Term
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Definition
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Definition
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Definition
| Stains hematoxylin (blue) |
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Term
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Definition
| Blue dye, binds negatively charged structures (ex: DNA) |
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Term
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Definition
Acidic dye
Binds to basic, positively charged substances (proteins)
Stains red |
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Term
| Phase contrast microscopy |
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Definition
| allow you to observe unstained materials - observing living cells |
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Term
| Differential interference microscopy |
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Definition
| allows you to observe unstained materials, useful for observing living cells |
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Term
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Definition
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Term
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Definition
attached just below condenser lens - controls diameter of cone of light entering the condenser
affects contrast and resolution of image |
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Term
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Definition
| on condensor - balances yellow color of lamp light making it whiter and allowing specimen to appear more natural |
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Term
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Definition
ability to distinguish 2 distinct objects that are close together
as being separate |
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Term
| Size of aperture in light microscopy--smaller aperture = |
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Definition
increased contrast
decreased resolution
decreased light intensity |
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Term
| limit of light resolution in a light microscope |
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Definition
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Term
| limit of magnification of light microscope |
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Definition
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Term
| limit of resolution of electron microscope |
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Definition
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Term
| limits of magnification of electron microscope |
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Definition
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Term
| Transmission electron microscopy (TEM) |
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Definition
electron beam passes through speciman
stained with heavy metal ions |
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Term
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Definition
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Term
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Definition
Osmium, uranium, lead
used in TEM |
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Term
| electron - dense structures |
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Definition
Bind much heavy metal
Block the passage of electrons in TEM
Appear dark on micrographs |
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Term
| Scanning electron microscopy (SEM) |
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Definition
Electron beam does NOT pass through specimen
Surface view
Unsectioned sample
Picture produced as secondary electrons are dislodged from surface --> intensity interpreted by cathode ray --> image produced |
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Term
| Fixture for light microscopy |
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Definition
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Term
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Definition
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Term
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Definition
| Forming cross links between amino groups in proteins therefore preserving native structure |
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Term
| Steps of sample processing - light microscopy and TEM |
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Definition
Fixation (LM: formaldehyde, TEM: glutaraldehyde)
Dehydration (alcohol or organic solvents)
Embedding (LM: paraffin wax, TEM: plastic)
Sectioning via microtome (LM: 10microm, TEM: 40nm)
Mounting - LM: glass slide, TEM: metal grid
Staining |
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Term
| Periodic acid-Schiff staining (PAS) |
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Definition
Histochemical technique - stains polysaccharides magenta
ex: stains mucous secretions |
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Term
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Definition
views fluorescent molecules
1. naturally fluorescent
2. structures labeled with fluorescent probes |
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Term
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Definition
| chemical rxn performed directly on section by adding a "capture reagent" that will interact with an enzyme known to exist within a sample --> color change |
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Term
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Definition
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Term
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Definition
Postganglionic parasympathetic efferent fibers
Supply mucosal glands and smooth muscle of intestinal submucosa |
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Term
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Definition
| parasympathetic ganglion cells down between 2 layers of smooth muscle in intestinal mucosa |
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Term
| Layers of smooth muscle in intestines |
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Definition
Inner circular layer
Outer longitudinal layer |
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Term
| Efferent autonomic neurons for smooth and cardiac muscle |
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Definition
- no specialized junction
- NT released distance away from target-->diffuses through CT and ECM
- usually about 10-20micrm or 1000x greater distance than in synapse |
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Term
| What neurons do not always terminate at a specific synapse |
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Definition
- Smooth and cardiac muscle neruons
- Efferent autonomic |
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Term
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Definition
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Term
| What type of neurons have paravertebral and prevertebral ganglia |
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Definition
| Sympathetic postganglionic neurons |
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Term
| Examples of pre vertebral ganglia |
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Definition
| Caudal mesenteric and celiac |
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Term
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Definition
Sparse reticular and collagen fibers
**fibroblasts |
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Term
| What cells in endoneurium? |
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Definition
Schwan cells and fibroblasts
**WBC and lymphocytes = bad |
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Term
| Vascularized layers in nerves |
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Definition
Epineurium and perineurium
**no vessles in endonerium |
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Term
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Definition
| to leap or dance, occurs at node of rangier |
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Term
| Do afferent neurons have fibrous capsule around ganglia? |
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Definition
| Yes - continuous with epineurium |
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