Term
| What is the cells energy resource? |
|
Definition
|
|
Term
| What kind of work do cells do? |
|
Definition
Movement
Biosynthesis
Active Transport |
|
|
Term
| What is the first law of thermodynamics? |
|
Definition
| Energy can not be created or destroyed |
|
|
Term
| What is the second law of thermodynamics? |
|
Definition
| Events in the universe that will naturally occur, energy will always be released and will increase disorder creating entropy |
|
|
Term
| What is the mechanism that cells use to manage their energy resources? |
|
Definition
| The laws of thermodynamics |
|
|
Term
| Energy can't be created or destroyed but it can be what? |
|
Definition
| Converted to different forms of energy |
|
|
Term
|
Definition
| The flow of energy in a living system |
|
|
Term
| What is energy transduction? |
|
Definition
| The conversion of energy from one form to another |
|
|
Term
|
Definition
| The study of energy changes |
|
|
Term
| How doe the second law of thermodynamics apply to cells? |
|
Definition
| conversion of energy will only occur if entropy is increased |
|
|
Term
| What does the second law say about ATP? |
|
Definition
| When ATP is hydrolyzed some energy is lost as heat not usuable by the cell and some is used to produce movement |
|
|
Term
| What is the energy called that the cell uses to do work? |
|
Definition
|
|
Term
What do the variables stand for
H=G+S |
|
Definition
H Amount of energy
S entropy
G is free energy |
|
|
Term
If the final state has more energy than the initial state then the reaction is what?
ΔG>0 |
|
Definition
|
|
Term
If the final state has less energy than the initial state then the process is what?
ΔG<0 |
|
Definition
|
|
Term
|
Definition
| The Study of reactions outside the cell |
|
|
Term
|
Definition
| The study of reactions inside the cell |
|
|
Term
| What is energy coupling in the cell? |
|
Definition
| The use of exergonic reactions to drive endergonic reactions |
|
|
Term
| What currency do cells use to drive energy coupling? |
|
Definition
|
|
Term
| What are the products of ATP? |
|
Definition
|
|
Term
| What type of reaction is the hydrolysis of ATP? |
|
Definition
|
|
Term
| What is the hydrolysis of ATP going to be used for? |
|
Definition
|
|
Term
| What type of molecule is ATP? |
|
Definition
|
|
Term
| How much energy does an ATP molecule produce? |
|
Definition
|
|
Term
| What reactions can ATP drive and Why? |
|
Definition
Reaction that require less than 7.3 kcal per mol
because some energy will be lost as heat. |
|
|
Term
| What is the mechanism of energy transfer of ATP? |
|
Definition
| The energy is transfered with the phosphate functional group that is removed through hydrolysis |
|
|
Term
|
Definition
| Anything that has had a phosphate functional group added to it |
|
|
Term
| What is dephosphorylation? |
|
Definition
| Anything that has had a phosphate functional group removed |
|
|
Term
| What are enzymes and what are their functions? |
|
Definition
| A special class of proteins that cataylze reactions |
|
|
Term
| What are the characteristics of enzymes? |
|
Definition
1. they are catalytic proteins
2. they do not effect the free energy transfer
3. They speed up reactions of things that can and will occur.
4. They do not change the amount of energy to start an endergonic reaction
5. They exihibit substrate specificity
6. effective in small amounts
7. one enzyme can catalyze a reaction multiple times
8. They are sensitive to environmental conditions
9. have optimal conditions temp and pH.
