Term
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Definition
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Term
| diff in ribose and deoxyribose |
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Definition
| OH group for ribose on 2' carbpn |
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Term
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Definition
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Term
| transciption/translation molecules |
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Definition
| rna polymerase, mrna, trna |
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Term
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Definition
| splicing (get rid of introns) |
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Term
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Definition
| send in complimentary dna |
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Term
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Definition
| send in complimentary rna |
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Term
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Definition
| ORI (host cell enzymes bind here), DNA, antibiotic resistance gene |
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Term
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Definition
| polymerase chain reaction. uses Taq Polymerase to work, works at the high temperatures required. primer gets it going at the sense and anti-sense 3' end |
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Term
| detection of mutated genes |
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Definition
| gel electrophoresis (smaller or larger cuts based on resistriction site elimination or productin) |
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Term
| therapeutic protein synthesis |
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Definition
| cDNA and plasma vectors in bacteria |
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Term
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Definition
in the glass
in the lifeform
out of the lifeform |
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Term
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Definition
| foreign gene or modified endogenous gene |
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Term
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Definition
viruses (retrovirus -- reverse transcriptase gets it going. adenovirus)
liposomes
polymeric delivery vectors (DNA encapsulated in biodegradable polymers) |
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Term
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Definition
only transduce dividing cells
randomly integrates into host genome
passed on to subsequent cells
less than 7kb gene size |
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Term
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Definition
| transduce non-dividing cells. immunogenic. transient expression in 2-3 weeks |
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Term
| ideal dna delivery vector |
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Definition
| large genes, absence of immunogenicity, targeted delivery, high transduction efficiency, ability to turn on or off gene expression, ability to modulate levels of expression based on things |
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Term
| differences in human and bacterium cell |
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Definition
| size (1 to 20 um) cell wall, organelles, flagella, nucleus |
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Term
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Definition
interphase and mitosis (prophase, metaphase, anaphase, telophase)
prophets metitate about answering the tele |
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Term
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Definition
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Term
| rate of growth of fetus is indicated by... |
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Definition
| increase in cell number, increase in linear length |
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Term
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Definition
| cellular junctions, extracellular matrix |
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Term
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Definition
| the preembryo structure with embryoblast |
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Term
| characteristics of stem cells |
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Definition
| asymmetrical division, differentiation (turns into something else), and potential (lots of options!) |
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Term
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Definition
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Term
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Definition
| progenitor cells, which have a limited number of divides and are pushed in a direction |
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