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PMIBO Quiz 1
PMIBO Quiz 1
447
Microbiology
Undergraduate 3
08/24/2020

Additional Microbiology Flashcards

 


 

Cards

Term
Microbiology
Definition
Study of living things that are too small to be seen without magnification
Term
the 3 super kingdoms in the Woese-Fox classification system
Definition
-Eubacteria
-Archaebacteria
-Eukarya
Term
the Woese-Fox classification system classifies organisms into super kingdoms based on...
Definition
rRNA
Term
the Woese-Fox classification system
Definition
classification of organisms into the super kingdoms Eubacteria, Archaebacteria, and Eukarya based on rRNA
[image]
Term
the 5 kingdoms in the Whittaker classification system
Definition
-Prokaryote (Monera)
-Protista
-Fungae
-Plantae
-Animalia
Term
the Whittaker classification system classifies organisms into kingdoms based on...
Definition
Morphology, metabolism, & molecular techniques
Term
some molecular techniques used to classify organisms in the Whittaker classification system
Definition
-fatty acid profiles
-protein differentiation
-DNA fingerprinting
Term
the Whittaker classification system
Definition
classifies organisms into the kingdoms Prokaryote (Monera), Protista, Fungae, Plantae, and Animalia based on Morphology, metabolism & molecular techniques
[image]
Term
Carolus Linnaeus
Definition
Developed the taxonomic system for naming plants and animals
Term
Leeuwenhoek’s system of classification
Definition
-Fungi
-Protozoa
-Algae
-Bacteria
-Archaea
-Small animals
Term
Taxa (Taxon)
Definition
-Designed to show degrees of similarity among organisms (Groups based on similarity)
-Higher taxa - very general
-Lower taxa - more restricted
Term
Higher taxa
Definition
very general
Term
Lower taxa
Definition
more restricted
Term
In general, we talk at the ______ level for microorganisms
Definition
genus and species
examples:
Escherichia coli
Salmonella enterica
Campylobacter jejune
Term
the taxonomic hierarchy for microbes
Definition
-domain
-phylum
-class
-order
-genus
-species
[image]
Term
some characteristics of fungi
Definition
-Eukaryotic (have membrane-bound nucleus)
-Obtain food from other organisms
-Possess cell walls
Term
some types of fungi
Definition
-mold
-yeast
Term
some characteristics of mold
Definition
-multicellular
-have hyphae
-reproduce by sexual and asexual spores
Term
some characteristics of yeast
Definition
-unicellular
-reproduce asexually by budding
-some produce sexual spores
Term
Pseudopodia
Definition
cell extensions that flow in direction of travel
Term
Cilia
Definition
numerous, short, hairlike protrusions that propel organisms through environment
Term
Flagella
Definition
extensions of a cell that are fewer, longer, and more whiplike than cilia
Term
some characteristics of algae
Definition
-Unicellular or multicellular
-Photosynthetic
-Simple reproductive structures
-Categorized on the basis of pigmentation, storage products, and composition of cell wall
Term
algae are categorized on the basis of...
Definition
-pigmentation
-storage products
-composition of cell wall
Term
some characteristics of Bacteria and Archaea
Definition
-Unicellular and lack nuclei
-Much smaller than eukaryotes
-Found everywhere there is sufficient moisture; some found in extreme environments
-Reproduce asexually
Term
how bacteria differ from archaea
Definition
cell walls contain peptidoglycan; some lack cell walls; most do not cause disease and some are beneficial
Term
how archaea differ from bacteria
Definition
cell walls composed of polymers other than peptidoglycan
Term
how bacteria are named
Definition
-Generic (genus) followed by species -Generic part is capitalized, with species in lower case -Both are italicized or underlined -E.g. Listeria monocytogenes
Term
[image]
Definition
Haeckel’s Phylogenetic Tree (1866)
Term
[image]
Definition
Universal phylogenetic tree
Term
the universal phylogenetic tree is based on...
Definition
rRNA sequence
Term
how evolution occurs
Definition
Hereditary information in living organisms changes gradually through time – these changes result in structural and functional changes through many generations
Term
some preconceptions of origin and evolution
Definition
-All new species originate from pre-existing species
-Closely related organisms have similar features because they evolved form a common ancestor
Term
how bacteria are affixed to a slide
Definition
heat
Term
Hans Christian Gram
Definition
-1853–1938
-developed Gram staining
Term
what's number 1?
[image]
Definition
negative
Term
what's number 2?
[image]
Definition
positive
Term
what's number 1?
[image]
Definition
mesosome
Term
what's number 2?
[image]
Definition
peptidoglycan layer
Term
what's number 3?
[image]
Definition
(capsule)
Term
what's number 4?
[image]
Definition
inclusion body
Term
what's number 5?
[image]
Definition
cytoplasmic membrane
Term
what's number 6?
[image]
Definition
(flagellum)
Term
what's number 7?
[image]
Definition
surface proteins
Term
what's number 8?
[image]
Definition
ribosome
Term
what's number 9?
[image]
Definition
chromosome
Term
what's number 10?
[image]
Definition
ribosome
Term
what's number 11?
[image]
Definition
(flagellum)
Term
what's number 12?
[image]
Definition
periplasmic space
Term
what's number 13?
[image]
Definition
porin proteins
Term
what's number 14?
[image]
Definition
peptidoglycan layer
Term
what's number 15?
[image]
Definition
inclusion body
Term
what's number 16?
[image]
Definition
cytoplasmic membrane
Term
what's number 17?
[image]
Definition
(Capsule)
Term
what's number 18?
[image]
Definition
outer membrane
Term
what's number 19?
[image]
Definition
(Pili)
Term
what's number 20?
[image]
Definition
division septum
Term
the structure of a Gram negative cell wall
Definition
[image]
Term
the structure of a Gram positive cell wall
Definition
[image]
Term
structure of peptidoglycan
Definition
[image]
Term
some geotypic means of bacterial classification
Definition
-Universal phylogenetic tree (16s rRNA)
-Ribosomal RNA sequence analysis
-Molecular sub-typing
Term
the microbial taxa found in poultry
Definition
[image]
Term
some categories of sources of meat contamination in poultry
Definition
-Growth
-Transportation
-Processing
-Post-processing
Term
which method of exposure causes faster Salmonella enteritidis colonozation in the internal organs of chickens? oral inoculation or contact?
Definition
oral inoculation
Term
Natural microflora of poultry can be from...
