Term
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Definition
Use a bulb
Aspirate liquid past meniscus
Wipe tip
Bring bottom of liquid meniscus to desired gradation line
Aspirate as indicated |
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Term
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Definition
| Graduated to the tip and has a double ring. Must be dispensed all the way. Known as serologic pipettes used to transfer reagents |
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Term
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Definition
| Has single ring. Not graduated to the tip. Do not blow out. |
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Term
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Definition
| Used for calibrators and QC. Not blow out. Used to dispense a single volume of liquid |
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Term
| If you dilute a patient's sample what must you do after obtaining their final protein result? |
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Definition
| Multiply it by the dilution factor. |
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Term
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Definition
| Contains no anticoagulant. Used with serum |
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Term
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Definition
| Contains anticoagulant. Used with plasma. |
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Term
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Definition
| Anticoagulant. Contains fibrinogen. |
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Term
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Definition
| Clear sample contains no fibrinogen |
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Term
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Definition
| Red blood cells are broken. Sample appears deep red if whole blood, pink/red if serum. |
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Term
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Definition
| Sample contains high amount of bilirubin. Sample will appear to have an orange tinge. |
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Term
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Definition
| Sample contains high amount of fat. Sample appears turbid. |
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Term
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Definition
| Sample contains a high amount of protein/fibrin. |
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Term
| In the total protein method using buiret, the peptide bond in proteins will bind what? |
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Definition
| Cupric ions in an alkaline pH to form a blue/purple complex. |
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Term
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Definition
| Consists of 100ul H20. 4.9ml NaoH and ml Buiret. Used to take only the absorbance of the reagents and blank out the color or the patient sample |
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Term
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Definition
| Consists of 100ul patient sample and 5.9ml H20. Used to take the absorbance of only the patient sample and blank out the color of the reagent |
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Term
| What must be done with the absorbance reading of a sample blank? |
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Definition
| It must be subtracted from the absorbance reading of the patient after both readings have been obtained. |
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Term
| Dilution factors are written as |
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Definition
| Stock:Total parts (Stock in total parts) |
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Term
| "1 to 2" dilution would be |
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Definition
| 1 part stock to 2 parts diluent |
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Term
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Definition
Wavelength accuracy-Can you set a specific wavelength
Photometric accuracy- Tests the light source and photo dtector
Stray light |
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Term
| How do you perform a wavelength check? |
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Definition
| Have a known maxima/wavelength and either use a filter (didymium, holmium oxide or solutions) or do a color check in which you check the color coming from the monochromator. |
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Term
| How do you perform a photometric accuracy check? |
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Definition
| Test absorbance at known maxima. Beer's law cqalculated versus measured. Linearity. Dilutions |
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Term
| The photometric accuracy check checks what? |
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Definition
| Light source, cuvette, photodetector predictable response |
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Term
| What is the part of the spec check that must always be performed first? |
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Definition
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Term
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Definition
| Extraneous light hitting the photodetector. Interpreted as too much light being transmitted. Will cause the line on a graph to be flatter |
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Term
| Where are the best wavelengths to measure stray light and the best way to measure it? |
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Definition
| 400 and 700nm and measure %T. Block the light path and the reading should be zero. |
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Term
| Cut-off filters or solutions |
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Definition
| More accurate way to measure stray light. Above a certain absorbance they don't transmit any light. |
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Term
Wavelength check is measured in?
Photometric accuracy check is measured in? |
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Definition
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Term
| When do you need a 1 point cal? |
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Definition
| Every 6 months it is required by federal legislation for every assay that reports patient patient results or anytime something changes in the system |
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Term
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Definition
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Term
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Definition
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Term
| Absorptivity constant (L mol^-1 cm^-1 for molar absorptivity) |
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Definition
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Term
| Sample light path (Cuvette width) |
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Definition
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Term
| Concentration of absorbing material |
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Definition
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Term
| 1-Point Calibration Formula |
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Definition
| Known concentration/Known Absorbance = Unknown concentration/Unknown Absorbance |
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Term
| What is the protein reference range for adults? |
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Definition
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Term
| At what wavelength should the absorbance be measured during a total protein test using the biuret method? |
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Definition
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Term
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Definition
| Increased production of protein. Caused by multiply myeloma, dehydration, etc |
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Term
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Definition
| Loss of protein. Caused by burns, nephrotic syndrome, bleeding, etc |
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