10. enzymes can be regulated
11. some require assistance from other molecules such as ions |
|
|
Term
|
Definition
|
|
Term
| What is cellular respiration? |
|
Definition
| The process by which cells harvest the energy from organic molecules and converting it to ATP |
|
|
Term
|
Definition
| An organic non protein component for catalysis |
|
|
Term
|
Definition
| Enzymes that remove hydrogen atoms. |
|
|
Term
| What is the function of NAD+? |
|
Definition
| To carry the hydrogen atom broken of by dehydrogenases |
|
|
Term
| What type of molecule is NAD+? |
|
Definition
|
|
Term
| What is the energy of activation? |
|
Definition
| The energy required to destabilize bonds in a reaction |
|
|
Term
| What do enzymes do to the activation energy? |
|
Definition
|
|
Term
| What is the active site of an enzyme? |
|
Definition
| The site of the enzyme where the physical interaction of molecules occurs |
|
|
Term
|
Definition
| Molecules that will interact with the active site of and enzyme |
|
|
Term
|
Definition
| Subtrate bind to the active site then the enzyme goes through a confirmational change that destorts the bonds of the substrates then the products of this all the enzyme to go back to the original confirmation which releases the product. |
|
|
Term
| What are in-vivo characteristics? |
|
Definition
| Things known about structures or processes inside a living cell. |
|
|
Term
| What are in-vitro characteristics? |
|
Definition
| Things known about structures or processes outside the living cell |
|
|
Term
|
Definition
| The mechanism for characterizing catayltic activity of enzymes |
|
|
Term
| What factors are looked at in enzyme kinetics? |
|
Definition
1. substrate specificity
2. at what concentrations of molecules do the enzymes work
3. reaction rate |
|
|
Term
| What do we use enzyme kinetics to study? |
|
Definition
|
|
Term
| What is initial reaction velocity? |
|
Definition
| The change in product over time |
|
|
Term
| What is saturation of an enzyme? |
|
Definition
| The substrate concentration at which every active site is occupied |
|
|
Term
| What is the maximum velocity of an enzyme? |
|
Definition
| The reaction velocity at which you reach saturation |
|
|
Term
|
Definition
| The measure of how well enzyme is binding substrate |
|
|
Term
| What value of km makes it a better fit for the cell? |
|
Definition
|
|
Term
| What types of enzyme inhibition are there? |
|
Definition
| Irreversible and reversible |
|
|
Term
| What are the two mechanisms of reversible inhibition? |
|
Definition
| Competitive inhibition and noncompetitive inhibition |
|
|
Term
| What is competitive inhibition? |
|
Definition
| a molecule that looks like the active site of the enzyme and competes with other substrate to bind with the active site to inhibit the production of that enzyme |
|
|
Term
| What happens when you add more substrate than competitive inhibitor? |
|
Definition
|
|
Term
| What is noncompetitive inhibition? |
|
Definition
| The binding of a molecule to the enzyme at a site that is not the active site which cause the active site to change |
|
|
Term
| What is allosteric regulation? |
|
Definition
| A mechanism for enzyme regulation that is reversible |
|
|
Term
| What is the mechanism for allosteric regulation? |
|
Definition
| An inhibitor molecule that binds to a site that is not the active site called the allosteric site and it changes the confirmation of the enzyme to either inhibit or activate enzymes |
|
|
Term
| What is the first teir of biosynthesis? |
|
Definition
| Breaking down molecules to building block to use them to make molecules of the same kind. |
|
|
Term
| What is the second tier of biosynthesis? |
|
Definition
| They break down of molecules to Acetyl CoA |
|
|
Term
|
Definition
| breaking down a molecule to produce the biosynthetic intermediate to create the same molecule |
|
|
Term
| What is the third tier of biosythesis? |
|
Definition
| The breakdown of molecules to create energy |
|
|
Term
| What does enzyme regulation allow cells to do? |
|
Definition
| To control what molecules are being used for |
|
|
Term
| What is a metabolic pathway? |
|
Definition
| A series of linked reactions |
|
|
Term
| What are transient intermediates in metabolic pathways? |
|
Definition
| Intermediates that are produced and immediately consumed |
|
|
Term
| What are anabolic pathways? |
|
Definition
| Pathways in which we assemble small molecules into macromolecules AKA biosynthesis |
|
|
Term
| What type of reactions are anabolic pathways? |
|
Definition
|
|
Term
| What are catabolic pathways? |
|
Definition
| The breakdown of molecules to their simplest components |
|
|
Term
| What type of reactions are in catobolism? |
|
Definition
|
|
Term
| The control of metabolic pathways are controlled by what? |
|
Definition
Enzyme regulation
usually only one ezyme
and
compartmentation |
|
|
Term
| What makes glucose a good energy source? |
|
Definition
The carbon hydrogen bonds
Taking the hydrogen sharing electrons with carbon and replace them with oxygen
we go from high energy electrons to low energy electrons |
|
|
Term
| What make electrons high energy? |
|
Definition
| When they are being shared equally |
|
|
Term
| When is energy realesed in carbon? |
|
Definition
| When it is moved to a more oxidized state |
|
|
Term
| What are the stages of cellular respiration? |
|
Definition
1. glycolysis
2.TCA cycle, Krebbs cycle, Citric Acid cycle
3. electron transport oxidative phosphorylation |
|
|
Term
| Whats the function of glycolysis? |
|
Definition
| Breaking down glucose into pyruvate |
|
|
Term
What is the function of pyruvate processing?