Definition
-Environment
-Gastrointestinal contents
-Air in the processing
-Personnel in production and processing operations
Term
some types of natural microflora
Definition
-Spoilage organisms
-Pathogenic organisms
Term
some sources of contamination in fresh poultry or food products
Definition
-Majority from the growing environment
-Gastrointestinal contents
Term
some sources of contamination in processed poultry or food products
Definition
-Ingredients
-Environmental contamination (equipment, personnel or air)
-Ingredients added after processing (e.g., spices added to the chips after frying)
Term
some pre-harvest sources of contamination of poultry
Definition
-Feces
-Soil
-Water (feeding and irrigation)
-Air
-Dust
-Transportation cages & vehicles
-Packaging equipment
-Cross contamination
Term
some waterborne sources of pre-harvest contamination of poultry
Definition
-feeding
-irrigation
Term
the major digestive and absorbing organ
Definition
Gastrointestinal tract
Term
where in the chicken's digestive tract is the microflora most extensive?
Definition
the cecum
Term
why chicks need microflora in their ceca
Definition
because absence of microflora in the cecum is considered a major factor in susceptibility of chicks to bacterial infection
Term
a major factor in susceptibility of chicks to bacterial infection
Definition
absence of microflora in the cecum
Term
order of microbial succession in poultry feces
Definition
-3-6 h after hatching: anaerobic bacteria that can decompose uric acid (cecal microflora)
-2-4 d post-hatch: Streptococci and Enterobacteria (small intestine and cecum)
-After 1st week: Lactobacilli in small intestine and anaerobes (Escherichia coli and Bacteroides) and smaller number of facultative aerobes
-2 weeks: typical microflora established
-30 d: adult cecal flora (obligate anaerobes) established; Bifidobacteria and Bacteroides predominate
Term
the microbes in poultry feces 3-6 h after hatching
Definition
anaerobic bacteria that can decompose uric acid (cecal microflora)
Term
microbes in poultry feces 2-4 d post-hatch
Definition
Streptococci and Enterobacteria (small intestine and cecum)
Term
microbes in poultry feces 1 week after hatching
Definition
Lactobacilli in small intestine and anaerobes (Escherichia coli and Bacteroides) and smaller number of facultative aerobes
Term
how the microbiome changes in an incubating egg
Definition
[image]
Term
the microbes in poultry feces 2 weeks after hatching
Definition
typical microflora established
Term
the microbes in poultry feces 30 days after hatching
Definition
adult cecal flora (obligate anaerobes) established; Bifidobacteria and Bacteroides predominate
Term
the bacteria in poultry feces by age
Definition
[image]
Term
Salmonella important for which poultry product?
Definition
eggs
Term
regulations on foodborne pathogens are important for...
Definition
processing and products, such as meat and eggs
Term
disease related to COVID-19
Definition
Middle East Respiratory Syndrome (MERS)
Term
where Middle East Respiratory Syndrome (MERS) was most prevalent and why
Definition
in the MidEast because of camels
Term
some sausages are fermented to lower...
Definition
pH
Term
one things bacteria have that archaeans possibly don't have
Definition
cell wall
Term
______ are “true bacteria”
Definition
Eubacteria
Term
why rRNA is used to classify organisms
Definition
because it is the most conserved; it is the genetic material that changes the least
Term
why bacteria form spores
Definition
because of conditions not suitable for growth
Term
what happens to the genetic material when bacteria sporulate?
Definition
it condenses
Term
the only cells that can grow and divide
Definition
Vegetative cells
Term
After ______, there were envelopes containing anthrax (Bacillus anthracis), which is spore-forming
Definition
9/11
Term
After 9/11, there were envelopes containing...
Definition
anthrax (Bacillus anthracis), which is spore-forming
Term
some spore-forming bacteria
Definition
-Bacillus anthracis -Clostridium botulinum
Term
Clostridium botulinum produces...
Definition
botulism toxin, which is a very potent neurotoxin
Term
some things Clostridium botulinum spores can survive
Definition
boiling for 5 minutes, UV light for several hours, and hydrogen peroxide for a few minutes
Term
Protozoa
Definition
single-celled eukaryotes
Term
why water should be boiled when camping
Definition
Giardia, which is a protozoan
Term
a parasite of concern with swine
Definition
Trichinella spiralis
Term
why pork should be cooked so thoroughly
Definition
because of Trichinella spiralis in the muscles
Term
how Trichinella spiralis gets into the pig's muscles
Definition
swine can eat rodents, which carry it, and the parasite can translocate into the swine’s muscles
Term
some microbes in poultry litter that can cause pre-harvest contamination
Definition
-aerobic bacteria -enterics, such as Enterococcus spp., Coliforms, and Salmonella spp.
Term
some potential pathogenic bacteria that can be in poultry litter
Definition
-Enterococcus spp. -Coliforms -Salmonella spp. -Clostridium -Staphylococcus -Facklamia -Bordetella -Brevibacterium -Vagococcus -Campylobacter -Yersinia -Listeria
Term
the water usually used for poultry feeding
Definition
potable water
Term
the water usually used for poultry processing
Definition
potable water
Term
what potable water is used for
Definition
-poultry feeding
-poultry processing
Term
the water cycle
Definition
[image]
Term
the steps of water treatment
Definition
1: Flocculation/sedimentation
2: Filtration
3: Ion exchange
4: Absorption
5: Disinfection (chlorination/ozonation)
Term
Flocculation/sedimentation
Definition
alum used to remove iron salts or synthetic organic polymers (verify with Dr. Thippareddi)
Term
Filtration
Definition
uses clays and silts to remove natural organic matter, other precipitates (iron and manganese); enhances effectiveness of disinfection
Term
Ion exchange
Definition
-using ions to remove inorganic contaminants that are not removed by filtration & sedimentation
-using ions to treat hard water (remove mineral contaminants)
-ions used to remove arsenic, chromium, excess fluoride, nitrates, radium, and uranium
Term
Absorption
Definition
organic contaminants, unwanted coloring and odorous compounds are removed (using activated carbon)
Term
Disinfection (chlorination/ozonation)
Definition
-Remove microorganisms - foodborne pathogens
-Outbreaks – boil water advisory
Term
the water treatment process
Definition
[image]
Term
some National Primary Drinking Water Regulations
Definition
-Cryptosporidium, Giardia lamblia, viruses (enteric), Heterotrophic Plate Count (HPC), Legionella, Total Coliforms (<5%; including fecal coliforms and E. coli) -Turbidity
Term
place in Canada where there has been a waterborne disease outbreak
Definition
Walkerton, Ontario
Term
some details about the water contamination in Walkerton, Ontario
Definition
-this water came from an underground well that may have been contaminated by E. coli in cattle feces -3 samples from the construction site are positive for total and fecal coliforms (E. coli) -other sample suspect -Boil Water Notice -Ilnesses (2,300), deaths (7), several others suffer permanent organ damage
Term
some places in the USA that have had waterborne disease outbreaks
Definition
-Milwaukee, Wisconsin
-Cabool, Missouri
-New York State Fairgrounds
Term
who reports Waterborne Disease Incidents and Investigations?
