[image]

point to Hassall's corpuscles. what organ is this

Definition
thymus
Term
primary lymph organs
Definition
thymus and bone marrow
Term
secondary lymph organs
Definition
peyer's patchers, spleen
Term
what was the gelatin in elisa for
Definition
to prevent non-specific binding
Term
the humoral response uses what mechanism mainly?
Definition
antibodies-B cells, etc
Term
cellmediated response uses what?
Definition
Tcells
Term
polyclonal antibodies
Definition
Polyclonal antibodies are produced by injecting a host animal with a specific antigen that result in producing multiple clonal antibodies that are capable recognize the same antigen
Term
monoclonal antibodies
Definition
Monoclonal antibodies are produced by injecting the host animal with a specific antigen and then culture the same clonal antibodies that released by the plasma cells derived from the same mother B cells
Term
what is BSA and what was it in ELISA?
Definition
the antigen coated on the wells, its Bovine Serum Albumin
Term
what were the positive and negative controls for elisa?
Definition
positive-rabbit and Anti-BSA

negative-no rabbit anti-BSA
Term
for elisa what was the conjugated secondary antibody?
Definition
goat anti rabbit IgG-HRP
Term
in elisa what was HRP
Definition
horse raddish peroxidase
Term
in elisa WTF was the chromogen
Definition
the substrate ABS that reacts with the HRP to produce the positive green result
Term
in elisa, what was the 3rd antigen used?
Definition
pig antigoat IgG HRP...must be a different species
Term
in ELISA what produced the positive green affect?
Definition
HRP+ABTS
Term
what is the order of the items in the ELISA experiment for the green positive color to show?
Definition
antigen/primary antibody/secondary antibody(conjugated with HRP)

add ABTS and it reacts with the HRP complex on the secondary antibooody
Term
explain the hemolytic plaque assay
Definition
it checks for the presence of antiboooodies. a mouse injected with sheep RBCs. the sheep cells are attacked by B cells
Term
for the hemolytic plaque assay what is a positive result
Definition
plaques as a result of cell lysis
Term
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Definition
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Definition
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Definition
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