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| the purpose of the In-Vitro Sperm Penetration lab |
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| This laboratory exercise is designed to demonstrate the ability of avian spermatozoa to penetrate the vitelline membrane of oviposited eggs. This assay serves as a relative means of accessing reproductive potential in breeding stocks of poultry. |
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| how to get the albumen off of the yolk |
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| 2. Crack open an egg and separate the ovum (yolk) from the albumen using a yolk separator. Wash albumen from the yolk by gently rinsing with 1% NaCl while the yolk is still on the separator |
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| how to get adhering albumen off of the yolk |
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| 3. Gently place the ovum (yolk) on a paper towel moistened with 1% NaCl and roll it across the paper towel to remove adhering albumen. |
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| this was used to stain the vitelline membrane purple |
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Definition
| Schiff’s Reagent + 10% Formalin => Purple Stain Glycoprotein |
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| The area of the vitelline membrane that covers the germinal disc will appear as... |
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| a "donut" and spermatozoa will generate holes in a circular pattern that surrounds this donut. |
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| depiction of chicken sperm meeting yolk |
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| what does the acrosome do? |
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| secretes Hydrolytic Enzymes that Digest Hole in Vitelline membrane |
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