Definition
CDC
Term
maximum acceptable levels of Total Heterotrophic Bacteria in pre-harvest poultry drinking water recommended in the Poultry Drinking Water Primer (developed at UGA Poultry Science Department)?
Definition
100 CFU/100 mL
Term
maximum acceptable levels of Coliform Bacteria in pre-harvest poultry drinking water recommended in the Poultry Drinking Water Primer (developed at UGA Poultry Science Department)?
Definition
50 CFU/100 mL
Term
some organisms of concern in pre-harvest poultry air
Definition
-anaerobes -aerobes -lactics -molds -S. aureus -E. coli
Term
electrostatic charge system
Definition
a way to reduce the amount of pathogens, ammonia, and dust in broiler house air
Term
computational fluid dynamics simulations of airborne dispersion
Definition
a way to predict the spread of highly pathogenic avian influenza using a multifactor network
Term
Biosecurity
Definition
Procedures to prevent introduction and spread of disease-causing organisms in poultry flocks
Term
some pathogens transmitted to chickens during transport to slaughter
Definition
-Campylobacter spp. -Coliform -E. coli
Term
how Campylobacter spp., Coliform, Salmonella, and E. coli may be transmitted to chickens during transport to slaughter
Definition
-cross-contamination occurring in dirty transportation coops/crates
-feces from birds in higher crates can get onto birds in lower crates
Term
which depth of turkey skin tends to have the highest levels of Salmonella typhimurium?
Definition
0-50 μm
Term
a type of wash that may reduce pathogen levels in poultry processing
Definition
spray wash
Term
how to minimize the contamination in poultry
Definition
Manage the potential sources of contamination by...
-Proper management of the house
-Proper management of the air
-Biosecurity
Term
how to manage the potential sources of contamination
Definition
-Proper management of the house
-Proper management of the air
-Biosecurity
Term
is the intact muscle of a healthy food animal sterile?
Definition
evidently not; it can contain zoonotic organisms
Term
zoonotic organisms
Definition
organisms that cause disease in humans, but not animals
Term
some examples of zoonotic organisms
Definition
-Salmonella -Campylobacter
Term
some organs Salmonella enteridis usually does not translocate into
Definition
-ovary
-oviduct
Term
some organs other types of Salmonella can often translocate into
Definition
-liver
-spleen
Term
a recent example of an outbreak of a Salmonella outbreak caused by undercooked chicken liver
Definition
several outbreaks of salmonellosis related to liver pate
Term
most of the natural microflora in a chicken is from...
Definition
its gastrointestinal tract
Term
is it possible to completely avoid cross-contaminating chicken meat with GI contents during processing?
Definition
no
Term
spoilage organisms
Definition
the ones that reduce the shelf life of the product
Term
pathogenic organisms
Definition
the ones that cause disease
Term
an example of a foodborne pathogen that contaminates a product after cooking
Definition
Listeria monocytogenes; can be on the equipment if it's not sanitized properly
Term
example of outbreak of disease caused by eating processed food
Definition
consumption of chips that have Salmonella
Term
why different parts of the GI tract have different types of microbes
Definition
because of different conditions
Term
why the cecum has the greatest diversity of microflors
Definition
because it's a dead end/blind pouch; lots of nutrients end up there
Term
microbial succession
Definition
the types of microbes in the bird's GI tract change over time as it gets older
Term
Bifidobacteria are in which group of bacteria?
Definition
lactic acid-producing bacteria
Term
today, we can look more closely at the bacterial species because of...
Definition
-genetics
-PCR
Term
types of microflora that change over time
Definition
-E. coli -Salmonella
Term
types of microflora that are pretty stable over time
Definition
-lactic acid bacteria -Bifidobacteria
Term
when levels of Salmonella in the bird are highest
Definition
day 4
Term
after day 4, levels of Salmonella in the bird decline as the bird...
Definition
develops immunity
Term
what method is being used to analyze this microflora ecology?
[image]
Definition
using 16S RNA primers
Term
after hatching, when are the Lactobacilli established?
Definition
by day 7
Term
the major contributor of microbial diversity in the litter of a poultry house
Definition
feces
Term
CFU
Definition
colony-forming units
Term
the amount of CFU's of total aerobic bacteria that can be found in the litter in a chicken house
Definition
~109 CFU's
Term
the most common type of enteric in poultry house litter
Definition
Enterococcus spp.
Term
potable water
Definition
water that's been treated such that it's suitable for consumption by humans
Term
when there's a boil water advisory
Definition
during outbreaks
Term
some pathogens drinking water is required to be almost free of
Definition
-E. coli -Legionella
Term
one way pathogens can become airborne in a poultry house
Definition
dust gets kicked up from the litter when the litter is dry
Term
why is the air more contaminated in a poultry house that has more birds?
Definition
because more dust, which can carry pathogens, is generated
Term
how poor air quality harms birds
Definition
pathogens get into the respiratory tract and causes illness and such
Term
which type of floor in poultry houses results in better air quality? litter floor or sloping wire floor?
Definition
sloping wire floor
Term
why sloping wire floors result in better air quality in poultry houses
Definition
because the poop drops down thru the wire, resulting in not as much pathogen-containing dust being kicked up
Term
some diseases of concern in biosecurity with poultry
Definition
-highly pathogenic avian influenza (HPAI)
-avian influenza
-Newcastle disease
Term
HPAI
Definition
highly pathogenic avian influenza
Term
what does it mean to maximize the environment in a poultry house?
Definition
to maintain dry litter and good ventilation
Term
how to reduce the transmission of diseases by way of transportation crates
Definition
-wash them to get rid of the organic matter of the feces
-use the right sanitizers to kill the bacteria that are present there
-dry the crates so the organisms can't survive very long
Term
how to get rid of pathogens that are in the bird's skin
Definition
either by cooking or high-pressure processing
Term
how pathogens get into the bird's skin
Definition
they get embedded into the pores or capillaries on the skin or the feather follicles during a defeathering process
Term
will sanitizers kill pathogens that are in the pores, capillaries, or feather follicles on the bird's skin?
Definition
no
Term
the skin on an uncooked bird can be a source of...
