Shared Flashcard Set

Details

Title

Protein Sequencing

Description

Review of Sanger Sequencing and Edman Degradation

Total Cards

Subject

Biochemistry

Level

Undergraduate 3

Created

09/28/2026

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Additional Biochemistry Flashcards

Term

Sanger Sequencing:

-Reagent

-What can be deduced

Definition

-FDNB

-Marks N-terminus (left)

Term

Sequencing Reagent:

Trypsin

Definition

-Cleaves at K and R

Term

Sequencing Reagent:

Chymotrypsin

Definition

-Cleaves at aromatic AA's (F, W, Y)

Term

Sequencing Reagent:

Cyanogen Bromide

Definition

-Cleaves at M