Term
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Definition
| Personal Protective Equipment. Consists of lab coats, goggles, closed toed shoes, gloves, long pants, washing your hands, no contacts. |
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Term
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Definition
| Baby pink. Selects for Gram negative bacteria, Gram positive inhibited by crystal violet and bile salts,Lactose fermenters will turn pink on the agar because products of fermentation are acidic. |
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| Mannitol Salt agar (MSA plate) |
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Definition
| Hot pink, Selects for Gram positive bacteria, Gram negatives do not grow, turns yellow when salt ferments, selective for staph because of its high salt content. |
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Term
| Eosin Methylene Blue Agar (EMB plate) |
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Definition
| Red, Selects for Gram negative bacteria (especially Coliform),Low pH inhibits gram positive, Vigorous lactose fermentation=metallic green (E. coli), normal lactose fermentation=dark purple, weak fermentation=pink |
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Term
| Phenylethyl Alcohol Agar (PEA plate) |
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Definition
| White,Selects for Gram positive bacteria, Gram negative inhibited by phenylethyl alcohol which interrupts DNA synthesis |
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Term
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Definition
| Medium used to test differences in oxygen utilization, Obligate aerobes will only grow in this oxygen-rich top layer, Obligate anaerobes will only grow in the lower areas of the tube, Microaerophiles will grow in a thin layer below the richly-oxygenated layer, |
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Term
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Definition
| Jar has sachet added to remove O2 from jar, anaerobic bacteria grows, aerobic does not. |
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Term
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Definition
| Spherical shaped bacteria, group in bunches. EX: Staph. |
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Term
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Definition
| Rod shaped bacteria, long chains, EX: Strept. |
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Term
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Definition
| Like oxygen. grow at top of tubes. |
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Term
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Definition
| Don't like oxygen, grow at bottom of tube. |
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Term
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Definition
| Like oxygen. Grow directly beneath the surface. |
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Term
| Facultative or aerotolerant |
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Definition
| Don't like or dislike oxygen. Grow throughout or in the middle. |
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Definition
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Definition
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Term
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Definition
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Term
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Definition
| Sterilization for aseptic technique. |
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Term
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Definition
| Dry the slide when gram staining |
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Term
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Definition
| The bacteria has a thick cell wall. Purple. |
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Term
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Definition
| Bacteria has a thick outer membrane or lipid layer. Pink/Red. |
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Term
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Definition
| 10x magnification. The lens you look through. |
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Term
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Definition
| Connection area for parts of the microscope. Where you grab when carrying a microscope. |
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Term
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Definition
| Part of the microscope that allows the objective lenses movement. |
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Term
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Definition
| 4x,10x,40x,100x. The lens closest to the slide that turns it into a 3-D image. |
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Term
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Definition
| Holds the slide in place on the stage. |
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Term
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Definition
| Where the slides lie on for observation |
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Term
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Definition
| Has different sized adjustable holes in order to control the amount of light that comes through. |
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Term
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Definition
| Focuses the light on the slide. |
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Term
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Definition
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Term
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Definition
| Bottom of the microscope. Where you hold when carrying a microscope. |
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Term
| Mechanical Stage Adjustment Knob |
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Definition
| Moves the stage side to side or forward and back. |
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Term
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Definition
| Moves objective lens toward or away from the slide. |
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Term
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Definition
| Used for fine tuning the focus on the specimen or to focus on a specific part of the specimen. |
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Term
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Definition
| Controls the brightness of the light coming from the lamp. |
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Term
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Definition
| Turns the lamp on or off. |
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Term
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Definition
| Used to look at specimens on slides. |
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Term
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Definition
| Safety procedures done in order to prevent the spread of infection. |
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Term
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Definition
| Used to add contrast in order to see the specimens that are otherwise naked to the human eye. |
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Term
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Definition
| Used t see the bacteria. Uses Safranin in order to color the bacteria and leave a white background. |
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Term
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Definition
| Uses a black/dark background and keeps the bacteria white/transparent. |
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Term
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Definition
| Uses multiple stains (Crystal Violet, Iodine, and Safranin) in order to determine gram negative and gram positive bacteria in a specimen. |
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Term
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Definition
| Red/Pink biological stain that stains gram negative cells. |
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Term
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Definition
| Purple biological stain that stains gram positive cells. Gram positive=purple, gram negative=pink/red. |
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Term
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Definition
| Fixes the stain to the cell. |
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