Definition
-Salmonella -Campylobacter
Term
why leaving the skin on a piece of bird meat reduces the shelf life of the product
Definition
because the skin is a major source of microorganisms, including those that cause spoilage
Term
how to manage the poultry house to minimize contamination
Definition
biosecurity
Term
how to manage the air in a poultry house to minimize contamination
Definition
keep the litter dry; this reduces the risk of Campylobacter and other organisms surviving
Term
how to manage the potential sources of contamination for poultry
Definition
-Proper management of the house
-Proper management of the air
-Biosecurity
Term
Name the five kingdoms in classification of microorganisms and what is the basis for the classification?
Definition
the 5 kingdoms: Prokaryote (Monera), Protista, Fungae, Plantae, Animalia

the basis: Morphology, metabolism, & molecular techniques
Term
What are the main differences between yeasts and molds?
Definition
[image]
yeasts: unicellular, reproduce asexually by budding, some produce sexual spores

molds: multicellular, have hyphae, reproduce by sexual and asexual spores
Term
What are the features of protozoa?
Definition
-Single-celled eukaryotes
-Similar to animals in nutrient needs and cellular structure
-Live freely in water; some live in animal hosts
-Asexual (most) and sexual reproduction
-Most are capable of locomotion by pseudopodia, cilia, and flagella
Term
example of a protozoan that is of importance to poultry production
Definition
Coccidia
Term
What are the main differences between bacteria and archaea?
Definition
-composition of cell wall; bacteria use peptidoglycan in their cell wall while archaea don't
-archaeans possibly have no cell wall
Term
What are some of the phenotypic characteristics that can be used to classify bacteria?
Definition
-Morphology and staining
-Oxygen requirements
-Biochemical tests
-Serological systems
-Environmental reservoirs
-Fatty acid profiles
Term
What are the four main steps of Gram staining and what are the reagents used?
Definition
1: Applying a primary stain (crystal violet) to a heat- fixed smear of a bacterial culture
2: The addition of iodide, which binds to crystal violet and traps it in the cell
3: Rapid decolorization with ethanol or acetone
4: Counterstaining with safranin
Term
What are the two main differences between the cell wall of Gram positive and negative bacteria?
Definition
[image]
Term
What are the major sources of microbial contamination of muscle foods (fresh meats)?
Definition
-Environment
-Gastrointestinal contents
-Air in the processing
-Personnel in production and processing operations
Term
What are the two sources of microbial contamination during processing of fresh poultry?
Definition
-Majority from the growing environment
-Gastrointestinal contents

verify with Dr. Thippareddi
Term
What are the two sources of microbial contamination during processing of processed meat and poultry products?
Definition
-Ingredients
-Environmental contamination (equipment, personnel or air)

verify with Dr. Thippareddi
Term
microbial succession
Definition
the change in a bird's microflora as the bird ages
Term
Why are there differences in the microbial types in different regions of the poultry GI tract?
Definition
because of different conditions in different parts of the GI tract
Term
What are the standards for the National Primary Drinking Water Regulations based on?
Definition
-Heterotrophic Plate Count (HPC) -Total Coliforms (<5%; including fecal coliforms and E. coli) -Turbidity
Term
What are the organisms that are used in the standards for the National Primary Drinking Water Regulations?
Definition
-Cryptosporidium -Giardia lamblia -viruses (enteric) -Legionella -Coliforms, including fecal coliforms and E. coli
Term
What are the 8 principles of biosecurity?
Definition
-Keep visitors to a minimum
-Limit visitations to other poultry farms
-Keep all animals out of poultry houses
-Practice sound rodent and pest control programs
-Avoid contact with non-commercial poultry or wild birds
-Inspect flocks daily
-Maximize the environment (dry litter and good ventilation)
-Keep areas around houses and feed bins clean
Term
How do transportation crates contribute to the microbial load of the poultry?
Definition
-cross-contamination occurring in dirty transportation coops/crates -feces from birds in higher crates can get onto birds in lower crates (Salmonella and Campylobacter)
Term
Does washing reduce (significantly) the microbial load of the poultry from transportation?
Definition
yes; gets rid of the organic matter of the feces
Term
Other than washing, what else can be done to reduce the microbial load on poultry from transport?
Definition
-use the right sanitizers to kill the bacteria that are present there
-dry the crates so the organisms can't survive very long
Term
What is the significance of the microbial load in the skin?
Definition
-it can contain Salmonella and Campylobacter -sanitizers won't kill pathogens that get into the skin
Term
intrinsic parameters
Definition
Parameters that are inherent to the food product that affect microbial behavior
Term
examples of intrinsic parameters
Definition
-pH
-moisture content or water activity
-Oxidation-reduction potential (Eh)
-Nutrient content
-Antimicrobial constituents
-Biological structures
Term
extrinsic parameters
Definition
Parameters that affect microbial behavior, but are related to the environment they are stored in/at
Term
examples of extrinsic parameters
Definition
-Temperature of storage
-relative humidity of the environment
-presence and concentration of gases
-presence and activities of other microorganisms
Term
pH
Definition
The negative logarithm of hydrogen ion concentration in a product

this indicates the acidity (0-7) or the alkalinity (7-14) of a product
Term
how to calculate pH
Definition
pH = -log (H+)
Term
pH scale with some products listed
Definition
[image]
Term
why sodas are acidified (citric or phosphoric acid)
Definition
-kill organisms
-tastier
Term
why egg whites become more alkaline as they get older
Definition
because they lose carbon dioxide
Term
why buttermilk is acidic
Definition
because of lactic acid bacteria and other beneficial organisms using lactose and fermenting
Term
why cheese is acidic
Definition
because it's fermented
Term
why the pH of meat goes down shortly after slaughter
Definition
the glycogen in the muscle is converted to lactic acid, reducing the pH of the muscle
Term
the pH at which microbes tend to grow best
Definition
pH 6-7
Term
group of bacteria that grows best at low pH
Definition
lactic-acid-producing bacteria
Term
pH homeostasis
Definition
maintaining a pH of 7.0 – 7.2 in animal muscle
Term
Post-rigor muscle pH
Definition
normally between 5.6–6.4
Term
At pH <5.4, these bacteria decrease
Definition
Staphylococcus, Micrococcus and Lactobacillus
Term
some microbes that survive the post-rigor acidification of meat
Definition
-yeasts -Pseudomonas (survives acidification, but lag phase increases)
Term
what does Pseudomonas do after the pH of the post-rigor meat goes back above 5.4?
Definition
it quickly dominates and produces putrefactive odors (rather than souring as in normal and PSE meats)
Term
PSE
Definition
pale, soft, and exudated
Term
how does meat become pale, soft, and exudated (PSE)?
