Term
| What are the goals of a good smear? |
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Definition
1. cause cells to adhere to the slide so they're not washed away during subsequent staining/washing
2. shrinkage of cells does not occur
3. thin smears |
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Term
| How does one prepare a smear if the bacteria are growing in a liquid medium? |
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Definition
| Place 2 or more loopfuls of the liquid medium directly on the slide |
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Term
| How does one prepare a smear if the bacteria grow in solid medium? |
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Definition
| Place 1 or 2 loopfuls of water on the slide and then use an inoculating loop to disperse the organism in the water. |
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Term
| How much material one needs to make a good smear from solid medium? |
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Definition
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Term
| What is the purpose of heat fixation? |
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Definition
| It kills the cells so they adhere to the glass and don't get washed away. |
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Term
| What causes a stain to adhere to bacterial cells? |
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Definition
| The positive charge of the edge will adhere to the negative charged cell surface structures. |
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Term
| Why kind of dyes won't adhere? |
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Definition
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Term
| In which staining technique a basic dye is used? |
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Definition
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Term
| In which staining technique a stain that does not penetrate cells is used to color the background? |
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Definition
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Term
| Which staining technique is useful for visualizing spirochaetes? |
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Definition
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Term
| Which type of staining technique is useful for visualizing the glycocalyx of certain bacterial species? |
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Definition
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Term
| In which staining technique are cells mixed with a stain before they are smered on the slide? |
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Definition
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Term
| In which staining technique is heat fixation of the slide not recommended? |
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Definition
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Term
| In which staining technique is water used to remove excess stain from the slide? |
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Definition
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Term
| Which substances can one use for the simple stain procedure? |
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Definition
Crystal violet
methylene blue |
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Term
| What can one use for the negative stain procedure? |
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Definition
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Term
| Before heat fixation, a wet smear of bacterial cells on a slide must first be ... |
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Definition
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Term
| Bacterial capsules can consist of ... |
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Definition
polysaccharides
polypeptides with unique amino acids |
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