Term
| Which fungi has aseptate hyphae? |
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Definition
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Term
| What are the Zygomycota genuses? |
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Definition
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Term
| What are the dimorphic fungi? |
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Definition
| Blastomyces, Histoplasma, Coccidioides, Paracoccidioides |
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Term
| What makes a fungi dimorphic? |
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Definition
At room temp it is a mold
At 37C it is a yeast |
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Term
| What kind of mycoses do the dimorphic fungi cause? |
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Definition
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Term
| What kind of hyphae do the dimorphic fungi have? |
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Definition
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Term
| What does saprophytic mean? |
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Definition
| Gets nutrition from dead things |
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Term
| Which yeast can show hyphae or pseudohyphae in direct detection? |
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Definition
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Term
| What bleaching agent is used for fungi detection? |
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Definition
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Term
| What are the roles of the components of lactophenol cotton blue? |
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Definition
Phenol kills orgaisms
Lactic acid preserves fingal structures
Cotton blue stains the chitin in cell walls |
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Term
| How are fungal specimens cultured? |
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Definition
30C at 40-50% humidity
Keep all cultures at least a month |
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Term
| When is rice grain agar used? |
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Definition
To differentiate Microsporum audouinii from M. canis
M. audouinii grows poorly |
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Term
| Which media is good for slide culture? |
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Definition
| Potato dextrose agar becuase it induces sporulation |
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Term
| What stain is used to distinguish Cryptococcus? |
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Definition
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Term
| What fungal stains are used on tissues? |
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Definition
| GMS, PAS, Mucicarmine stain, Papanicolaou stain and the Wright-Giemsa stain |
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Term
| What is the advantage of adding cycloheximide to agar? |
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Definition
| Inhibits fungus like bacteria, many yeasts and many fungal opportunists |
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Term
| What is the advantage of adding gentamicin to agar? |
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Definition
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Term
| What is the advantage of adding chloramphenicol to agar? |
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Definition
| Inhibits fungus like bacteria and yeast phases of dimorphic fungi |
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Term
| When is mycosel agar used? |
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Definition
Isolation of pathogenic fungi from contaminated specimens
Most opportunistics wont grow - has chloramphenicol and cycloheximide |
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Term
| What is a tease mount and what is a disadvantage of performing it? |
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Definition
Teasing needles are used to remove mycelium
It disrupts conidia |
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Term
| What stain is used with a cellophane tape prep and what are the advantages and disadvantages? |
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Definition
Lactophenol cotton blue
Conidia arrangment is maintained
Potential for contamination |
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Term
| What structures does Candida albicans produce on cornmeal agar with Tween? |
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Definition
Hyphae, pseudohyphae, blastoconidia and chlamydoconidia
Cryptococcus does not produce hyphae |
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