Definition
the pH decreases, the meat can't hold the moisture, and thus releases the water, giving the meat a lighter color
Term
the demarcation between low acid and high acid foods
Definition
pH 4.5
Term
For safety (______), the pH of fermented meat products should be ______
Definition
-E. coli O157:H7 -<4.5-4.7
Term
high acid
Definition
pH <4.5
Term
low acid
Definition
pH >4.5
Term
why it's best for acidification of meat products to be quick
Definition
because slow fermentation allows Staphylococcus aureus to grow and produce enterotoxin
Term
why it's better to measure water activity (aw) than to measure water content
Definition
reflects better on the potential of the growth for microbes
Term
What is the primary difference between moisture content and the water activity of a food product?
Definition
moisture content is a matter of how much water is in it while water activity is a matter of how much water is available for the microbes to grow
Term
low water activity
Definition
<0.85
Term
ways to measure the water activity
Definition
-relative humidity of the food product
-relative humidity of that product in a closed environment
-dew point measurement
Term
the demarcation between high water activity and low water activity
Definition
0.86
Term
some low water activity foods (<0.85 aw) that were implicated in foodborne illness
Definition
-cereals
-chocolate
-cocoa powder
-dried fruits
-fermented dry sausage
-flour
-herbs
-spices
-hydrolyzed vegetable protein powder
-dried meat
-peanut butter
-tree nuts
-powdered infant formula (PIF)
Term
why dry food products can be linked to foodborne infections
Definition
bacteria, such as Salmonella, surviving, but not growing
Term
Oxidation-Reduction Potential (Eh)
Definition
Ease with which a substrate loses or gains electrons
Term
Oxidation-Reduction Potential measurements are affected by...
Definition
all oxidizing and reducing agents, not just acids or bases (as in pH)
Term
______ organisms require a positive Eh values (oxidized) for growth
Definition
Aerobic
Term
______ organisms require negative Eh values (reduced) conditions for growth
Definition
Anaerobic
Term
What substances in foods contribute to reducing conditions?
Definition
-Substances/proteins that are high in sulfhydryl (–SH) group (amino acid cysteine, ascorbic acid, reducing sugars in fruits and vegetables)
-growth of microbes
Term
Oxidation-Reduction (OR) Potential of a food is determined by...
Definition
-Food product
-Poising capacity of the food
-Oxygen tension in the environment
-Barrier to the environment (e.g. packaging)
Term
the Oxidation-Reduction Potential (Eh) values immediately after slaughter
Definition
positive ~250 mV
Term
the post-rigor Oxidation-Reduction Potential (Eh) values
Definition
reduced to ~30 mV
Term
example of microbes reducing their medium's oxidation-reduction potential
Definition
aerobic organisms can grow in microbiological media, reduce the medium allowing anaerobic organisms to grow
Term
For growth, microorganisms require
Definition
-Water
-Source of energy
-Source of nitrogen
-Vitamins and related growth factors
-Minerals
Term
the organisms with the lowest requirements for growth
Definition
molds, followed by G- bacteria, yeasts, and G+ bacteria
Term
some antimicrobial constituents in foods
Definition
-Lactoperoxidase system in milk
-Essential oils, such as cinnamaldehyde and oregano oil, in spices
-Lactoferrin in milk
-lysozyme and conalbumin in egg white
Term
how lactoferrin is antimicrobial
Definition
it binds iron
Term
why essential oils are added to the no antibiotics ever chickens
Definition
because it prevents the growth of bac bacteria in the gut
Term
how conalbumin is antimicrobial
Definition
it binds nutrients required for growth of microbes
Term
examples of structures in food that may prevent microbial invasion
Definition
-Egg shell
-shell membrane
-banana skin
Term
psychrophiles
Definition
-“Cold-loving”
-Can grow at 0°C
-Optimum growth at 15°C or below.
-Found in very cold environments (North pole, ocean depths).
-Seldom cause disease or food spoilage.
Term
mesophiles
Definition
-Grow best at moderate temperatures (20-45°C); optima 30-40°C
-Include most pathogens and common spoilage organisms.
-Many have adapted to live in the bodies of animals.
Term
thermophiles
Definition
-Optimum growth between 55 to 65°C.
-Grow well above 45°C.
-Adapted to live in sunlit soil, compost piles, and hot springs.
-Some thermophiles form extremely heat resistant endospores.
Term
psychrotrophs
Definition
-Optimum growth at 20 to 30°C; but can grow well at or below 7°C.
-Responsible for most low temperature food spoilage.
Term
What is the difference between psychrophile and a psychrotroph?
Definition
psychrophiles grow best at low temperatures while psychrotrophs are mesophiles that can grow at body temperature and low temperature
Term
some examples of psychrotrophs
Definition
-Alcaligenes -Shewanella -Brochothrix -Corynebacterium -Flavobacterium -Lactobacillus -Micrococcus -Pectobacterium -Pseudomonas -Psychrobacter -Enterococcus -others
Term
the temperature group of bacteria responsible for most low temperature food spoilage
Definition
psychrotrophs
Term
some lactic acid bacteria that are psychrotrophs
Definition
-Brochothrix -Lactobacillus
Term
a psychrotroph that causes putrefication
Definition
Pseudomonas
Term
the temperature group of bacteria that includes most pathogens and common spoilage organisms
Definition
mesophiles
Term
some examples of thermophiles
Definition
-Bacillus -Paenibacillus -Clostridium -Geobacillus -Alicyclobacillus -Thermoanaerobacter
Term
Extreme Thermophiles (Hyperthermophiles)
Definition
Optimum growth at 80°C or higher. Archaebacteria. Most live in volcanic and ocean vents.

we don't really deal with these in food products
Term
what's number 1?
[image]
Definition
psychrophiles
Term
what's number 2?
[image]
Definition
psychrotrophs
Term
what's number 3?
[image]
Definition
mesophiles
Term
what's number 4?
[image]
Definition
thermophiles
Term
what's number 5?
