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| Best temp for S. marcenscense |
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Definition
| 20 degrees C (mesophil)- red pigment |
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| 3 toxic Oxygen forms (superoxides) |
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Definition
Superoxide
Peroxide
Hydroxyl |
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| 3 enzymes for superoxide breakdown. Where are they found? |
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Definition
Found in aerobes
- Superoxide dismutase
-Catalase
-Peroxidase |
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| What agar medium was used in the oxygen requirement experiments? |
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Definition
| Brain heart fusions in deep tubes |
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| What area in the agar do aerobes grow? |
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Definition
| at the surface of the tube |
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| what area in the agar do obligate anaerobes grow? |
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Definition
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| what area in the agar do facultative anaerobes grow? |
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Definition
| throughout the agar with more at the surface |
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| What is the final electron acceptor for aerobes? |
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Definition
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| what is the final elector acceptor for obligate anaerobes? |
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Definition
| molecules other than oxygen |
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| In the catalase experiment, what solution is added to the tubes |
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| What represents a positive reaction in the catalase test? |
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Definition
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| in a normal cell, where would the hydrogen peroxide being neutralized by catalase come from? |
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Definition
| superoxide dismutase breaks down toxic superoxides and produces hydrogen peroxide. |
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| What agar and reagent is used in the amylase test? |
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Definition
| starch agar and Gram's iodine |
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| What reaction is being tested for in the amylase test? |
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Definition
| Starch hydrolysis into simple sugars (glucose) |
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| What does a positive Amylase test looks like (with Gram's iodine added)? |
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Definition
| cleared zone (halo) surrounding the growth |
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| What does a negative Amylase test looks like (with Gram's iodine added)? |
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Definition
| Blue to black color in the medium |
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| What agar is used in the lipase test? |
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Definition
| Tributyrin agar (with lipid)- blue color |
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| What reaction is being tested in the lipase test? |
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Definition
| hydrolysis of ester bonds by lipase |
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| What does a positive lipase test look like? |
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Definition
| clear halo surrounding growth. Loss of apacity |
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| What does a negative lipase test look like? |
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Definition
| Medium remains opaque blue color surrounding growth |
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| What media is used in the Casein test? |
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Definition
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| What reaction is being tested in the Casein test? |
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Definition
| breakdown of casein, a protein in milk, by protease |
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| What does a positive results in the Casein test look like? |
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Definition
| loss of opacity surrounding the bacterial growth |
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| What does a negative result in the casein test look like? |
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Definition
| medium surrounding the bacterial growth remains opaque |
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| What agar and broth was used for the temperature experiment? |
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Definition
| Nutrient broth and TSA platse |
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| What method was used to inoculate the oxygen requirement bacteria in brain hear fusion? |
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Definition
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| What are the 4 exoenzymes? |
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Definition
1. lipase
2. amylase
3. gelatin
4. casein |
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| in the gelatin experiment, what enzyme is breaking down gelatin and what is it breaking gelatin down into? |
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Definition
| gelatinase breaks gelatin down into amino acids |
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| how many ml to one micro liter? |
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| In the DNA extraction experiment, what is done to the strawberry cells to expose the DNA? |
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Definition
| The cells are treated with detergent (SDS) in a buffer solution |
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| In the DNA extraction experiment, how is protein precipitated out of the solution? |
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Definition
| presence of salt in the DNA extraction buffer |
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| In the DNA extraction experiment, how is cellular waste and particles removed from solution? |
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| In the DNA extraction experiment, how is DNA precipitated from the filtered solution? |
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| Transformation- What two genes were present in the plasmid vector? |
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| Transformation- how did we select for transformed bacteria? |
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Definition
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| Transformation- what was done to E.coli to induce competency? |
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Definition
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| Transformation- what were the growth areas surrounding transformed bacteria called? |
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| What is another name for the disk diffusion test (antibiotics)? |
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Definition
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| What is the area surrounding an antibiotic disk that restricts growth called? |
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Definition
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| What are two typical gram + bacteria? |
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Definition
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| What three sugars do phenol red tubes contain for carbohydrate fermentation? |
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Definition
1. glucose
2. lactose (galactose + glucose)
3. sucrose (fructose + glucose) |
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| In carbohydrate fermentation, which bacteria usually produces acid? |
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Definition
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| In carbohydrate fermentation, which bacteria doesn't undergo gas or acid production? |
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Definition
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