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(Estrous cycle)

Anestrus/"Quiet phase"

duration=2-3 months

behavior=normal

physiology=normal

cytology=cornified/noncornified squamous

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(Estrous cycle)

Proestrus/"Active phase"

duration:4-20 days

behavior:attracts males but will not be mounted

physiology: firm swollen vulva, "spotting" bloody discharge

cytology:RBC,noncornifed EC, possible bacteria

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(Estrous cycle)

Estrus/"In heat","Breeding phase"

duration:3-21 days

behavior:standing heat

Physiology:vulva swollen(not firm), discharge is less red, lordosis, erect tail

cytology:90% cornified epithelial, small amount of blood cells or bacteria

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(Estrous cycle)

Metestrus/Diestrus: "Regressive phase"

duration: 2-3 months

behavior:normal

physiology:vulva is not swollen (or less so), fading discharge

cytology: PMN's w/i first 3 days

Mast cells

*Note the granules all over the place, tightely packed granules

*if neutrophils present=inflammatory

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Peritoneal fluid from a canine

(How would you collect?, what type of prep?, how would you know if inflammatory or septic?

Collection: Abdominocentesis

Prep: wedge or wedge line

Inflammatory: PMN's present

Septic: bacteria present

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(Liver)

Contains: Hepatocytes+RBCs

Collection:

-micro=impression

-punch/core biopsy=roll prep

-incised wedge=impression

-fine needle aspirate=pull prep

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(Lung)

Contains: pulmonary/alveolar epithelial cells (pneumocytes)

-may also see neutrophils, eosinophils, lymphocytes, plasmacytes, and mast cells

Collection:

-micro=impression

-punch/core biopsy=roll prep

-incised wedge=impression

-fine needle aspirate=pull prep

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(Kidney)

Contains: Renal tubular cells+RBCs

Collection:

-micro=impression

-punch/core biopsy=roll prep

-incised wedge=impression

-fine needle aspirate=pull prep

Contains: Ciliated/non ciliated, cubodial/columnar respiratory epithelium

and mucus strands

Collection:

-micro=impression

-punch/core biopsy=roll prep

-incised wedge=impression

-fine needle aspirate=pull prep

Fat Cells

"chicken wire effect"

Collection:

-micro=impression

-punch/core biopsy=roll prep

-incised wedge=impression

-fine needle aspirate=pull prep