[image]
Definition
extreme thermophiles
Term
some types of modified atmospheres used for packaging meat
Definition
-Aerobic
-Vacuum
-High oxygen
-Carbon monoxide
Term
composition of high oxygen modified atmosphere
Definition
-80% O2 -20% CO2
Term
composition of carbon monoxide modified atmosphere
Definition
-0.5% CO -60% CO2 -39.5% N2
Term
organisms that may be able to grow in a vacuum modified atmosphere
Definition
-anaerobes
-facultative anaerobes
Term
Advantages of modified atmosphere packaging
Definition
-Increase in shelf life (50-400%)
-Reduced economic loss
-Products can be distributed over longer distances and fewer deliveries – reduced distribution costs
-Higher quality product
-Easier separation of slices
Term
the modified atmosphere used for shipping meat and poultry products to east Asia and other far-away places
Definition
vacuum-packaged
Term
Disadvantages of modified atmosphere packaging
Definition
-Added cost
-Temperature control necessary
-Special equipment and training necessary
Term
how the packaging atmosphere affects meat color (memorize)
Definition
[image]
Term
why high oxygen (80% O2 / 20% CO2) modified atmosphere packaging is sometimes used
Definition
to keep the cherry red color for a longer time
Term
disadvantage of using high oxygen (80% O2 / 20% CO2) modified atmosphere packaging
Definition
the extra oxygen leads to fatty acid oxidation and thiobarbituric acid production
Term
Some substances organisms can produce to inhibit or prevent the growth of other bacteria
Definition
-Antibiotics
-Bacteriocins
-Hydrogen peroxide
-Organic acid
Term
Bacteriocins
Definition
A protein produced by bacteria of one strain and active against those of a closely related strain
Term
Hurdle concept
Definition
use of intrinsic or extrinsic parameters to prevent or slow down the growth of microbes of concern
Term
Give examples of modified atmospheres used to improve shelf life of foods
Definition
-aerobic
-vacuum
-high oxygen
-carbon monoxide
Term
What is the principle behind low temperature preservation of foods?
Definition
Reducing product temperature prevents growth of mesophilic organisms in food – extension of shelf life and less foodborne illness -Pathogens like S. aureus will not be able to grow
Term
Why are they important in refrigerated foods?
Definition
because they're responsible for most low temperature food spoilage.
Term
Prior to invention of mechanical refrigeration, food spoilage was mostly by...
Definition
mesophilic organisms
Term
some ways to increase food shelf life other than refrigeration
Definition
-cooking
-drying
Term
Prior to invention of mechanical refrigeration, ______ was used to cool foods
Definition
ice
Term
history of commercial refrigeration
Definition
-Early forms of cooling – 1000 BC, such as Chinese, Jewish, Greek, Roman, Egyptian and Persian writings
-Ice harvesting (1800’s)
-Refrigerated railroad cars used for dairy products and meat introduced in the US – 1840s
-1900s – Major meat packing houses installed refrigeration system in packing houses and the regional branches for better manufacturing and distribution
Term
some Chemical/biochemical signs of food spoilage
Definition
-Odors
-acid
-putrid
Term
some Visual/Sensory signs of food spoilage
Definition
Color, e.g., ground beef
Term
some Microbiological signs of food spoilage
Definition
-Index of spoilage
-~7.0 log CFU
+Types of spoilage organisms can make a difference
Term
What happens to microbes when you freeze the meat they're on?
Definition
-Slower metabolic rate
-Membranes transport solutes more efficiently
-Produce larger cells
-Flagella synthesis is more efficient
-Faster growth rate with oxygenation
-Increased requirement for organic nutrients
Term
some parameters to consider in modified atmosphere packaging
Definition
Permeability of the packaging film – oxygen, moisture and at what temperature
Term
High Oxygen MAP
Definition
70% O2; 20-30% CO2 and 0-20% N2
Term
low oxygen MAP
Definition
10% O2; 20-30% CO2 and the rest is N2
Term
what the carbon monoxide modified atmosphere is used for and why
Definition
Only in beef applications – color implications
Term
primary effects of Food Preservation with Modified Atmospheres
Definition
-Greater inhibition at lower temperatures (additive effect, greater solubility of CO2) -Greater inhibition at lower pH -G- organisms are impacted more than G+ organisms (E.g. Pseudomonas) -Both lag phase duration and growth rate are affected -Greater pressure – greater activity
Term
Spoilage of MAP Packaged Products is Determined by...
Definition
-Product type (raw or cooked)
-Concentration of antimicrobials (nitrites, lactates & diacetates, etc.)
-Other psychrotrophs (LAB)
-Barrier properties of the packaging film
-Product pH
Term
What are the two microbial pathogens of concern in modified atmosphere packaged poultry products?
Definition
-C. botulinum -L. monocytogenes
Term
Microorganisms and enzymes need ______ for their activity
Definition
water
Term
types of drying
Definition
-Traditional atmospheric (Spray, Drum)
-Vacuum
-Freeze (lyophilization, cryophilization)
Term
how to calculate water activity
Definition
aw = P / P0 = ERH / 100 -P = partial vapor pressure of food moisture at temperature T -P0 = Saturation vapor pressure of pure water at T -ERH = Equilibrium relative humidity at T
Term
Triple Point
Definition
Temperature and pressure at which the three phases of the substance coexist in thermodynamic equilibrium
Term
Critical Point
Definition
Temperature and pressure where the density of the liquid and gas phases are the same
Term
Intermediate Moisture Foods
Definition
-Moisture between 15-50%
-Water activity between 0.60 and 0.85
Term
pathogen of concern in Intermediate Moisture Foods
Definition
S. aureus
Term
some ways to adjust moisture content
Definition
-Adjust aw using humectants -Antifungal agents (yeasts and molds) -May be pH adjustment
Term
Radiation typically used in food preservation
Definition
-Gamma
-X-rays
-UV
Term
UV light
Definition
-Powerful bactericidal agent
-Used to sanitize surfaces, clear liquids
[image]
Term
Which of the UV spectrum (wavelengths) is used for antimicrobial activity?
Definition
UV C
Term
Which of the UV spectrum (wavelengths) is used for tanning?
Definition
UV A
Term
2 microbes that can grow in vacuum-packaged products
Definition
-Brochothrix -Lactobacillus
Term
how microbes contribute to food spoilage
Definition
-they grow to a certain level
-produce enzymes that can break down fats, proteins, and carbohydrates
Term
how a food product can become sour as it spoils
Definition
bacteria grow and produce enzymes that can degrade carbohydrates, resulting in acidification
Term
What is index of spoilage in terms of microbiological load of meat and poultry products?
Definition
~7.0 log CFU
-Types of spoilage organisms can make a difference
Term
how food with a microbial load above ~7.0 log CFU can be not spoiled
Definition
it can be fermented such that it's preserved, such as yogurt and fermented meat
Term
some psychrotrophic bacteria that are foodborne pathogens
Definition
[image]
Term
does freezing kill the bacteria?
Definition
not really
they go dormant, but don't grow
Term
some parameters to consider in vacuum packaging
Definition
-Permeability of the packaging film
-oxygen
-moisture
-temperature
-oxygen, moisture, and maybe even permeability are specific at specific temperatures
-Changes in the atmosphere can occur over time or during storage
Term
the most important properties to consider in the packaging material for modified atmosphere packaging
Definition
-oxygen transmission rate
-water vapor transmission rate
Term
what determines the type of packaging you wanna use for a meat product?
