Term
| Why perform Water Quality Testing? |
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Definition
It is important because of the transmission of disease by contact or ingestion of contaminated water...
Oral -> Fecal -> Oral Route |
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Term
| What is Water Bourne Disease? |
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Definition
| Two or more persons with similar illness out of consumption or use of water for drinking |
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Term
| What are the two categories of Water Bourne Disease Microbes? |
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Definition
1. Fecal Bacteria
2. Non-Fecal Bacteria |
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Term
| What are FOUR fecal bacteria microbes? |
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Definition
1. Salmonella (Typhoid)
2. Shigella (Dysentary)
3. E. coli (Pathogen Strains)
4. Vibrio Cholera |
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Term
| What are THREE non fecal bacteria? |
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Definition
1. Pseudomonas - Skin infections
2. Staphylococus
3. Legionella |
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Term
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Definition
1. Gram Negative Rods
2. Faculatative Anaerobe
3. Ferments Lactose - Acid and Gas Production |
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Term
| What organism is an indicator of FECAL CONTAMINATION? |
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Definition
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Term
| What is E. coli an indicator of in Water Quality Testing? |
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Definition
| E. coli is an indicator of potential pathogens |
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Term
| What are the PROS of using E. coli in water quality testing? (FOUR) |
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Definition
1. Always present in fecal contaminated water
2. Survives longer in water
3. Not as harmful to work with
4. Easy to isolate, grow and identify |
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Term
| What are the cons to using E. coli in Water Quality Testing? (TWO) |
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Definition
1. Only detects E. coli
2. Fails to detect pathogens from the environment |
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Term
| What is the media used in the PRESUMPTIVE TEST for total coliforms? |
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Definition
| Lactose Purple Broth Base |
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Term
| What are the results of the Lactose Purple Broth Base? (What is required for a "positive" test?) |
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Definition
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Term
| What are the units of the Water Quality Testing Lab? |
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Definition
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Term
| Which dilution out of the three sets of tubes do you use to determine the most probable number? |
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Definition
| The middle tube - 10 (-1) tube |
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Term
| In the Water Quality Testing Lab, you are doing the PRESUMPTIVE test of the standard analysis of water. What media are you using for this test? What does a positive result look like? |
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Definition
The media is Lactose Purple Broth Base tubes.
A positive result is a yellow color change - starts purple. There is also gas production in the derm tube! |
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Term
| What will the MUG Plate tell us about the water sample? |
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Definition
| If there are fecal coliforms present in the water |
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Term
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Definition
Exopolysaccharide
Capsule like morphology |
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Term
| What does Actinmyces look like? |
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Definition
Brownish/Beige
Gram Positive Rod
Holds the soil together - Antibiotics |
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Term
| What is the MPN? Why do we use it? |
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Definition
Stands for MOST PROBABLY NUMBER.
It is an estimate of the number or organisms in 1 mL of water |
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Term
| Which dilution do you pick from when figuring out the most probable number? |
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Definition
10 -1
Always use the middle dilution! |
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Term
| How do you determine the most probable number? (FOUR STEPS) |
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Definition
1. Determine the number of positive tubes for acid and gas in each set of dilutions.
2. Use the chart with the 3 digit number to determine the MPN
3. Multiple the number by the inverse of the middle dilution (10 -1)
4. You have the MPN total coliforms/mL
(These are the units!) |
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Term
| What is the CONFIRMED test for FECAL COLIFORMS? (What's the media?) |
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Definition
| Brilliant Green Lactose Bile Broth |
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Term
| What does the analine dye in Brilliant Green inhibit? |
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Definition
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Term
| What does the BILE in Brilliant Green Lactose Bile Broth SELECT for? |
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Definition
| Selects for intestinal organisms - Tests for gram negative enterics |
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