Term
What does the acronym CRISPR stand for?
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Definition
Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. |
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Term
What biological research areas have benefitted from the CRISPR/Cas9 technology?
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Definition
development of model cell lines
discovering mechanisms of disease
identifying disease targets
development of transgene animals and plants
transcriptional modulation |
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Term
Who is the scientist credited with discovering the "Cas" portion of the CRISPER/Cas gene editing tool, and in what year was this advance made.
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Definition
In 2002 Ruud Jansen discovered a set of genes adjacent to the CRISPR locus, which was termed CRISPR-associated system, or Cas
Ruud Jansen, PhD
Medical Molecular Microbiologist
Streeklaboratorium voor de Volksgezondheid Kennemerland
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Term
What is the biological mechanism of
the adaptive immune system as it relates to the CRISPR function?
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Definition
CRISPR functions as a genomic memory of invading pathogens. This memory is used by Cas proteins, serving as guided endonucleases, to scan for invading DNA and disable it by introducing DSBs |
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Term
The CRISPR/Cas9 system locus in comprised of three components, please name them.
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Definition
| The genomic CRISPR locus is comprised of three components: the trans-activating CRISPR RNA (tracrRNA) gene, the Cas gene, and the CRISPR repeat and spacer sequences |
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