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Robinson
Photosynthesis
29
Plant Sciences
Undergraduate 2
11/02/2016

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Cards

Term
Describe the composition of a chloroplast
Definition
Contains thylakoids, which stack into grana, all surrounded by stroma
Term
Give the photosynthesis formula and name what is oxidised and what is reduced.
Definition

6 CO2 + 6 H2O ? C6H12O6 + 6 O2


Water is oxidised

Carbon dioxide is reduced

Term
Photosynthesis is a combination of what two processes?
Definition
Light reactions and the calvin cycle (dark reactions)
Term
Light reactions convert solar energy into ____
Definition
Chemical enery, ATP and NADPH
Term
What are the different types of chlorophyll and what are their functions?
Definition

Chlorophyll a is the main photosynthetic pigment

Chlorophyll b is an accessory pigment, it absorbs different wavelengths of light because of its porphyrin ring and passes the energy to chlorophyll a

Term
What is the role of chlorophyll?
Definition
To absorb light and go from a ground state to an unstable excited state and release electrons
Term
What is a photosystem?
Definition
A reaction center surrounded by a number of light-harvesting complexes
Term
Describe light harvesting complexes
Definition

Consist of pigment molecules bound to proteins

Funnel the energy of photons of light to the reaction center

Term
What happens when a reaction center chlorophyll molecule absorbs energy?
Definition
One of its electrons gets bumped up to a primary acceptor
Term
Where are photosystems I and II found?
Definition
In the thylakoid membrane
Term
What happens when the primary acceptor receives electrons from the reaction center chloroplasts?
Definition
Depends on the photosystem, in photosystem I it triggers the production of NADPH from NADP+ reductase after the electron transport chain , in Photosystem II it triggers the production of ATP from the cytochrome complex during an electron transport chain, but also initiates Photosystem I through the reaction center (linked)
Term
What is the main purpose of the dark cycle (the calvin cycle) and where does it occur?
Definition

Uses ATP and NADPH to convert CO2 to sugar

Occurs in the stroma, outside the thylakoids

Term
Why is the Calvin cycle called the dark cycle?
Definition
Because it doesn't require the input of light
Term
What does the calvin cycle return to the light cycle?
Definition
ADP, inorganic phosphate and NADP+
Term
In photosynthesis, water is split and oxygen released into the atmosphere.  Does this occur in the light or dark reactions?
Definition
Light
Term

Name the three phases of the Calvin cycle in C3 plants and name the key molecules involved in each

 

1. Carbon fixation - Rubisco then ATP

2. Reduction - NADPH (sugar output)

3. Regeneration of Rubisco - ATP

Definition
Term
In photosynthesis, water and carbon dioxide enters; oxygen leaves.  True or false?
Definition

False, light also enters

 

Term
What happens in the calvin cycle during hot weather in c3 plants?
Definition
Stomata close up, causes oxygen build up, rubisco starts incorporating oxygen instead of carbon dioxide, photosynthetic rate is reduced
Term
How do C4 plants minimize the cost of photorespiration?
Definition
By spatially confining the calvin cycle to very internal cells: carbon dioxide incorporated into four carbon organic acids (c4) in mesophyll cells by PEP carboxylase (NOT Rubisco).  PEP carboxylase is not too sensitive to 02/CO2 ratios and can incorporate CO2 at very low concentrations.  C4 is then exported to bundle sheath cells, where they release C)2 used in the calvin cycle
Term
Are C4 plants better than C3 plants?  Describe another type of plant that could be more efficient
Definition

Only at optimal (high) temperatures, at low temperatures C3 is more efficient

 

CAM plants: Temporal separation vs spatial separation - open stomata at night, incorporating CO2 into organic acids, close stomata during the day and CO2 is released from organic acids and used in calvin cycle

Term
What is the connection between thylakoid stacks called?
Definition
Lamellae
Term
What is the inner space of a thylakoid called?
Definition
Lumen
Term
Which are faster, light reactions of dark reactions?
Definition
Light reactions are very fast, comparitively dark reactions are very slow (3.5 events per second)
Term
What generates ATP and NADPH during photosystems I and II?
Definition
The electron transport chain, proton gradient drivest ATPase and NADP+ reductase
Term
Where is starch and sucrose synthesised and stored?
Definition
Starch is synthesised and stored in chloroplasts, sucrose is synthesized in the cytosol and transported throughout plants
Term
What 'fixes' CO2 into a 5C sugar during the Calvin cycle?
Definition
Rubisco
Term
Name the inputs and outputs of 1 turn of the Calvin cycle
Definition

Input: 3 ATP, 2 NADPH

Output: 1 molecule of CO2 fixed

 

 

Term

3 CO2 + 6 NADPH + 5 H2O + 9 ATP > glyceraldehyde-3-phosphate (G3P) + 2 H+ + 6 NAP+ + 9 ADP + 8 Pi (Pi = inorganic phosphate)

 

What is this formula?

Definition
CO2 fixation in a C3 plant
Term
How does rubisco end up with two 3C sugars (PGA)?  And how can PGA be used to generate 6C sugars like glucose and fructose?
Definition

Fixes CO2 into 5C sugar, resulting 6C sugar is untable and breaks down into 2 3C sugars.

 

PGA is converted into G3P by NADPH and ATP which can be used to generate 6C sugars

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