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Science Foundations II
nucleotides and nucleosides
20
Health Care
Professional
11/29/2008

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Term
nucleoside components
Definition
nitrogenous base, pentose (no phosphate)
Term
nucleotide components
Definition
nitrogenous base, pentose, phosephate group
Term
beta-glycosidic bond
Definition
links the nitrogenous base to the pentose sugar (beta = above plane)
Term
pentose 1'C
Definition
links to nitrogenous base
Term
pentose 2'C
Definition
group attached signals whether DNA (H) or RNA (OH)
Term
pentose 3' and 5'C
Definition
link together to form backbone
Term
purine
Definition
adenine and guanine, 2 ring base structure
Term
pyrimidine
Definition
cytosine, thymine (DNA), and uracil (RNA), 1 6 member ring base structure
Term
nucleotides used as:
Definition
building blocks of nucleic acids, chemical energy (ATP), secondary messengers (cAMP), cofactors
Term
Where does ATP bond break to release the most energy?
Definition
alpha-beta anhydride linkage = 46 kJ/mol
Term
phosphodiester bond
Definition
links successive nucleotide units
Term
5' end terminates in:
Definition
a phosphate group
Term
3' end terminates in:
Definition
a hydroxyl group
Term
Chargaff's rules
Definition
base composition of DNA varies from species to species, different tissues of same organism have same base composition, base composition does not change (with age, health, etc.), in all DNA # of A=T and G=C
Term
DNA characteristics
Definition
hydrophilic backbone, 10 base pairs/turn, antiparallel and complimentary strands, hydrophobic bases, beta-DNA (right handed double helix) prominent form
Term
bonding between base pairs
Definition
provides for complimentary strands, A=T (2H bonds), G=C (3H bonds)
Term
denaturation
Definition
disruption of H bonds between bases, strand backbone remains intact
Term
annealing
Definition
spontaneous association of individual, complementary DNA chains
Term
differences between RNA and DNA
Definition
in RNA, T replaced by U, single-stranded, ribose (2'C has OH group), not as stable
Term
RNA functions
Definition
can be in viral genome, mRNA - transmits info encoded by DNA to protein sequence, tRNA - adapter in protein synthesis, rRNA - part of ribosomal structure, ribozymes - catalytic activity
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