Term
| What is the process of destroying the native conformation of a protein by chemical or physical means? |
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Definition
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Term
| What is denaturation and is it reversible |
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Definition
| Pocess of destroying the native conformation of a protein by chemical or physical means and can or cannot be reversible |
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Term
| Name an irreversible denaturing agent |
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Definition
| Heat-disrupts hydrogen bonding and precipitation may take place |
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Term
| Name two ways to denature a protein tha is reversible? |
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Definition
| 6M aqueous urea; alcohols-70% ethanol disrupts hydrogen bonding |
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Term
| What ions form water-insoluble salts with SH groups when denaturing a protein? |
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Definition
| Heavy metal ions Pb2+, Hg2+, and Cd2+ |
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Term
| Which reducing agents form reversible denaturizing reactions with proteins? |
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Definition
| 2-mercaptoethanol cleaes disulfide bonds by reducing s-s groups to s-H groups |
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Term
| Do detergents denature proteins reversibly? |
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Definition
| Yes, surfact-active agents sodium dedecylulfate, sds distupt hydrogen bonding |
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