(TCA cycle, Krebbs cycle, Citric Acid cycle) |
|
Definition
| harvesting high energy electrons with NAD+ |
|
|
Term
| What is the functions of electron transport oxidative phosphorylation? |
|
Definition
|
|
Term
| What are the three mechanisms for ATP synthesis? |
|
Definition
1.Oxidative phosphorylation
2. photophosphorylation
3. subtrate level phosphorylation |
|
|
Term
| What happen in substrate phosphorylation? |
|
Definition
| We start with ADP and an organic molecule that has a phosphate functional group. the phophate functional group will be broken off of the substrate and added to ADP to create ATP |
|
|
Term
| What things go into and what comes out in glycolysis? |
|
Definition
1 6-carbon Glucose to 2-3 carbon pyruvate
ADP to ATP
NAD+ to NADH |
|
|
Term
| What has happened when NADH is created? |
|
Definition
| We have moved high energy electrons off of a carbon skeleton. |
|
|
Term
| What goe into the TCA cycle and what comes out? |
|
Definition
Pyruvate goes in and CO2 comes out
NAD+ to NADH
FADH2
|
|
|
Term
| What goes into electron transport and what comes out? |
|
Definition
O2 goes in H2O comes out
ADP goes in ATP comes out
Electrons carried by NADH and FADH2 and NAD+ comes out and FADH
|
|
|
Term
| Where does glycolysis occur? |
|
Definition
|
|
Term
| Where do the pyruvate and high energy electrons produced by glycolysis get shuttled? |
|
Definition
|
|
Term
| What is the energy harvested by the electron transport chain used to create? |
|
Definition
|
|
Term
| What is the potential energy of the proton gradient used for? |
|
Definition
|
|
Term
|
Definition
| Enzymes catalyze the reactions that remove Hydrogen atoms. |
|
|
Term
|
Definition
| An organic molecule required for dehydrogenase activity |
|
|
Term
| What is the dehydrogase going to move and where is it going to attach it? |
|
Definition
A hydride a hydrogen with its electron and carbons
to NAD+ |
|
|
Term
| What do kinase enzymes do? |
|
Definition
| Transfer phophate functional groups too or from ATP |
|
|
Term
| Why do cells phophorylate glucose? |
|
Definition
|
|
Term
| What enzyme is in glycolysis in regulated by allosteric regulation? |
|
Definition
|
|
Term
| What is the mechanism of making ATP called? |
|
Definition
| substrate level phosphorylation |
|
|
Term
| What is the Net equation of glycolysis? |
|
Definition
| Glucose+2 ADP+ 2 Pi + 2 NAD+→ 2 Pyruvate + 2 ATP + 2 NADH + 2 H+ + 2 H2O |
|
|
Term
|
Definition
| Energy harvest exclusively from glycolysis |
|
|
Term
| What are the extra step added to glycolysis(in Humans) for fermentation and what are they intended to do? |
|
Definition
pyruvate+ lactate dehydrogenase+NADH+H+→ Lactate + NAD+
Regenerate NAD+ |
|
|
Term
| What are the extra steps are added to glycolysis(in yeast) for fermentation? |
|
Definition
1.Pyruvate+ Pyruvate decarboxylase→CO2 + Acetaldehyde
2. Acetaldehyde + NADH + H+ + Alchohol dehydrogenase→ Ethyl alchohol |
|
|
Term
| What happen to pyruvate in the presence of oxygen? |
|
Definition
| It will cross into the mitochondria and be converted into Acetyl CoA |
|
|
Term
| What mechanism of enzyme regulation is glycolysis controlled by? |
|
Definition
|
|
Term
| What is the process of feedback inhibition? |
|
Definition
| Regulation in response to metabolite concentration |
|
|
Term
| What is covalent modification? |
|
Definition
| changing the confirmation of the enzyme through a covalent bond. Ex. phosphorylation and dephosphorylation |
|
|
Term
| What is the key regulation enzyme in glycolysis? |
|
Definition
|
|
Term
|
Definition
| The process of converting pyruvate into glucose |
|
|
Term
| In the presence of oxygen what products of glycolysis are shipped to the mitochondria? |
|
Definition
| Pyruvate and the high energy electrons carried by NADH |
|
|
Term
| What is the endosymbiont theory? |
|
Definition
