Term
| Phospholipid called phophatidyl inositol4,5 bisphosphate (PiP2) reacts with phosholipace C to make what? |
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Definition
| Inositol triphosphate 1,4,5 (IP3) which is analogous to camp and diachlglycerol. DAG. |
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Term
| What two Kinases become activated? |
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Definition
| Protein Kinase C and Ca2+ calmodulin activated kinase Cam kinase |
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Term
| Where is the receptor for IP3? |
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Definition
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Term
| Where is the protein for DAG? |
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Definition
| Protein Kinase C is found in the membrane |
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Term
| How does the calcium work? |
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Definition
| It binds to calmodulin actives kinase |
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Term
| What is the major regulator of protein kinase C? |
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Definition
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Term
| Protein Kinase C is folded in a way that it blocks its own active site making it inactive? How does it activate? |
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Definition
| When you generate DAG in the membrane the seed domains change the structure of the protein and opens the active site. |
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Term
| How does calmodulin work? |
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Definition
| It needs to bind 4 calcium which binds to CAM kinase peptide. LOts of enzymes are activated by activated activated calmodulin |
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Term
| Know that a hormone can work through more than one receptor. Why? |
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Definition
| Can tap into multiple regulatory pathways. |
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Term
| Tyrosine Kinases active on which molecule? |
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Definition
| Tyrosine. The others do serine. |
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Term
| What is an example of a tyrosine kinase receptor? |
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Definition
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Term
| What do tyrosine receptors usually regulate? |
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Definition
| Growth nd platelets. TKR are usually associated with insulin and cancer |
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Term
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Definition
| dimzerizaton, cross phosphorylation which generates docking sites for signaling molecules in the cytoplasm. |
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Term
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Definition
| Is a protein that can bind to an activated tyrosine but binds in a different location. |
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Term
| Sh2 Domains all recognize activated tyrosine proteins. How do they know which activated protein to bind to? |
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Definition
| The SH2 domains bind only to phosphorylates tyrosine surrounded by specific amino acids. |
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Term
| Proteins will have a domain to tell. It to acticate and? |
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Definition
| To tell it where to go in the cell. Can be regulated by location. |
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Term
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Definition
| dimerize, cross phosphorylation, causes grb2 to recognize to the receptor and binds allowing Sos to bind thru Sh3 domain. soS has a GDP which is activated to GTP. |
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Term
| Sh3 binds to proteins with? |
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Definition
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Term
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Definition
| Activates RAf kinase which activated MAPkinase. Regulates cell growth...cancer |
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Term
| What are oncogenes and what is an example |
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Definition
| A mutated gene that controls growth. Ras gene |
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Term
| The proteins are constitutively activated. What is that |
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Definition
| Normal cells ar forced to make them and tae on properties of tumor cell |
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Term
| What is the biochemical defect? |
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Definition
| Single amino acids prevent protein from hydrolyzing GTP to GDP. |
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Term
| What disease are consitutively activated proteins similar to? |
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Definition
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Term
| One amino acid ras change is responsible for which cancers |
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Definition
| Pancreas, lung, colon, liver, skin. All but brain, breast, prostate, ovary. |
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Term
| RAS must be turned off by what molecule? |
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Definition
| NF1. If you can't turn off RAS you get schwannoma. Tumor suppressor gene. Loss of function causes cancer. |
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Term
| Similarities between FTP and TKR |
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Definition
| All involve GTp and activation of cytoplasmic kinase |
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Term
| What are the differences between TKR and and G protein? |
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Definition
| No second messenger for TKR. TRK functions to target molecules to the cell surface where they can function. |
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Term
| How is there overlap between tyrosine Kinase and PLC |
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Definition
| Some TKR can activate PLC |
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Term
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Definition
| Know the difference between phospholipase C. what similar and what different between them to allow them to bind to different receptors. SLide 25 |
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