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Stains Lab
Staining and bacteria
17
Microbiology
Undergraduate 1
10/16/2013

Additional Microbiology Flashcards

 


 

Cards

Term
Differential Stain
Definition
Uses two stains; Allows us to differentiate between two organisms
Term
Types of differential staining
Definition
Gram Stain, Acid-Fast Stain, Spore Stain
Term
Gram Stain
Definition
Differential staining technique using crystal violet and safranin dies that separate bacteria into two large categories: gram (+) and gram (-).
Term
Negative Stain
Definition
uses a negatively charged stain like nigrosin, which is not taken up by the cytoplasm of negatively charged bacteria. Background is stained, not the bacteria.
Term
Bacteria commonly viewed with negative stain
Definition
Streptococcus pneumonia, Klebsiella pneumonia, Clostridium perfringens: bacteria with well-developed capsules
Term
Spore Stain
Definition
The primary dye, malachite green, dies the spore wall, but is washed off the cell wall with a decolorizer. Cell walls are counterstained with safranin. Spores left green. Used with Bacillus and Clostridium.
Term
Acid-Fast Stain
Definition
Stains the cells of the genera Mycobacterium and Nocardia. Their cell walls have a waxy lipid substance and do not take water based stains well. The cell is determined to be acid-fast or not if it holds the primary stain.
Term
Gram category and morphology of Bacillus
Definition
Gram positive bacilli
Term
Gram category and morphology of Clostridium
Definition
Gram positive bacilli
Term
Gram category and morphology of Staphylococcus
Definition
Gram positive cocci
Term
Gram category and morphology of Streptococcus
Definition
Gram positive cocci
Term
Gram category and morphology of Escherichia
Definition
Gram negative bacilli
Term
Gram category and morphology of Klebsiella
Definition
Gram negative bacilli
Term
Gram category and morphology of Mycobacterium
Definition
Gram positive bacilli
Term
Gram category and morphology of Neisseria
Definition
Gram negative cocci
Term
Gram category and morphology of Pseudomonas
Definition
Gram negative bacilli
Term
Gram category and morphology of Salmonella
Definition
Gram negative bacilli
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