| The theory that the mitochondria and the chloroplast evolved from an early prokaryote inside a cell. |
|
|
Term
| What evidence supports the endosymbiont theory? |
|
Definition
The two membranes of the mitochondria where one was the plasma membrane of the cell that consumed it
Mitochondrial DNA that is functional
has its own ribosomes
mitochondria devide independently of the host cell and devide by a process similar to binary fission
|
|
|
Term
| What is the pathway of Pyruvate into the mitochondria? |
|
Definition
| It crosses the outer membrane then the inner membrane into the mitochondrial matrix. |
|
|
Term
|
Definition
A. Inner membrane
B. Outer membrane
C. Matrix
D. Cristae |
|
|
Term
| What is pyruvate processing? |
|
Definition
| The conversion of pyruvate to Acetyl CoA |
|
|
Term
| How is Pyruvate Converted to Acetyl CoA? |
|
Definition
| With the enzyme pyruvate dehydrogenase |
|
|
Term
| What is special about FADH? |
|
Definition
| it is the only high energy electron acceptor in the TCA cycle and is directly embedded in the electron transport chain |
|
|
Term
| How many energy harvesting reactions occur in the mitochondria? |
|
Definition
5
1 in pyruvate processing
4 in the TCA cycle |
|
|
Term
| What happens to the electron carried by NADH that were produced in glycolysis? |
|
Definition
| NADH passes its electrons to a carrier with the presence of oxygen and they lose a little energy |
|
|
Term
| How do electron move through the electron transport chain? |
|
Definition
| The electrons pass through the components of the electron transport chain. as they pass through they lose energy in discrete packages. by the time the reach the last component they are now low energy electrons. oxygen then picks up the low energy electrons. the energy that was carried by the high energy electrons is used to create a proton gradient and a voltage as well as a pH greadient. |
|
|
Term
| How does the mitochondria produce ATP? |
|
Definition
| the mitochondria pumps protons into the inner membreane space due to the Proton gradient created by the electrons of the electron transport chain. In the inner membrane ATP synthase is embedded. ATP has two components. The transmembrane domain acts as a proton channel and when it gets the signal it opens up and allow the protons to diffuse back across the membrane. at the catalytic site the protons Physically attatch Which causes the catalytic site to turn which attatches the phophate to the ADP to make ATP |
|
|
Term
| Where does all the ATP made by ATP synthase end up and where does it get transported? |
|
Definition
| In the Mitochondrial matrix then it gets transported out to the cytosol |
|
|
Term
| What is the proton-motive force? |
|
Definition
The gradient created by protons
which is a pH gradient and an electrochemical gradient |
|
|
Term
|
Definition
| The coupling of potential energy of a proton motive force to ATP synthesis |
|
|
Term
| What are the components of the electron transport chain? |
|
Definition
|
|
Term
| What is complex 1 of the electron transport chain? |
|
Definition
| The complex that recieves the high energy electrons from NADH |
|
|
Term
| What is complex 2 of the electron transport chain? |
|
Definition
| The complex that includes succinate dehydrogenase and accepts the electrons from FADH2 |
|
|
Term
| What is complex 3 of the electron transport chain? |
|
Definition
| The complex that recieves all the electrons from complex 1 and 2 |
|
|
Term
| What is complex 4 of the electron transport chain? |
|
Definition
The complex that recieves all the electrons leaving complex 3 also called cytochrome oxidase
is the final stop
the electrons are low energy that are going to be picked up by oxygen to form water |
|
|
Term
| What is significant about the complexed of the electron transport chain? |
|
Definition
| They are multiprotein complexes |
|
|
Term
