Term
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Definition
| Bacteria can create a restriction enzyme for cleaving DNA at a specific sequence called a restriction site. |
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Term
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Definition
| Specific to the restriction enzyme. Usually Palindromic (RACECAR) |
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Term
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Definition
| In certain cases two or more different enzymes cleave at the same point. |
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Term
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Definition
| Overhung ends that want to join together with complementary base pairs. |
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Term
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Definition
| done by topographical differences. Will be circular while chromosomal DNA is linear. When ethidium bromide binds it leads to different densities. |
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Term
| PCR - The polymerase chain reaction |
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Definition
| raise temp to 97 which makes DNA single stranded. lower temp to 55 where primers are binded (comp. to 5' ends of DNA). raise temp to 72 degrees where the taq polymerase replicates the DNA. |
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Term
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Definition
G1 - is my DNA damaged?
G2 - is my DNA replicated
Miotic Checkpoint - is my DNA aligned with spindles |
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Term
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Definition
| Programmed Cell death: happens for development (fingers, toes, nerves, homeostasis) and to kill cells that are a threat. |
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Term
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Definition
| 2 categories: Initiators(begin the process by initiation cascade, and executioners that actually kill cell) |
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