Term
| Methods for monitoring microbes in wine |
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Definition
culturing and microscopy
metabolite analysis
sensory analysis
molecular analysis |
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Term
| Pros and cons of microscopy |
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Definition
PRO:
- rapid results
-effective for cell counts and viability estimates
-easy to use
-low cost per sample
CON:
-requires 10^5 cells present at least
-distinct morphology required for ID
-sampling errors common due to small volumes |
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Term
| Pros and Cons of Culturing Methods |
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Definition
PRO:
-cheap
-assays viable, living cells
-can be performed in house
-can catch problem before spoilage
CONS:
-slow
-some training req'd
-detection limits
-does not detect viable not culturables |
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Term
|
Definition
4EP, 4EG analysis
-quick, sent to lab, too late if detected must monitor constantly
or Measuring VA |
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Term
| Sensory analysis for ID of spoilage |
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Definition
- need to train staff to recognize taint
-may be too late once noticed
-wine matrix effects cause conflicting results |
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Term
|
Definition
detects ATP using luciferin-luciferase assay, rapid
no information of species or viable cell counts
good for monitoring sanitation |
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