| How is the precision of movement in the complexes of the electron transport chain maintained? |
|
Definition
| Because electrons are passed to molecules that have a slightly higher electronegativity then eventually they are passed to oxygen which is the most electronegative. |
|
|
Term
|
Definition
| The tendency of molecules to give up electrons |
|
|
Term
| What redox potential do stron reducing agents have? |
|
Definition
| A negative redox potential. |
|
|
Term
| What redox potential do weak reducing agents have? |
|
Definition
| A positive redox potential |
|
|
Term
| What is the reducing agent? |
|
Definition
| The molecule that has given up an electron and has become oxidized |
|
|
Term
| What is the oxidizing agent? |
|
Definition
| The molecule that has accepted an electron and has become reduced |
|
|
Term
What are prostetic groups?
What are conjugated proteins? |
|
Definition
Non protein components.
The protein component |
|
|
Term
| What are the functions of protetic groups in the electron transport chain? |
|
Definition
|
|
Term
| What is the function of Ubiquinone in the electron transport chain? |
|
Definition
| To shuttle electrons from complex 1 to complex 3 and complex 2 to complex 3 |
|
|
Term
| Where are packets of energy lost in the electron transport chain and what is this energy used for? |
|
Definition
Complex 1, complex 3, and complex 4.
to be used by other proteins of the protein complexs to pump protons from the mitochondrial membrane to the inner membrane space |
|
|
Term
|
Definition
| Organisms able to produce their own food supply and produce the worlds food supply |
|
|
Term
|
Definition
| Organisms dependent on the food supply created by the autotrophs |
|
|
Term
|
Definition
| The inner most part of the chloroplast |
|
|
Term
|
Definition
|
|
Term
| Within the stroma is a third set of membrane called what? |
|
Definition
|
|
Term
| What is the interior of thylakoid called? |
|
Definition
|
|
Term
| Where is light energy and ATP harvested and made in the chloroplast? |
|
Definition
| Within the membrane of the thylakoid |
|
|
Term
| What is a stack of thylakoid called? |
|
Definition
|
|
Term
| What are high energy electrons carried by in plant cells? |
|
Definition
|
|
Term
| What is the purpose of light dependent reactions? |
|
Definition
| To harvest light energy to convert it to ATP and to harvest reducing equivalents carried by NADPH |
|
|
Term
| What is the purpose of Light-Independent Reactions? |
|
Definition
| Carbon fixation and synthesis of CHO |
|
|
Term
| What type of reaction is Photosynthesis? |
|
Definition
|
|
Term
| What type of reaction is cellular respiration? |
|
Definition
|
|
Term
| What happens in light dependent reactions? |
|
Definition
| Plant use energy form sunlight to make ATP and to convert low energy electron to high energy electrons NADPH |
|
|
Term
| What is the Calvin cycle? |
|
Definition
| The metabolic pathway of converting CO2 to carbohydrate |
|
|
Term
|
Definition
| The conversion of carbon dioxide to an organic molecule |
|
|
Term
| Where do High energy electron come from in the light dependent reactions? |
|
Definition
|
|
Term
| What is the energy of light dependent on? |
|
Definition
The wavelength of light
a higher wavelength has lower energy |
|
|
Term
| What is the function of photosynthetic pigments? |
|
Definition
| Pigments that have the ability to harvest energy form light but only in a certain range of light wavelength |
|
|
Term
|
Definition
| A package of photosynthetic pigments in a chloroplast |
|
|
Term
| Where is light energy collected by the photosystem sent to? |
|
Definition
|
|
Term
| How many photosystem are in Photosynthesis? |
|
Definition
|
|
Term
| Where does photosynthesis begin? |
|
Definition
|
|
Term
| What happens in photosystem 2? |
|
Definition
1.Light energy harvested is sent to the reaction center.
