Term
| DNA Synthesis occurs during what phase of Mitosis? |
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Definition
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Term
| DNA Polymerase copies template in what direction? Synthesizes new strand in what direction? |
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Definition
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Term
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Definition
| Unwind the Helix ahead of the replication fork. |
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Term
| Single strand binding proteins do what? |
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Definition
| Hold the DNA in single strand conformation. |
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Term
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Definition
| Prevent extreme supercoiling of the parental helix. Break and rejoin DNA chains. |
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Term
| Prokaryotic DNA Polyermases? |
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Definition
| Pol 1 2 and 3. Pol 1 involved in repair, Pol 3 replicative enzyme. |
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Term
| Eukaryotic DNA polymerases? |
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Definition
alpha, beta, gamma and delta
alpha - replication |
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Term
DNA polymerase requires a ?
And adds what to that? |
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Definition
RNA primer. Catalyzed by a primer.
Adds deoxyribonucleotides to 3'OH of RNA primers/end of growing DNA strand. |
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Term
| DNA gyrase - only in? is? and does what? |
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Definition
| Prokaryotes, Topoisomerase, unwinds 1 coil and reforms with phosophodiester bond. |
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Term
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Definition
| formed by continuously copying the parent strand 3' to 5' toward the replication fork. (Pol 3) |
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Term
| Lagging strand (Okazaki Fragment) |
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Definition
| discontinuous copying 3' to 5' away from the replication fork. |
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Term
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Definition
| removes the RNA primer from the lagging strand. |
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Term
| What are the gaps in the OK fragments filled with? |
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Definition
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Term
| What are the OK fragments joined with? |
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Definition
| Polynucleotide ligase. Catalyses formation of phosphodiester bonds. |
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Term
| DNA polymerase has what kind of proofreading function? |
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Definition
| 3' to 5' exonuclease activity. (Pol 3) |
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Term
| Pol 1 has what function and what proofreading activity? |
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Definition
| Fills gap after RNA primer removal. DNA repair (5' to 3' and 3' to 5') |
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Term
| Pol 2 does what and has what kind of proofreading activity? |
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Definition
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Term
| Norfloxacins inhibit what in, in what kind of cell? |
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Definition
| Inhibit DNA gyrase in bacterial cells. |
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Term
RNA primase =? bp
DNA polymerase alpha = ?bp
DNA polymerase delta = ? bp |
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Definition
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Term
| Proliferating cell nuclear antigen participates in what? |
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Definition
| organizing replication and is used as a proliferating cell marker. |
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Term
| What does Telomerase make to protect against nuclease digestion? |
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Definition
| Hairpin! (folds back and binds to itself in Non- watson and crick manor) |
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Term
| What function does Telomerase have? |
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Definition
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Term
| DNA repair system (4 steps!) |
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Definition
1. recognize deformity in DNA
2. generate gap in DNA
3. fill gap with DNA polymerase
4. Ligate the nick. |
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Term
| Retrovirus contain what as genetic material? |
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Definition
| RNA and use it to synthesis DNA by reverse transcriptase. |
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