Definition
the cost/benefit ratio
Term
some food products in which Clostridium botulinum can be a problem and why
Definition
Cooked, prepared products because of spores that survive the packaging and canning processes and grow under anaerobic conditions
Term
ways to prevent the germination of Clostridium botulinum spores in anaerobic conditions
Definition
-Water activity >0.93
-pH >4.6
-IMPORTANT: if one of these criteria is not met, the spores can germinate, leading to growth and cause of illness
Term
how to slow down the growth of Listeria monocytogenes
Definition
-Storage temperature
-antimicrobial use
Term
shelf life is determined by...
Definition
-the intrinsic and extrinsic parameters of the food
-how the food was processed
Term
how drying food products prevents growth of microbes
Definition
Microorganisms and enzymes need water for their activity
Term
how drum drying is done
Definition
the product is applied to a very hot drum as a paste or film on top and scraped off of the drum when the product is sufficiently dry
Term
example of a food product that's drum-dried
Definition
milk powder
Term
how spray drying is done
Definition
mixture containing food product is sprayed in such small droplets, that the moisture removal is instantaneous, resulting in a dry food product
Term
vacuum drying
Definition
reducing the air pressure to lower the water's boiling point
Term
freeze drying aka...
Definition
-lyophilization
-cryophilization
Term
water activity
Definition
measure of the water available to microbes
Term
What is the relationship of ERH and water activity of a food product?
Definition
proportional
Term
some devices that can be used to measure water activity
Definition
-rotronic relative humidity measurement system
-dew-point measurement system (faster)
Term
difference in adsorption and desorption between high and low moisture content foods
Definition
[image]
notice that the adsorption and desorption curves are different in foods with low water activity
Term
some things drying prevents or slows down in food
Definition
-lipid oxidation
-nonenzymatic browning
-enzyme activity
-fungi
-yeasts
-bacteria
Term
the target water activity if the goal is toprevent microbial growth
Definition
<0.85
Term
table showing product pH, water activity (aw), and control of spores (thermally processed)
Definition
[image]
Term
table showing product pH, water activity (aw), and control of vegetative cells and spores
Definition
[image]
Term
temperature of drying vs. pH of dried product
Definition
inverse
(higher temperature, lower pH)
Term
temperature of drying vs. water activity (aw) of dried product
Definition
proportional
(higher temperature, higher water activity)
Term
Rate of freezing or thawing depends on
Definition
-Temperature differential between product and environment
-Mode of heat transfer (conduction, convection, radiation);
-Type, size, and shape of the package;
-Size, shape, and thermal properties of the product
Term
triple point
Definition
temperature and pressure where the 3 states of the substance (solid, liquid, and gas) coexist in thermodynamic equilibrium
[image]
Term
critical point
Definition
the temperature and pressure where the densities of the liquid and gas are the same
[image]
Term
What principle does the freeze drying use?
Definition
sublimation, in which the ice goes from solid straight to gas
Term
What is the foodborne pathogen that can grow at the lowest water activity and what is the water activity value?
Definition
Staphylococcus aureus 0.86
Term
Intermediate Moisture Foods
Definition
-Moisture between 15-50%
-Water activity between 0.60 and 0.85
Term
the pathogen of main concern in intermediate moisture foods
Definition
Staphylococcus aureus
Term
humectants
Definition
substances that can bind the moisture
Term
some ways to make an intermediate moisture food safer
Definition
-Adjust aw using humectants
-Antifungal agents (yeasts and molds)
-May be pH adjustment
Term
What is an IMF food product?
Definition
intermediate moisture food product
Term
the electromagnetic spectrum, ionizing and non-ionizing radiation
Definition
[image]
Term
sanitizing use of UV light
Definition
-Powerful bactericidal agent
-Used to sanitize surfaces, clear liquids
Term
when UV light doesn't kill bacteria
Definition
when something blocks the light, whether it be a cloth, mask, or whatever it may be
Term
what UV light does to cells
Definition
-Ionizing
-Absorbed by proteins and nucleic acids; mutations
-Photochemical changes, may lead to cell death
Term
disadvantage of using UV light to sanitize food
Definition
POOR penetration power – not ideal for food products
Term
β rays
Definition
-Streams of electrons (radioactive material or cathode rays)
-Ionizing
-MEDIUM penetration power – not ideal for food products
Term
disadvantage of using β rays
Definition
MEDIUM penetration power – not ideal for food products
Term
Gamma rays
Definition
-Emitted from excited nucleus of radioactive materials (60Co, 137Cs) -Ionizing -Inexpensive form for food preservation -EXCELLENT penetration power
Term
why the source of gamma rays (radioactive materials such as 60Co and 137Cs) can be used for a long time
Definition
because its half-life is hundreds of years
Term
the symbol required to be on any food product that is irradiated
Definition
the Radura symbol
[image]
Term
[image]
Definition
the Radura symbol
Term
food products that do not have to be irradiated
Definition
spices
Term
spices that are not irradiated
Definition
organic spices
Term
Of the ionizing radiation, which ones have the best penetration power?
Definition
gamma rays
Term
source of gamma rays
Definition
radioactive materials such as 60Co and 137Cs
Term
some foods that are approved for irradiation treatment
Definition
-beef
-pork
-crustaceans
-fruit
-veggies
-lettuce
-spinach
-poultry
-seeds for sprouting, such as alfalfa sprouts
-shell eggs
-shellfish
-spices
-seasonings
Term
What determines the efficacy/dose of irradiation required?
Definition
-Types of organisms (G+ more resistant)
-Number of organisms (initial microbial load)
-Composition of the food
-Presence or absence of oxygen
-Physical state of food (frozen, thawed, temperature, etc.)
Term
the microbes most resistant to irradiation
Definition
viruses, followed by G+ bacteria
Term
how the physical state of the food product affects the amount of irradiation needed
Definition
frozen food requires more irradiation
Term
Dose ranges of irradiation for various applications (be familiar with this)
Definition
[image]
Term
Radappertization
Definition
-Radiation sterilization (commercial sterility)
-30-40 kGy
Term
Radicidation
Definition
-Pasteurization, kinda similar to canning
-2.5-10 kGy
Term
Radurization
Definition
-Pasteurization (enhance shelf life); not truly pateurization
-0.75-2.5 kGy
Term
kGy
Definition
KiloGray
Term
disadvantage of freeze drying
Definition
it's commercially prohibitive
Term
Radiation is a great method to...