2. at the reaction center low energy electrons from water(photolysis)are converted to high energy electrons. which is an endergonic process
3.The electrons are physically moved in the reaction center to convert them into high energy electrons using redox reactions.
4. The high energy electrons leave photosystem 2 and are passed off to an electron transport chain. Which functions just like a mitochondrial electron transport chain |
|
|
Term
| What happens in photosytem 1? |
|
Definition
1. as the electrons move throught the electron transport chain they lose energy and create a proton motive force which is used to make ATP via Photophosphorylation which is catalyzed by ATP synthase.
2. the electrons are passed to photosystem 1
3. Photosystem 1 uses light to convert low energy electrons to high energy electrons and passes them off to NADPH |
|
|
Term
|
Definition
| The splitting of water to produce oxygen, electrons, and protons in photosynthesis |
|
|
Term
| What drives the movement of electron from water in photosynthesis? |
|
Definition
| The higher electronegativity of the reaction center of photosystem 2 |
|
|
Term
|
Definition
| An electron shuttler in the chloroplast electron transport chain. |
|
|
Term
| Where will ATP made in the chloroplast go? |
|
Definition
| It will stay and be consumed in the stroma |
|
|
Term
| What is noncyclic photophosphorylation? |
|
Definition
| ATP produced by the flow of electrons from ps2 to ps1 |
|
|
Term
| What is cyclic photophosphorylation? |
|
Definition
| Electrons that are recycled back to ps1 through the electron transport chain to produce a little more ATP without making more NADPH |
|
|
Term
| What is the oxygen we breathe a product of? |
|
Definition
| The light dependent reactions in photosynthesis |
|
|
Term
| What is Ribulosebisphosphate carboxylase and what is its function? |
|
Definition
| Otherwise known as rubisco in the calvin cyclye it attatches carbon dioxide to ribulosebisphophate. |
|
|
Term
| What happens in the first step of the calvin cylcle? |
|
Definition
| RUBISCO attaches CO2 to ribulose bisphospate which immediatly breads down into 2 3-phosphoglycerate |
|
|
Term
| What happens in stage two of the calvin cycle? |
|
Definition
3-phophoglycerate + ATP+NADPH to 1,3-bisphosphoglycerate
1,3 biphosphoglycerate to glyceraldehyde 3-phosphate |
|
|
Term
| What is the third stage of the calvin cycle? |
|
Definition
| The use of ATP to regenerate RUBP |
|
|
Term
| What is C3 photosynthesis? |
|
Definition
| The process of taking CO2 from the atmosphere and converting it into two molecules of phosphoglycerate. |
|
|
Term
What is CAM photosynthesis?
|
|
Definition
| the process of fixing carbon dioxide to malate at night then break it down in the morning |
|
|
Term
| What is C4 photosynthesis? |
|
Definition
| the process of seperating CO2 with rubisco and conversion of it to malate |
|
|