Term
| What are the steps of tissue preparation? |
|
Definition
Fixation
Processing
Embedding
Sectioning
Staining |
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Term
| What does fixation do to a tissue sampe? |
|
Definition
Stops metabolism
Stops enzymatic reactions
Kills pathogens
Hardens tissue to a degree |
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|
Term
| What does fixation do to a tissue sampe? |
|
Definition
Stops metabolism
Stops enzymatic reactions
Kills pathogens
Hardens tissue to a degree |
|
|
Term
| What chemical is often used for fixation? |
|
Definition
Formulin 10%buffered
Glutaraldehyde
Osmium tetroxide for EM |
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|
Term
| What substances are often used for embedding? |
|
Definition
Parafin
Plastic, used less often |
|
|
Term
| What part of the slide preparation process is glutaraldehyde used in? |
|
Definition
|
|
Term
| What is osmium tetroxide used for? |
|
Definition
|
|
Term
| What is the thickness of slides for light microscopy sectioning?EM? |
|
Definition
5-15 micromemeters
1 micrometer |
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Term
| What are some typical staines and what do they highlight? |
|
Definition
Hematoxylin stains nuclear DNA and cytoplasmic RNA most heavily.
Eosin stains eosinophilic items pink and is used as a counter stain with hematoxylin |
|
|
Term
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Definition
| PAS or periodic acidshift stains carbohydrates and can be effective in staining nodular glomerular sclerosis in the kidney due to the increased concentration of carb that accumulate. |
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|
Term
| What is GMS stain used for? What does it stand for? |
|
Definition
Staining organisms, particularlarly for screening for fungi.
Grocotts methenamine silver
Gringots underfround in the dark whre fungus will grow |
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Term
| What stain is used to detect mycobacterium? |
|
Definition
| Ziehl neelson acid fast stain. |
|
|
Term
| What stain is best for detecting spirochetes? |
|
Definition
Warthin Starry silver stain
Sex under the stars will give you syphilis |
|
|
Term
| What is an effective stain for identifying leukocytes in a blood smear? |
|
Definition
|
|
Term
| What is phase contrast microscopy used to view? |
|
Definition
|
|
Term
| What are six different forms of microscopy? |
|
Definition
Light
Phase contrast
Dark field
Fluorescence
Polarizing
Em, transmission and scanning |
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