Shared Flashcard Set

Details

Translation : Sythesis of Protein
Biochem Lect. 4
20
Biochemistry
Graduate
10/29/2011

Additional Biochemistry Flashcards

 


 

Cards

Term
mRNA determines the sequence of ?
Definition
The amino acids in the protein that is produced.
Term
Polysome?
Definition
many ribosomes attached simultaneously to a single molecule of mRNA
Term
What is the initiation codon?
Definition
AUG, methionine
Term
What provides the energy for chain elongation?
Definition
GTP and Aminoacyl-tRNAs
Term
Proteins are synthesized in what direction?
Definition
N- to C- terminus, following codons of mRNA in a 5' to 3' direction.
Term
Termination codons are?
Definition
UGA, UAG, UAA
Term
Wobble Hypothesis
Definition
variability in the 3bp spot. there are more than 61 tRNAs
Term
Silent Mutation
Definition
a change that specifies the same amino acid
Term
Missense point mutation
Definition
a change that specifies a different amino acid
Term
nonsense point mutation
Definition
change that produces a stop codon
Term
Insertion
Definition
Frameshift! Addition of one or more bases
Term
Deletion
Definition
Frameshift, loss of one or more bases
Term
Frame shifts often cause?
Definition
Premature termination
Term
What are the 4 major processed in translation?
Definition
1. tRNA charging
2. Initiation
3. Elongation
4. Termination
Term
Sickle Cell Anemia is the result of what kind of mutation?
Definition
Missense, Val is made instead of Glu making the hemoglobin hydrophobic
Term
Each Amino acids reacts with _ to form _?
Definition
ATP, aminoacyl-AMP and pyrophosphate
Term
Cleavage of this drives the reaction forming aminoacyl-tRNAs?
Definition
pyrophosphate
Term
Once the AA is attached to the tRNA insertion of the aa on to a polypeptide chain depends on ?
Definition
the codon-anticodon interaction
Term
Initiation in prokaryotes.
Definition
Shine-Delgarno upstream from the start site
Term
What is required to form the initiation complex?
Definition
Inititation factors (IF-1, IF-2, IF-3 in prok, eIF-1, eIF-2... in euk,) ATP and GTP
Supporting users have an ad free experience!