Definition
-Assure food safety;
-Extend shelf life of products
Term
Short-chain fatty acids (SCFA)
Definition
fatty acids with aliphatic tails of fewer than six carbons
Term
Medium-chain fatty acids (MCFA)
Definition
fatty acids with aliphatic tails of 6–12 carbons, which can form medium-chain triglycerides
Term
Long-chain fatty acids (LCFA)
Definition
fatty acids with aliphatic tails 13 to 21 carbons
Term
one bad thing irradiation does to foods
Definition
enhances fatty-acid oxidation
Term
commercial sterility
Definition
a food product being shelf-stable such that the spores of the disease-causing microbes don't grow and cause spoilage
Term
target organisms for irradiation
Definition
-vegetative bacteria
-yeasts
-molds
Term
difference between pasteurized and ultrapasteurized
Definition
temperature and time difference
Term
aseptic packaging
Definition
packaging in an environment that is free of bacteria, often when the food is treated with UV light and hydrogen peroxide
Term
Use by/freeze by dates are usually based on...
Definition
the shelf life of the product
Term
The ground beef the consumer is most likely to buy is...
Definition
the one with the best color
Term
the second criterion of food spoilage
Definition
smell
Term
the smell of a food product is an indicator of...
Definition
chemical changes
Term
Potato chips in the ______ become stale in terms of texture
Definition
heat
Term
Potato chips in the heat become stale in terms of...
Definition
texture
Term
______ become oxidized
Definition
Fats
Term
Formation of ______ in fats is the molecule's attempt to oxidize
Definition
a double bond
Term
Formation of a double bond in ______ is the molecule's attempt to oxidize
Definition
fats
Term
Formation of a double bond in fats is the molecule's attempt to...
Definition
oxidize
Term
this structure in a fatty acid can actually bind to oxygen
Definition
double bond
Term
The number of double bonds in a fatty acid changes depending on...
Definition
the fatty acid
Term
Package that smells putrid is past its...
Definition
shelf life
Term
Shelf life is based on...
Definition
the time it takes for the product to reach the index of spoilage
Term
For ______, the color doesn’t matter as much as it does with ______ and such
Definition
poultry meat
beef
Term
Oxidation slows down significantly when...
Definition
there’s no oxygen available
Term
People are getting past the use by freeze by dates by way of...
Definition
the index of spoilage
Term
Shelf life based on food safety is basically a matter of...
Definition
when it reaches index of spoilage
Term
______ enhances oxidation because of exciting electrons
Definition
Irradiation
Term
Irradiation enhances ______ because of exciting electrons
Definition
oxidation
Term
Irradiation enhances oxidation because of...
Definition
exciting electrons
Term
When measuring ______, the agar has to be sterilized by autoclaving to kill the vegetative microbes
Definition
commercial sterility
Term
When measuring commercial sterility, the ______ has to be sterilized by autoclaving to kill the vegetative microbes
Definition
agar
Term
When measuring commercial sterility, the agar has to be sterilized by...
Definition
autoclaving to kill the vegetative microbes
Term
a type of bacteria that forms endospores
Definition
Clostridium botulinum
Term
what endospores can do in canned food products
Definition
-germinate
-grow
-produce gas
-make the cans explode
Term
why commercial sterility must use different processes for different target places
Definition
because of different conditions in different places
Term
a food product that can be a risk for listeriosis
Definition
processed meat
Term
Listeriosis is causes by...
Definition
Listeria monocytogenes
Term
some things in meat processing that can carry Listeria monocytogenes
Definition
-Equipment
-blades
-processing and packaging equipment
-Gunk containing this can get into the walls
Term
some things in the poultry house that can carry pathogens
Definition
-Litter
-air
-water
-feed
-Soil under the litter
-Insects, especially beetles
Term
______ water should be free of E. coli
Definition
Potable
Term
Potable water should be free of...
Definition
E. coli
Term
Bird feces can have this many bacteria per gram
Definition
1010
Term
Insects, especially beetles, can carry pathogens, especially ______, since beetles eat feces
Definition
Salmonella
Term
Insects, especially beetles, can carry pathogens, especially Salmonella, since beetles...
Definition
eat feces
Term
hard or easy to control beetles?
Definition
very hard
Term
one way chickens can ingest Salmonella
Definition
by eating beetles
Term
The ______ of the bird can carry Salmonella and Campylobacter and other pathogens into the processing plant
Definition
feathers and skin
Term
The feathers and the skin of the bird can carry ______ and other pathogens into the processing plant
Definition
Salmonella, Campylobacter
Term
The role of myoglobin
Definition
to have oxygen available for the muscle
Term
why ground meat is bright red
Definition
because the myoglobin in the muscle binds with atmospheric oxygen
Term
As the piece of meat sits on the shelf, the oxymyoglobin in it loses oxygen and becomes...
Definition
metmyoglobin
Term
Exposing deoxymyoglobin to air causes it to become...
Definition
oxymyoglobin
Term
why CO is poisonous
Definition
because it outcompetes oxygen on the hemoglobin and myoglobin
Term
______ is reddish-pink, lasting for maybe about 28 days
Definition
Carboxymyoglobin
Term
Carboxymyoglobin is [this color], lasting for maybe about 28 days
Definition
reddish-pink
Term
Carboxymyoglobin is reddish-pink, lasting for maybe about...
Definition
28 days
Term
how sugar lowers water activity (aw)
Definition
by binding to water
Term
some things that can decrease water activity
Definition
-sugar
-salt
Term
The sour taste in pepperoni comes from...
Definition
the lactic acid that results from fermentation
Term
some pathogens we’re seeing some antibiotic resistance in
Definition
-Campylobacter -Salmonella
Term
The necessary water activity for Staphylococcus aureus
Definition
0.86
Term
The lowest tolerable pH for C. botulinum to germinate and grow
Definition
4.6
Term
why the demarcation between high and low acid according to the FDA is 4.5
Definition
because the lowest tolerable pH for C. botulinum to germinate and grow is 4.6
Term
the demarcation between high and low acid according to the FDA
Definition
4.5
Term
The pH of veggies is usually...
Definition
pretty high
Term
______ are used for pickling
Definition
Vinegar and salt
Term
Vinegar and salt are used for...
Definition
pickling
Term
Pickles are ______ acid
Definition
high
Term
Pickling
Definition
adding vinegar and/or other acid to reduce the pH
Term
Canning is for this pH
Definition
over 4.5
Term
Canning
Definition
sealing it and heating it for 450 deg F for 15 minutes or longer to kill the spores
Term
Stuff in these cans is riskier to eat
Definition
bulged or deformed cans
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