Term
| When considering Trans and Cis acting factors, which is diffusible and function at multiple sites in the genome? |
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Definition
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Term
| When considering Trans and Cis acting factors, which are tied closely to the gene? |
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Definition
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Term
| When considering Trans and Cis acting factors, which involves DNA binding protein, such as transcription factors and some regulatory RNA's? |
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Definition
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Term
| When considering Trans and Cis acting factors, which involves DNA sequence elements such as promoters and binding sites? |
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Definition
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Term
| A linear single stranded polynucleotide that reads 5' --> 3' containing a ribose-phosphate backbone and uses Uracil in place of Thymine, what type of macromolecule is this? |
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Definition
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Term
| Which macromolecule is the only one that has a role in the storage and transmission of information? |
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Definition
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Term
| _________ is the process of DNA template-dependent RNA synthesis catalyzed by the enzyme RNA polymerase? |
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Definition
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Term
| The catalytic activity unique to RNA molecules acts as a ___________? |
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Definition
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Term
| Which type of RNA is an RNA copy of the coding strand of DNA from bacterial genes? |
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Definition
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Term
| Which type of RNA carries the base sequence encoding the amino acid sequence for a protein? |
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Definition
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Term
| Which type of RNA that carries an amino acid into the catalytic site of a ribosome? |
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Definition
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Term
| Which type of RNA base pairs with mRNA to ensure selection of the correct amino acid to be incorporated into a nascent polypeptide chain? |
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Definition
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Term
| Which type of RNA is the structural components of a ribosome? |
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Definition
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Term
| A DNA sequence that can be transcribed into a single RNA molecule and starts at the promoter and ends at a terminator is called what? |
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Definition
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Term
| A transcription start site begins where? |
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Definition
| at the +1 (slide 5 if this is confusing) |
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Term
| The sum of all the RNA molecules produced in a cell under a given set of conditions is called the cellular __________? |
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Definition
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Term
How does transcription differ from replication?
2 ways |
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Definition
| Transcription differs from Replication in that it doesn't require a primer and involves only limited segements of a DNA molecule |
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Term
| What word describes coordinately regulated gene clusters? |
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Definition
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Term
| Open Reading Frames are arranged __ --> __ in a transcription unit? |
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Definition
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Term
| A polycistronic mRNA involves the use of what two things? |
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Definition
| One promoter and terminator |
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Term
A promoter is what kind of acting element/
(cis or trans)? |
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Definition
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Term
| A promoter is found in the genome where _____ ________ binds to initiate transcription. |
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Definition
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Term
| Most transcripts (RNAs) start with a purine (+1) often within the sequence of? _ _ _ |
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Definition
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Term
| Which type of promoter actively initiates transcription continuously for "housekeeping genes" |
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Definition
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Term
| Which enzyme, during transcription, is responsible for transcription initiation and synthesis of the first 10 nucleotides of an RNA chain? |
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Definition
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Term
| Which enzyme, involved in transcription, carries out he transcription elongation? |
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Definition
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Term
| When holoenzymes bind the promoter this is known as what type of complex? |
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Definition
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Term
| Unwinding of DNA 12-15 bp forms a transcription bubble called? |
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Definition
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Term
| Transcription termination results in release of RNA and which enzyme from DNA? |
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Definition
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Term
| Which type of enzyme relieves super-coiling during transcription elongation? |
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Definition
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Term
| Termination of transcription requires which protein? |
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Definition
| Rho (p) (the dependent form) |
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Term
| What is a hexameric protein that binds a nascent RNA chain at a rut site and releases an RNA molecule when it contacts the RNA polymerase? |
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Definition
| Rho (p) (the dependent form) |
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Term
| In Rho-independent termination where does the termination signal reside? |
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Definition
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Term
| During sequence specific DNA binding protein, the recognition helix participates in ______ ______ and __ ___ _____ interactions to make chemical bonds with base pairs. |
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Definition
Hydrogen bonds
Van der Waals |
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Term
| The helix-turn-helix motif is defined by what 2 types of helices? |
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Definition
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Term
| What is the most important step in determining whether a gene will be expressed in a cell? |
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Definition
| Initiation of transcription |
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Term
| Which type of protein binds a positive response element located upstream of a promoter, usually it recruits RNA polymerase to a weak promoter/ |
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Definition
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Term
| What binds an operator at or downstream of a promoter to block RNA synthesis binding to a promoter? |
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Definition
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Term
| Repressor proteins mediate what type of gene regulation? |
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Definition
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Term
| Activator proteins mediate type of gene regulation? |
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Definition
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Term
| All three genes in the operon use what type of metabolism? |
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Definition
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Term
| Name the 3 genes a lac operon has: |
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Definition
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Term
| Name the 3 operator sites of lac operon: |
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Definition
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Term
| Which operator site of lac operons is the highest affinity binding sites, which is the lowest? |
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Definition
O1 is the highest
O2 and O3 are low |
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Term
| Is CRP a transcription acitivator or inhibitor? |
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Definition
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Term
| An SOS response is activated by what? |
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Definition
| Extensive DNA damage (i.e. intense UV) |
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