Term
|
Definition
| Linear, + ssRNA, nonenveloped- Polio, Hepatovirus and foot & mouth disease |
|
|
Term
|
Definition
| Linear, + ssRNA, enveloped, largest genome- SARS, Canine coronavirus |
|
|
Term
|
Definition
| Linear, + ssRNA, non-segmented, enveloped- Rubella, EEE, WEE |
|
|
Term
|
Definition
| Linear, + ssRNA, enveloped, non-segmented- DENV, JEV, WNV, CSF, BVDV, Hep C |
|
|
Term
|
Definition
| Monopartite, + ssRNA, enveloped, Spherical-filamentous-pleomorphic capsid,- Measles & Mumps |
|
|
Term
|
Definition
| linear, - ssRNA, enveloped, helical capsid- Ebola & marburg |
|
|
Term
|
Definition
| Linear, - ssRNA, Enveloped, helical-bullet capsid- Vesicula stomatis virus & Rabies |
|
|
Term
|
Definition
| - SS RNA, Segmented, enveloped, semi-spherical, filamentous, helical nucleocapsid- Influenza A,B,C,D |
|
|
Term
|
Definition
| Linear,(the only) dsRNA, nonenveloped, replicates in cytoplasm- Rotavirus |
|
|
Term
|
Definition
| Linear ssRNA (2 copies), enveloped- Chicken sarcoma, HIV, HTLV-1 |
|
|
Term
|
Definition
| Circular, dsDNA, nonenveloped- human papilloma virus- horn-like warts on animals |
|
|
Term
|
Definition
| Linear, dsDNA, non-segmented, 2nd cause of colds |
|
|
Term
|
Definition
| Linear, dsDNA, enveloped, tegument proteins in virions- CMV, Alzeheimer's or Bell's palsy |
|
|
Term
|
Definition
| Linear, dsDNA, enveloped, oval or brick shaped capsid, only found in humans, replicates in cytoplasm |
|
|
Term
|
Definition
| Circular, partially dsDNA, partially dsDNA, one strand longer than the other, enveloped, causative agent is picornavirus- encode reverse transcriptase enzymes |
|
|
Term
|
Definition
| linear, ssDNA (the only true), nonenveloped, T=1 symmetr- infects cells that regularly divide, one of the smallest viruses- Parvo for Canine, human and feline, B19 virus |
|
|
Term
| What protein cleavage event is necessary for picornavirus infectivity? |
|
Definition
| VP0 cleaves to yield VP2 & 4 |
|
|
Term
| The first use of a vaccine was |
|
Definition
|
|
Term
| ____________are usually used in diagnostic assays instead of polyclonals |
|
Definition
|
|
Term
| a_____________preparation consists of multiple copies of the same antibody, while __________ antiserum is composed of many different antibodies from the same animal |
|
Definition
|
|
Term
| The 1 step virus growth curve was first successfully performed by: |
|
Definition
|
|
Term
| Which method could be used to determine the concentration of infectious virus in a preparation? |
|
Definition
|
|
Term
| which assay could be used to determine if two different proteins are bound to each other in a cell? |
|
Definition
| immunoprecipitation assay |
|
|
Term
| Which assay could be used to determine whether a protein is present in a cell lysate, using an antibody? |
|
Definition
| immunoblotting (western blot) |
|
|
Term
| Which assay could be used to determine the amount of viral DNA in an infected cell lysate? |
|
Definition
|
|
Term
| Which type of ELISA is used to detect the presence of an antigen? |
|
Definition
| capture method- use known antibody, add serum with unknown antigen, add a secondary antibody and see if they make a sandwich and recognize viral antigen for binding |
|
|
Term
| Which type of ELISA is used to detect the presence of antibodies? |
|
Definition
| Indirect method- use known virus antigen and known antibody and test for the presence of an antibody in the serum which would bind to known. |
|
|
Term
| Which immunostaining method directly recognizes antigen? |
|
Definition
| Primary- direct immunofloresence antibody recognizes antigen |
|
|
Term
| which immunostaining method uses a labeled antibody |
|
Definition
| secondary- indirect immunoflorescence antibody that recognizes primary antibody |
|
|
Term
| What were the discoveries between 1940 and 1960 which are considered to have began the age of modern day virology? |
|
Definition
| Electron microscopy, DNA as hereditary material, dna structure, tissue culture techniques, ultracentrifugation, immunological techniques |
|
|
Term
| WHy is it possible for SV40 virus capsid to have t=6 configuration |
|
Definition
| Each structural unit consists of a single protein (VP1) and VP2 and 3 under the surface. 12 of the pentamers or vertices have 5 neighbors, the other 60 have 6 neighbors, so different subunits have very different types of contact |
|
|
Term
| How would you diagnose a virus that stimulates lots of antibodies in infected cattle but who's viral titer is sometimes high and sometimes low? |
|
Definition
| you could do an indirect ELISA to test for the presence of antibody against virus. Use immunostaining to see if virus is in tissue |
|
|
Term
| How do you calculate the number of infected cells in the 1 step growth curve> |
|
Definition
| Initial infectious center titer minus the initial artificial Lysis titer |
|
|
Term
|
Definition
| the number of new virus particles in a cell |
|
|
Term
| Which family contains members that encode reverse transcriptase enzymes? |
|
Definition
|
|
Term
| What method could you use to "purify" a virus? |
|
Definition
Virus Purification Assay- end point dilutions if virus does not form plaque. Collect plaque with glass pipette
biochemical purification: gel filtration
centrifugation technique: differential centrifugation technique, density gradient technique |
|
|
Term
| How does differential centrifugation differ from density gradient centrifugation? |
|
Definition
| In the different centrifugation technique, you gradually increase the g force with which you are spinning sample where in the last steps the virus pellets at the bottom and you disgard the supernatent. In the density gradient centrifugation, you do not want to pellet the virus. |
|
|
Term
| what is the difference between rate-zonal and isopycnic density gradient centrifugation techniques? |
|
Definition
rate-zonal: the density of the medium< the density of the particles at all points in the tube, used for envelope virus, the virus will form a pellet at the bottom if you do not stop the spin at a certain point. Uses sucrose of glycerol
Isopycnic: at some point in the gradient the density of the medium= the density of the gradient, the particles will migrate to that point and stop and the virus will never pellet even if you continue to spin. used for non-envelope viruses- uses cesium chloride |
|
|
Term
| How to calculate burst size |
|
Definition
final IC assay titer- initial AL
________________________
number of infected cells
plateau - eclipse
_____________
# infected cells
|
|
|
Term
| how to calculate number of progeny |
|
Definition
| total virus at end- unabsorbed virus at the beginning |
|
|
Term
| how to calculate the number of infected cells |
|
Definition
| initial IC titer- initial AL titer |
|
|
