Term
| Types of instruments are used for transferring pure colonies to different types of agar (2) |
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Definition
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Term
| When is each type of instrument used, or for what type of agar container is each instrument used for? |
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Definition
Needle: For Deeps
Loops: For slants, plates, and broth |
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Term
| What are the three types of structure of bacteria? |
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Definition
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Term
| Describe what a staphylocci would look like? |
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Definition
| Cocci stuck together in a ball-like shape |
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Term
| Describe what a diplococcus would look like? |
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Definition
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Term
| What is the primary stain in a Gram Stain procedure? |
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Definition
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Term
| What is the counterstain in a Gram stain procedure? |
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Definition
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Term
| Why do we look for single colonies? |
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Definition
| In order to decrease cell density from the sample and obtain individual cells so that one can identify the cell. |
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Term
| How do you streak an agar plate? |
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Definition
| Into four quadrants, using the loop to spread the sample. |
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Term
| What is a simple stain? Example also. |
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Definition
It uses one dye.
Crystal violent: it is used to identify bacteria. |
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Term
| What is a differential stain? Example also. |
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Definition
It uses two dyes one is a primary stain, the other is a countertain.
For example: Gram stain. |
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Term
| What does an endospore stain test for? What types of bacteria should it be used for? |
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Definition
| It tests for the presence of spores. It should be performed on gram positive rods. |
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Term
| What does an acid fast stain test for? |
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Definition
| Acidic lipids in the cell wall (mycolipids) |
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Term
| What is the total magnification used on specimen when the oculars are 10x and the lense being used is 40x? |
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Definition
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Term
What is the power of each objective lense?
Red?
Yellow?
Blue?
White? |
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Definition
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Term
| What is the name of the smaller knob on the sides of the microscope? |
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Definition
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Term
| What lense is used for oil immersion? |
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Definition
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Term
| What is a bunsen burner used for? |
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Definition
| To heat object or sterilize objects. |
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Term
| Explain the process which to transfer a pure culture of a bacteria to an agar plate. Hint: Remember sterilization. |
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Definition
| Set up the bunsen burner. Ignite using the flint/matches. Using a loop, place it into the tip of the blue part of the flame and slowly pull the loop through it. Allow it to cool for only a moment. Touch the loop to the center of the agar plate. Then, using your pinky on the hand you are holding the loop in, untwist the cap of the container with the bacteria and place the loop inside. After, recap the test tube. Spread the sample across one part of the agar. Then, sterilize. Repeat until you have finished the 4 quadrant pattern. Sterilize the loop again and cover your agar plate. |
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