Term
| Which type of staining (negative, simple, differential) is better for looking at size? |
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Definition
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Term
| How does negative staining work? |
|
Definition
| It stains the background, not the cell |
|
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Term
| In what type of microscopy (bright field/dark field/electron) does negative staining work? |
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Definition
|
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Term
| What dyes are used in negative staining? |
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Definition
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Term
| How does simple staining work? |
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Definition
| one dye is used to stain all cells the same colour |
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Term
| What can you tell from simple staining? |
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Definition
| morphology (shape) and size |
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Term
| What type of dye (cationic/anionic) is used for simple staining? |
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Definition
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Term
| How does differential staining work? |
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Definition
| it causes cells to stain differently based on properties of the cell |
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Term
| What are two examples of differential staining? |
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Definition
| gram stain and acid fast stain |
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Term
| Gram-positive microorganisms have a (thinner/thicker) peptidoglycan layer and a (higher/lower) lipid content than gram-negative microorganisms. |
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Definition
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Term
| Why are gram negative cells easily decolorized when ethanol is added as a decolorizer? |
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Definition
| ethanol dissolves the high lipid cell wall allowing the complex to readily exit the cell |
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Term
| What is the counterstain in a gram stain? |
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Definition
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Term
| What is the primary stain of a gram stain? |
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Definition
|
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Term
| What is the mordant in a gram stain? |
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Definition
|
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Term
| What is the decolorizer in a gram stain? |
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Definition
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Term
| Acid fast microorganisms have a (low/high) wax content in their walls |
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Definition
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Term
| What is required to allow dye in an acid fast stain to penetrate the cell? |
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Definition
|
|
Term
| What is the primary stain in an acid fast stain? |
|
Definition
|
|
Term
| What is the decolorizer in an acid fast stain? |
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Definition
|
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Term
| What colour will acid fast positive organisms be? |
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Definition
|
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Term
| What colour will acid fast negative cells be BEFORE the counterstain? |
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Definition
|
|
Term
| What are two genera of acid fast organisms? |
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Definition
| Mycobacterium and nocardia |
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|
Term
| What are two important examples of Mycobacterium? |
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Definition
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Term
| What do some microorganisms produce that are heat and chemical resistant structures? |
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Definition
| free spores or endospores |
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Term
| What is necessary for the dye to enter the cell in spore staining? |
|
Definition
|
|
Term
| What is the primary stain in spore staining? |
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Definition
|
|
Term
| What is the decolorizer in spore staining? |
|
Definition
|
|
Term
| What is the counterstain in spore staining? |
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Definition
|
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Term
| What are the three genera of spore forming organisms? |
|
Definition
| bacillus, clostridium, and sporsarcinae |
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|
Term
| What colour are the endospores/free spores of Clostridium? |
|
Definition
|
|
Term
| Is Clostridium aerobic or anaerobic? |
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Definition
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Term
| What colour are the endospores/free spores of Bacillus? |
|
Definition
|
|
Term
| Is Bacillus aerobic or anaerobic? |
|
Definition
|
|
Term
| What colour are the vegetative/sporangia of Clostridium? |
|
Definition
|
|
Term
| Is Bacillus gram positive or gram negative? |
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Definition
|
|
Term
| Is Clostridium gram positive or gram negative? |
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Definition
|
|
Term
| What is the bacterial shape of Bacillus? |
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Definition
|
|
Term
| What is the bacterial shape of Clostridium? |
|
Definition
|
|
Term
| What is the bacterial shape of Sporsarcinae? |
|
Definition
|
|
Term
| What colour are the vegatative/sporangia of Bacillus? |
|
Definition
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